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OfflinePokazu
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I shaked my bags and its not colonizing?!
    #26783481 - 06/26/20 12:07 AM (3 years, 7 months ago)

I shaked my bags at 40% 3 of them and only one just start growing fast, the other 2 stopped with the colonization process what should I do? Should I inject again spores? GT


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Invisiblenix21
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Re: I shaked my bags and its not colonizing?! [Re: Pokazu]
    #26783539 - 06/26/20 01:50 AM (3 years, 7 months ago)

Post pics


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OfflinePokazu
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Re: I shaked my bags and its not colonizing?! [Re: nix21]
    #26785223 - 06/26/20 04:50 PM (3 years, 7 months ago)

sure, as you can see in order the first picture is colonizing fast after the shake, but in the other hand the other 2 pictures stop colonizing they are only left with some white spots.



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Offlineredhandmat
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Re: I shaked my bags and its not colonizing?! [Re: Pokazu]
    #26785265 - 06/26/20 05:09 PM (3 years, 7 months ago)

How long has it even been since you shook the grains until you took the pictures?


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OfflinePokazu
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Re: I shaked my bags and its not colonizing?! [Re: redhandmat] * 1
    #26785312 - 06/26/20 05:30 PM (3 years, 7 months ago)

it has been only 5 days since I shake them, should I inject again spores?


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OfflinePhrontist
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Re: I shaked my bags and its not colonizing?! [Re: Pokazu]
    #26785336 - 06/26/20 05:44 PM (3 years, 7 months ago)

40% is much earlier than I’d break up a bag, but even then, if they hadn’t started to bounce back after 5 days, I would assume there’s something else in the bag preventing it (bacteria).


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InvisibleFriedEggS
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Registered: 09/22/14
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Re: I shaked my bags and its not colonizing?! [Re: Pokazu]
    #26785483 - 06/26/20 06:56 PM (3 years, 7 months ago)

Quote:

Pokazu said:
I shaked my bags at 40% 3 of them and only one just start growing fast, the other 2 stopped with the colonization process what should I do? Should I inject again spores? GT



no don't inject spores again. your bags failed because you injected spores into grain. learn agar and you'll have cleaner growth :thumbup:


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Offlineredhandmat
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Re: I shaked my bags and its not colonizing?! [Re: FriedEgg]
    #26786281 - 06/27/20 03:17 AM (3 years, 7 months ago)

:whathesaid:


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Offlinemushpunx
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Re: I shaked my bags and its not colonizing?! [Re: redhandmat]
    #26786314 - 06/27/20 04:04 AM (3 years, 7 months ago)

Grains that fail to recover or stall after a shake are most likely infected with bacteria or mold (or both).

Spore prints are taken from fruits that were grown in open air, they will never be %100 sterile, and by extension neither will the spore syringes made from them. By using fruits grown indoors and following sterile procedure we can make spore syringes that are clean enough for BRF cakes, which are  pretty forgiving, and in my opinion this is the only real use for spore syringes over prints.

It's possible to grow on grains using an injection from a spore syringe but it is high risk for contamination, sooner or later you will lose grows this way.

Like the others said, if you want to use grain spawn successfully, learn agar first.


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OfflinePokazu
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Re: I shaked my bags and its not colonizing?! [Re: mushpunx]
    #26787722 - 06/27/20 04:07 PM (3 years, 7 months ago)

😔😔 I will never shake a bag again, I dont know I do not use agar because I think its going to take me a lot of space.


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OfflineA.k.aM
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Re: I shaked my bags and its not colonizing?! [Re: Pokazu]
    #26787745 - 06/27/20 04:26 PM (3 years, 7 months ago)

Shaking the bag didn’t do anything but let you know it was contaminated sooner. That first bag is pretty bad also, lotta bacteria but it could still work.

If you hadn’t shaken them they would’ve just gone bad in a week or two.


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LAGM2020


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OfflinePokazu
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Re: I shaked my bags and its not colonizing?! [Re: A.k.a]
    #26787837 - 06/27/20 05:13 PM (3 years, 7 months ago)

well I think I need to level up my game with the spawn, Next time I Will try jars with agar. I just inoculate 2 bags to se what happens but well. The first bag has a lot of mycellium piss


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OfflineReverance
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Registered: 05/07/20
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Re: I shaked my bags and its not colonizing?! [Re: Pokazu]
    #26787877 - 06/27/20 05:32 PM (3 years, 7 months ago)

Quote:

Pokazu said:
I shaked my bags at 40% 3 of them and only one just start growing fast, the other 2 stopped with the colonization process what should I do? Should I inject again spores? GT




bro what temp are you keeping the bags at?

also this thread is kinda wacky to me. @FriedEgg , isnt it pretty commonplace to start spores on grain? I mean yes you have a higher chance of contam than if used agar but it works. I usually shake my bags at 20% and dont have an issue. My major prob I had was I let my temps get too high and growth just stopped in a few of my bags. I had one that colonized a whole bag inspite of very high temps, but like i said, all the others stalled.


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OfflinePokazu
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Re: I shaked my bags and its not colonizing?! [Re: Reverance]
    #26787925 - 06/27/20 05:55 PM (3 years, 7 months ago)

they are around 26-29c with 90% of humidity, I put them in the garden in my house it has a roof of protection.


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OfflineReverance
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Re: I shaked my bags and its not colonizing?! [Re: Pokazu]
    #26787993 - 06/27/20 06:17 PM (3 years, 7 months ago)

i
Quote:

Pokazu said:
they are around 26-29c with 90% of humidity, I put them in the garden in my house it has a roof of protection.




idk, if the temps exceed 29 you might have issues.were still talkin bags right? shoot for 25, you might have better luck


imo.


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InvisibleFriedEggS
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Registered: 09/22/14
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Re: I shaked my bags and its not colonizing?! [Re: Reverance]
    #26788098 - 06/27/20 06:58 PM (3 years, 7 months ago)

Quote:

Pokazu said:
also this thread is kinda wacky to me. @FriedEgg , isnt it pretty commonplace to start spores on grain? I mean yes you have a higher chance of contam than if used agar but it works. I usually shake my bags at 20% and dont have an issue. My major prob I had was I let my temps get too high and growth just stopped in a few of my bags. I had one that colonized a whole bag inspite of very high temps, but like i said, all the others stalled.




yea, a lot of people have success starting spores on grain until they don't. it works sometimes but it's just not reliable. it's so unreliable that most (maybe all?) experienced growers reach a point where they stop trying completely and just decide to use agar. most grain that was inoculated with spores is bacterial. sometimes you'll still get fruits with spores+grain but you'll often run into random problems like a few of your bags stalling at 70%. because you had a few successes with it in the past, you'll think it's a problem with your temps being too high or your sterile technique is off but in reality you probably got an unlucky roll of the dice from your spore syringe. :shrug:

the only time i start spores on grain is when i can't get the spores to germinate on agar. but after they germinate on grain i'll transfer a few kernels of grain to an agar plate to clean up the culture. cleaning up a culture on agar will increase your yields many times over and success will be much more consistent. since i started using clean agar cultures i can't remember the last time i had to throw away a grain jar.


and as long as your spawn is clean and you didn't overhydrate your grain, your bags should colonize fine at 30C. put them in a cooler room if you can but if you don't have an option it's ok. my room doesn't get below 29C in the summer and my spawn colonizes fine.

edit: sorry, you're partially right about the high temps. higher temps will be a problem if your grain has some bacteria in it, for example from a dirty spore syringe. but it's possible to grow in high temps, you just need to use clean spawn and be careful with your grain prep.


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Edited by FriedEgg (06/27/20 07:08 PM)


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OfflinePokazu
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Re: I shaked my bags and its not colonizing?! [Re: FriedEgg]
    #26788132 - 06/27/20 07:23 PM (3 years, 7 months ago)

thankss


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Offlinemushpunx
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Re: I shaked my bags and its not colonizing?! [Re: Pokazu]
    #26788678 - 06/28/20 01:43 AM (3 years, 7 months ago)

Quote:

Pokazu said:
well I think I need to level up my game with the spawn, Next time I Will try jars with agar. I just inoculate 2 bags to se what happens but well. The first bag has a lot of mycellium piss





No such thing as "myc piss". That is metabolites which are excreted in the presence of contamination like bacteria, to try to fight it


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Offlinemushpunx
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Re: I shaked my bags and its not colonizing?! [Re: Reverance]
    #26788694 - 06/28/20 01:56 AM (3 years, 7 months ago)

Quote:

Reverance said:
Quote:

Pokazu said:
I shaked my bags at 40% 3 of them and only one just start growing fast, the other 2 stopped with the colonization process what should I do? Should I inject again spores? GT




bro what temp are you keeping the bags at?

also this thread is kinda wacky to me. @FriedEgg , isnt it pretty commonplace to start spores on grain? I mean yes you have a higher chance of contam than if used agar but it works. I usually shake my bags at 20% and dont have an issue. My major prob I had was I let my temps get too high and growth just stopped in a few of my bags. I had one that colonized a whole bag inspite of very high temps, but like i said, all the others stalled.





It used to be common practice but it was always bad practice. As better information is made available to newcomers  less and less people cultivate that way. 

I don't think you realize just how commonplace bacteria (and often mold) is on spore prints and subsequently spore syringes until you start working with agar and have to transfer clean myc away from it!


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OfflineMycobro420
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Re: I shaked my bags and its not colonizing?! [Re: mushpunx]
    #26789052 - 06/28/20 07:17 AM (3 years, 7 months ago)

Spore syringes can never be 100% clean is true,but for the fact that they are unreliable I beg to differ.i work with only agar now to improve yeilds an make sure my spawn is clean.i have done probly 50+ syringes from sporeworks straight to qt jars of rye. an outta the 100s of jars inoculated that way.I have had mabey 4 jars contaminate an only a few monotubs as well. All got 3 good flushes.soni get where you are coming from with saying they are never 100% clean but I'd say they are still pretty reliable with grain spawn and bulk in qts.iv never inoculated a bag with spores tho.


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