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OfflineVibe_Enthusiast
Mushroom Technician
I'm a teapot


Registered: 10/16/18
Posts: 2,420
Loc: GPS signal lost.. Flag
Last seen: 18 days, 20 hours
I seek your advice, grasshoppa
    #26785131 - 06/26/20 04:02 PM (3 years, 6 months ago)

What's up my beautiful people! Sooo, kinda have been wanting to do another grow! I came across this dish I had in my cabinet of my room! From 5 months ago I let a culture grow out on here! Forgot all about it lol.

Not that I'm trying to cut edges or anything - I still have agar and syringes in the fridge. But I was wondering.. if I could drop a wedge from this to one jar and do a g2g to some others!?

I'm not sure how long is "acceptable" for things of such nature. It has been sitting in my room. Hasn't been touched!



:cheers:


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:greyalien:




Edited by Vibe_Enthusiast (06/26/20 04:03 PM)


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: I seek your advice, grasshoppa [Re: Vibe_Enthusiast]
    #26785225 - 06/26/20 04:52 PM (3 years, 6 months ago)

Quote:

Vibe_Enthusiast said:
What's up my beautiful people! Sooo, kinda have been wanting to do another grow! I came across this dish I had in my cabinet of my room! From 5 months ago I let a culture grow out on here! Forgot all about it lol.

Not that I'm trying to cut edges or anything - I still have agar and syringes in the fridge. But I was wondering.. if I could drop a wedge from this to one jar and do a g2g to some others!?

I'm not sure how long is "acceptable" for things of such nature. It has been sitting in my room. Hasn't been touched!



:cheers:





I think its hard for anyone to answer this because we know nothing about this plate. I dont think you know anything about this plate either. You can always put a plate to a jar and see what happens. But doing g2g with a plate that is an unknown (to you and to us) and then multiplying it to a bunch of other jars seems like a fun experiment... if you don't mind losing a bunch of jars that is (or not).

As far as I understand g2g is an excellent tek when you have lowered the risk for the original jar as much as possible. The myc doesnt look dead. I wonder why it hasn't pinned after that long though? Or do invitro pins need unwrapped plates for FAE? Would love to know if someone knows about this.


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OfflineTrichodingus
Stranger


Registered: 05/31/20
Posts: 85
Loc: Communing with the divine
Last seen: 3 years, 6 months
Re: I seek your advice, grasshoppa [Re: Vibe_Enthusiast]
    #26785235 - 06/26/20 04:57 PM (3 years, 6 months ago)

If you've got plates already, I'd do a transfer for peace of mind...

Do you really want to find out it's contaminated after it's already on grain?


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: I seek your advice, grasshoppa [Re: Trichodingus]
    #26785257 - 06/26/20 05:07 PM (3 years, 6 months ago)

Quote:

Trichodingus said:
If you've got plates already, I'd do a transfer for peace of mind...

Do you really want to find out it's contaminated after it's already on grain?




Finding out its contaminated after its in one grain jar is one thing, but in several jars... not fun. So, yeah definitely transfer to new plates and see.


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OfflineVibe_Enthusiast
Mushroom Technician
I'm a teapot


Registered: 10/16/18
Posts: 2,420
Loc: GPS signal lost.. Flag
Last seen: 18 days, 20 hours
Re: I seek your advice, grasshoppa [Re: redhandmat]
    #26785413 - 06/26/20 06:21 PM (3 years, 6 months ago)

Yeah you guys are right lol. I'm just gonna be the little safety hazard guy I was when I first started the hobby and be sterile and efficient. I appreciate the replies and talking sense into me:lol:


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:greyalien:




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OfflineGastronomicus
3-0-G
Male User Gallery


Registered: 03/31/05
Posts: 9,727
Last seen: 33 minutes, 24 seconds
Re: I seek your advice, grasshoppa [Re: Vibe_Enthusiast]
    #26785449 - 06/26/20 06:39 PM (3 years, 6 months ago)

I see hyphal knots. Why not wait for it to pin and then make a new dish with the pin?


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OfflineVibe_Enthusiast
Mushroom Technician
I'm a teapot


Registered: 10/16/18
Posts: 2,420
Loc: GPS signal lost.. Flag
Last seen: 18 days, 20 hours
Re: I seek your advice, grasshoppa [Re: Gastronomicus]
    #26785548 - 06/26/20 07:22 PM (3 years, 6 months ago)

Haha man it has been 5 months! Who knows when or if it would ever pin:lol:


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:greyalien:




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