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InvisiblecoAsTal
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Registered: 04/04/06
Posts: 2,970
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26846373 - 07/26/20 03:51 PM (3 years, 6 months ago)

Have a bad feeling about your prospects based on what you've said above bud-- none of them sound clean, and I guess you'll know pretty quick if they contammed out.
:goodluck:


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

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OfflineC12ShroomMan
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26846375 - 07/26/20 03:52 PM (3 years, 6 months ago)

Looking good, how much were those totes?
look ideal height but the shorter ones @ 1$ piece is hard to beat


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Invisiblekrock
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Registered: 12/18/19
Posts: 154
Re: 4th attempt at cleaning up my yeasty spore solution [Re: C12ShroomMan]
    #26847128 - 07/27/20 04:25 AM (3 years, 5 months ago)

yeah I am also concerned about the cultures, I really hope this works out!


The totes aren't as cheap as you're probably used to. I got all three of them for a total of 12 usd (converted from my own currency).
I would love to buy totes at 1$ a piece!

The cheapest totes I can get around here are 5 liter ones that only have transparent lid, rest of the tote is opaque. Cost 2.75$.


Edited by krock (07/27/20 04:57 AM)


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Offlineredhandmat
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Registered: 05/09/19
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26847562 - 07/27/20 10:13 AM (3 years, 5 months ago)

I like the SFF tek too! Fingers crossed mate.

The jars look a liiitle bit weird to me, but if you get at least one fruit then clone it and restart from there.


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Offlineredhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26847568 - 07/27/20 10:15 AM (3 years, 5 months ago)

Quote:

krock said:
yeah I am also concerned about the cultures, I really hope this works out!


The totes aren't as cheap as you're probably used to. I got all three of them for a total of 12 usd (converted from my own currency).
I would love to buy totes at 1$ a piece!

The cheapest totes I can get around here are 5 liter ones that only have transparent lid, rest of the tote is opaque. Cost 2.75$.




Any IKEA near you? IMO the best.


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Invisiblekrock
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Registered: 12/18/19
Posts: 154
Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat]
    #26848302 - 07/27/20 04:15 PM (3 years, 5 months ago)

Wouldn't taking a print from the fruit to restart the gene pool be a better option than cloning the fruit?

Ikea is a bit of a pain to get to because I don't have a car. I go there maybe once a year by bus, lol. Do you know any ikea-totes that are good and cheap?


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InvisiblecoAsTal
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26848324 - 07/27/20 04:28 PM (3 years, 5 months ago)

1) you should ALWAYS get prints of a successful grow, just to make sure you always have resources.
But,
2) you clone good fruits because they've already run the gauntlet of natural selection and won-- they're proven winners. You get that on agar to easily replicate strong, proven mycelium.

Spore prints give you millions of combinations that may or may not be awesome. Nothing wrong with them at all! But clones give you a guarantee that the mycelium has what it takes to go the distance without having to sift through thousands of colonies in a MS culture.


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

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Edited by coAsTal (07/27/20 04:28 PM)


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Invisiblekrock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: coAsTal]
    #26848420 - 07/27/20 05:28 PM (3 years, 5 months ago)

I agree it makes sense to do both a spore print and clone it! I will do both if I actually get some fruits :laugh:

When I wrote about restarting would be better, I was thinking more about what makes sense in terms of what is best for the future. The collection of strains that I have right now in the culture, even if they are able to fruit, are probably kind of shitty because of their origin. These strains just happened to be lucky enough to survive among the contamination. I also don't know how many strains are in there, it could be that there are very few strains and thus very little diversity. My reasoning is that restarting the gene pool with a fresh spore print will give me a better chance of finding a really good culture for future use.


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Offlineredhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26849231 - 07/28/20 03:53 AM (3 years, 5 months ago)

Quote:

krock said:
I agree it makes sense to do both a spore print and clone it! I will do both if I actually get some fruits :laugh:

When I wrote about restarting would be better, I was thinking more about what makes sense in terms of what is best for the future. The collection of strains that I have right now in the culture, even if they are able to fruit, are probably kind of shitty because of their origin. These strains just happened to be lucky enough to survive among the contamination. I also don't know how many strains are in there, it could be that there are very few strains and thus very little diversity. My reasoning is that restarting the gene pool with a fresh spore print will give me a better chance of finding a really good culture for future use.




Exactly bruv, you can do both. Or just the print. You are right on both counts. As far as cloning goes, most growers here would say go for the largest fruit in a cluster.


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Invisiblekrock
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Posts: 154
Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat]
    #26858360 - 08/02/20 08:13 AM (3 years, 5 months ago)

It's time for a small update!


Day 7 shoeboxes



B2T3X2; I am surprised this tote is colonizing the fastest. It was from the jar that I shook and then it didn't grow much. But look at it now colonizing the coir.

B2T3Y2; From the jar that was 100% colonized and had grain cake consistency. It is a bit behind B2T3X2 for some reason.

A4T4; Don't know what to say... It has grey mycelium so it is really hard to see if it is colonizing the coir or not...


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Offlineredhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26858419 - 08/02/20 08:49 AM (3 years, 5 months ago)

Im still not sure about these. The fact that one of the jar didn't recover. And one looked like it never colonized to start with right? But only time will tell.

Hang in there.


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InvisiblePastywhyteMDiscord
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat] * 1
    #26858628 - 08/02/20 11:07 AM (3 years, 5 months ago)

Those all look doomed to me.


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Invisiblekrock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: Pastywhyte]
    #26859989 - 08/03/20 02:52 AM (3 years, 5 months ago)

I agree it doesn't look good. I'll let it ride out, maybe I am lucky and get something.


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Invisiblekrock
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Posts: 154
Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26872763 - 08/10/20 06:18 AM (3 years, 5 months ago)

Update time! Day 15 totes

The top two totes seem to have some kind of black metabolites?







All the totes have begun breaching the casing. When opened all of the totes smelled like coir.

B2T3X2: The casing got a bit wet because there was condensation on the lid that dropped down when I opened up to take the picture.

B2T3Y2: Surface growth actually looks a bit promising.

A4T4: Seems to have come along the most. Surface growth looks whiter than side growth. But growth is kinda loose, mold like?


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InvisiblePastywhyteMDiscord
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock] * 1
    #26872881 - 08/10/20 08:31 AM (3 years, 5 months ago)

All those totes are full of mold. Get them out of the house ASAP. I highly doubt there is any living mushroom mycelium in there at all.


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InvisiblecoAsTal
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: Pastywhyte]
    #26872890 - 08/10/20 08:39 AM (3 years, 5 months ago)

Yeah dude--
:hugitout:


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

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Invisiblekrock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: coAsTal]
    #26873504 - 08/10/20 02:43 PM (3 years, 5 months ago)

FUCK!!

Well at least I tried my best. I feel like I've learned a lot from this experience, I've become better at pouring plates, doing small transfers and also I was able to try out new techniques like proper streaking and hot pour.

With this post I will officially end my efforts in cleaning up that liquid and I will discard the last 1 ml. This has been a wild ride that started out with my first shroomery post 7 months, 18 days ago.
However I am not going to give up on growing cubes. I will acquire a legit print and grow it good, like I should.

I want to thank all of you who have stayed by my side along the way and I will reward you all with 5 shrooms! Thank you! :cheers:


Edited by krock (08/10/20 03:05 PM)


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InvisiblecoAsTal
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
    #26873516 - 08/10/20 02:52 PM (3 years, 5 months ago)

Quote:

krock said:
However I am not going to give up on growing cubes. I will acquire a legit print and grow it good, like I should.

I want to thank all of you who have stayed by my side along the way and I will reward you all with 5 shrooms! Thank you! :cheers:




:yess:


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

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InvisiblePastywhyteMDiscord
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: coAsTal] * 1
    #26873519 - 08/10/20 02:55 PM (3 years, 5 months ago)

Kudos to the OP for sticking with things and learning from the experience. Rated 5 :mushroom2:


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Offlineredhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: Pastywhyte] * 1
    #26873528 - 08/10/20 03:02 PM (3 years, 5 months ago)

I too rated you OP. Btw, I think you should head to the trade forum and see if there are giveaways. Im sure you will find someone to send you prints within the week (may take longer to receive them). You're a nice guys, and gave it your best: sure someone will help!


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