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OfflinexXxM1NG0xXx
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Registered: 07/27/12
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Questions on monokaryotic mycelium for crossing
    #26789421 - 06/28/20 10:13 AM (3 years, 6 months ago)

i. Is it possible to achieve monokaryotic mycelium through a tissue sample from a agar plate via sectoring?

ii. Is monokaryoric only possible through spore isolation?

iii. Is tomentose mycelium from a tissue sample monokaryotic?


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InvisiblebodhisattaMDiscordReddit
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Re: Questions on monokaryotic mycelium for crossing [Re: xXxM1NG0xXx]
    #26789503 - 06/28/20 10:44 AM (3 years, 6 months ago)

1. No
2. Yes. Single spore isolation. Or serial dilution to achieve the same.
3. No


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OfflinexXxM1NG0xXx
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Re: Questions on monokaryotic mycelium for crossing [Re: bodhisatta]
    #26789531 - 06/28/20 10:57 AM (3 years, 6 months ago)

Perfect, preciate'it, cleared up a bunch of things for me


Edited by xXxM1NG0xXx (06/28/20 10:58 AM)


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Offlinejcm4620
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Re: Questions on monokaryotic mycelium for crossing [Re: xXxM1NG0xXx]
    #26789539 - 06/28/20 11:00 AM (3 years, 6 months ago)

what happened to the avatar bod 😃


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InvisiblebodhisattaMDiscordReddit
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Re: Questions on monokaryotic mycelium for crossing [Re: jcm4620]
    #26789568 - 06/28/20 11:10 AM (3 years, 6 months ago)

Flagged as adult. Gotta go into settings and allow adult images to see it


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Offlinejcm4620
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Re: Questions on monokaryotic mycelium for crossing [Re: bodhisatta]
    #26789645 - 06/28/20 11:44 AM (3 years, 6 months ago)

ahh i got it now lol


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InvisiblePastywhyteMDiscord
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Re: Questions on monokaryotic mycelium for crossing [Re: jcm4620]
    #26789672 - 06/28/20 12:06 PM (3 years, 6 months ago)

There is a method of dedikarotization where dikarotic culture is blended in a saline solution to separate the genetics. They can then be diluted, plated, and selected. I know of a guy who was doing this with Pleurotus and Trichoderma in an effort to isolate specific traits in monokaryons to be mated to produce custom strains. He was then using those strains in other experiments concerning trich contamination in Pleurotus colonies.

I know he was considering trying them with cubes and he was working with a cube grower to try and create an entry level method for people to follow. I haven’t talked to either of them recently, I don’t think they have accounts here.


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Offlinejcm4620
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Re: Questions on monokaryotic mycelium for crossing [Re: Pastywhyte]
    #26790073 - 06/28/20 02:56 PM (3 years, 6 months ago)

well pasty the good news is that there is plenty of us other expert trich cultivators here😂😂😂


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