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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat]
#26802816 - 07/04/20 05:08 AM (3 years, 6 months ago) |
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Wow, I just woke up and all of the T2 plates already broke through the hot pour layer! I will transfer them ASAP.

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why isn't your agar clear? Whats your recipe?
My agar is clear. It is just photographed on a black background. Recipe: 10g Malt extract 10g agar agar 500 ml water. Very standard MEA.
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redhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26802959 - 07/04/20 07:32 AM (3 years, 6 months ago) |
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Ah I guess it was condensation then. IMO Its looking good mate. Looks like you probably have some myc. The step is now to get a couple of clean looking plates aimed for grains
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat]
#26803299 - 07/04/20 10:23 AM (3 years, 6 months ago) |
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Sweet! I really hope they are the mycelium I am looking for 
I was thinking of making 5 brf pucks in half pint jars so that I could do a test run from the T3 plates. Do you think it's easier to test them on grain?
Then take a print from whatever fruits to restart the gene pool before putting it in a shoebox?
I am unsure if the genetics I have currently are any good, would this be a good idea? Or should I just try growing what I have?
I could also clone one of the fruits and use that as a base?
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redhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26803337 - 07/04/20 10:46 AM (3 years, 6 months ago) |
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Quote:
krock said: Sweet! I really hope they are the mycelium I am looking for 
I was thinking of making 5 brf pucks in half pint jars so that I could do a test run from the T3 plates. Do you think it's easier to test them on grain?
Then take a print from whatever fruits to restart the gene pool before putting it in a shoebox?
I am unsure if the genetics I have currently are any good, would this be a good idea? Or should I just try growing what I have?
I could also clone one of the fruits and use that as a base?
Fo BRF you would need to wait longer and how are you doing to inoculate BRF with agar? (need syringe no?)
As long as you "save" a clean plate you dont have to worry about not doing a shoebox. You have many options once you get a few clean plates. You could take it to grains (depending on the amount of agar, temp, cleanliness) and have fully colonized jars within 2 weeks (I just had jars finish by 8 days). And then just do a few separate boxes and you'll get shrooms to clone, to print, and to eat 
You could also top fruit your grains if you want quick shrooms and your jars have some contams still. But either way, get yourself the nice and clean plates first.
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat]
#26803451 - 07/04/20 11:52 AM (3 years, 6 months ago) |
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I thought I could inoculate BRF with agar, the same way as with grains. I searched a bit and someone suggest squirting a small agar sample with a sterile water syringe to BRF.
But I see your point. BRF is probably slower. I think doing grains sounds like a good idea once my plates are very clean! I have all the necessary supplies. Thanks for the input 
I'm really looking forward to testing the cultures
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Pastywhyte
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26803478 - 07/04/20 12:06 PM (3 years, 6 months ago) |
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Looks like the myc on my yeasty spore inoculated plates have over come the yeast and will be in clean territory, enough to get a transfer. I’m gonna transfer soon, will post a pic. Most yeasts are pretty weak and stall in cool temps. Should have some plates ready by T3. Was cool in some spots it looked like the mycelium chose to grown through the agar under the yeast. Then shot up at the edge of the colony. Then helped more mycelium leap the yeast. I wish I took more pics but I’ve been busy.
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redhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: Pastywhyte]
#26803492 - 07/04/20 12:17 PM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: Looks like the myc on my yeasty spore inoculated plates have over come the yeast and will be in clean territory, enough to get a transfer. I’m gonna transfer soon, will post a pic. Most yeasts are pretty weak and stall in cool temps. Should have some plates ready by T3. Was cool in some spots it looked like the mycelium chose to grown through the agar under the yeast. Then shot up at the edge of the colony. Then helped more mycelium leap the yeast. I wish I took more pics but I’ve been busy.
Wow Pasty that sounds pretty awesome actually!
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat]
#26803681 - 07/04/20 02:18 PM (3 years, 6 months ago) |
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Quote:
Looks like the myc on my yeasty spore inoculated plates have over come the yeast and will be in clean territory, enough to get a transfer. I’m gonna transfer soon, will post a pic. Most yeasts are pretty weak and stall in cool temps. Should have some plates ready by T3. Was cool in some spots it looked like the mycelium chose to grown through the agar under the yeast. Then shot up at the edge of the colony. Then helped more mycelium leap the yeast. I wish I took more pics but I’ve been busy.
That sounds really cool! Please post pics when you can
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Pastywhyte
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26803773 - 07/04/20 03:10 PM (3 years, 6 months ago) |
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Here’s the best I can do without firing up the flowhood. Some of the colonies were totally overtaken. Now this yeast isn’t dead, just trapped enough so I can make a transfer.
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: Pastywhyte]
#26805664 - 07/05/20 05:21 PM (3 years, 6 months ago) |
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On the subject of mycelium overcoming yeast; I just noticed that a few of my initial streak plates are exhibiting what looks like mycelium from some of the yeast colonies. Almost like the cube spores germinated inside the yeast colonies. Quite interesting to look at. Does this resemble the initial growth you saw, Pasty?

Here is an update on my T3 transfers I did yesterday from the hot poured T2 plates. I scraped off a thin layer of agar that had surface growth on it.
I had to photograph the plates with back light since the growth is very transparent at the moment (is this something to be concerned of?).
With black background the growth is almost invisible right now:
Here the plates are backlit:
Growth seems cleaner for most plates. Though B2T3X and B2T3Y still look a bit bacterial, not really sure yet.
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26806705 - 07/06/20 11:02 AM (3 years, 6 months ago) |
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Another agar update. T3's have started to be a bit more visible now. Also the bacteria contamination on the B2T3X and B2T3Y has become more obvious now. I guess I need to hot pour them a couple more times before they are clean.
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26810275 - 07/08/20 04:12 AM (3 years, 6 months ago) |
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yo, it is day 4 of my T3 plates and here they are. I think B2T3X2 and B2T3Y2 are starting to look a lot like proper mycelium. What do you guys think?

I'm going to transfer all the T3's to new plates today because I going away for a few days soon. Bigger chance of clean plates if I do another transfer right? I will try out my newly acquired metal straw tool that I bent for better sterility: maybe I can get some nice looking transfers 
Unfortunately I don't think I will be able to do a hot pour today, so that'll be when I'm back again. Also when I come back again I will prep some grain to test grow the non-bacterial plates.
Also here is an update on the T2 plates (day 8):
 Looks like the B2T2X2 and B2T2Y2 are going tomentose? Not sure why, but seems promising in regards to having proper mycelium.
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coAsTal
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26810435 - 07/08/20 06:33 AM (3 years, 6 months ago) |
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Looks like your diligence has paid off!!
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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redhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: coAsTal]
#26811198 - 07/08/20 03:35 PM (3 years, 6 months ago) |
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Dude those T3s look really good. Well done mate. I dont think you really need to keep on transferring, but you have nothing to lose by doing it. Probably will have more rhizo (good) growth with subsequent transfers.
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Pastywhyte
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat]
#26811308 - 07/08/20 04:41 PM (3 years, 6 months ago) |
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My T1 transfers look clean and leaping off great. Yeast can suck it.
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: Pastywhyte]
#26820609 - 07/13/20 07:44 AM (3 years, 6 months ago) |
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Quote:
Looks like your diligence has paid off!!
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Dude those T3s look really good. Well done mate. I dont think you really need to keep on transferring, but you have nothing to lose by doing it. Probably will have more rhizo (good) growth with subsequent transfers.
Thank you guys!!
I just put 7 half pint jars with oat grain into the PC. Excited to test out the plates I have 
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My T1 transfers look clean and leaping off great. Yeast can suck it.
Cool, pasty! I had some of the streak plates that got some kind of similar growth to what you described earlier where myc grows from the colony.
does this look similar to you?
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Pastywhyte
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26820663 - 07/13/20 08:52 AM (3 years, 6 months ago) |
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No those look like just yeast. In mine very obvious rhizomorphs sprung up out of the centre of the colony and reached towards the colony that grew under the yeast and surfaced outside the perimeter. Together they bridged the yeast and trapped it allowing me to get a clean sample from the leading edge.
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redhandmat
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: Pastywhyte]
#26820767 - 07/13/20 10:07 AM (3 years, 6 months ago) |
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Quote:
Pastywhyte said: No those look like just yeast. In mine very obvious rhizomorphs sprung up out of the centre of the colony and reached towards the colony that grew under the yeast and surfaced outside the perimeter. Together they bridged the yeast and trapped it allowing me to get a clean sample from the leading edge.
They were waging war on eachother. I guess with your help the myc won out
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: redhandmat]
#26835702 - 07/21/20 08:58 AM (3 years, 6 months ago) |
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Heya people! Grain jars have been growing for 1 week now. I think the growth looks a bit weird, any comments on these jars?
A4T4: B2T3X2: B2T3Y2: 
For comparison, here is a jar that is not inoculated with anything:
Also the yeast plates I showed earlier have grown more myc kind of like things (is this really how yeast grows?)
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krock
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Re: 4th attempt at cleaning up my yeasty spore solution [Re: krock]
#26846343 - 07/26/20 03:35 PM (3 years, 6 months ago) |
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Update, day 12 half pint jars

I gave the jars a smell before spawning them.
B2T3X2; I shook the jar 5 days ago. The myc didn't really grow much afterwards. When opened, it smelled yeasty. Which is kind of weird since I haven't seen any yeast on the agar plates since the first few transfers. The grains did not stick to each other either.
B2T3Y2; I didn't shake this jar and it is now 100%. When opened smelled slightly yeasty. It came out as a compact grain cake (as you would expect from colonized grain jar). A4T4; It's hard to tell whether this jar is fully colonized or not because of it's grey mycelium. However it looked like each individual grain had some on it. When opened, the jar smells like wild mushrooms picked in the forest. I take that as a good sign.
I decided to spawn all of the jars to their own shoeboxes today with coir. I found some cool 1.5 liter totes at the hardware store, perfect size for a half pint jar of grain. I'm following the SFF shoebox assembly TEK

Fingers crossed that at least one of the shoeboxes produces fruits!
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