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OfflineMxkingjp
Stranger
Registered: 06/24/20
Posts: 12
Last seen: 3 days, 8 hours
Liquid Culture Contaminated?
    #26790728 - 06/28/20 07:13 PM (3 years, 6 months ago)

Hey guys!

This is my first post and I'm about to start my first grow so I purchased some supplies from the site sponsored vendors. Some of the things I bought were a spore syringe and a sterilized liquid culture jar that's supposed to be pre sterilized that I wanted to inject with the left over liquid from using the syringe.

The question I have is if the jar looks contaminated or if they normally look cloudy like this?
http://imgur.com/a/bT21auI


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InvisibleShenmue
Dark Lord of the Sith 
Registered: 12/21/18
Posts: 2,514
Re: Liquid Culture Contaminated? [Re: Mxkingjp]
    #26790731 - 06/28/20 07:15 PM (3 years, 6 months ago)

Why didn't you just use agar?


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OfflineMxkingjp
Stranger
Registered: 06/24/20
Posts: 12
Last seen: 3 days, 8 hours
Re: Liquid Culture Contaminated? [Re: Shenmue]
    #26790749 - 06/28/20 07:25 PM (3 years, 6 months ago)

I also bought some agar mix to try that too I just wanted to try the LC also.


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Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Liquid Culture Contaminated? [Re: Mxkingjp]
    #26791026 - 06/28/20 10:09 PM (3 years, 6 months ago)

cloudy = contam

spores to lc = contam

its better to only inoculate lc with a clean growth agar wedge.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

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OfflineMxkingjp
Stranger
Registered: 06/24/20
Posts: 12
Last seen: 3 days, 8 hours
Re: Liquid Culture Contaminated? [Re: sandman420]
    #26791590 - 06/29/20 08:12 AM (3 years, 6 months ago)

That's what I feared.. But thank you very much! I guess I'll sterilize the jar and make an agar culture and then try that.


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