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Infiniteshot
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Laminar flow hood filter damage. Hepa me!
#26778817 - 06/24/20 09:36 AM (3 years, 7 months ago) |
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Hey all, just received a hepa filter today. 24x24 x6. When I inboxed it I saw these bends in the aluminum. The fabric hepa doesn’t appear to be torn. I’m still a little worried. Does anyone have any feedback to these photos? Thanks a ton for your time.
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Gan
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26778835 - 06/24/20 09:44 AM (3 years, 7 months ago) |
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For slight damage, you can bend them back very gently with needle nose pliers or something similar. However, with that kind of damage I'd ask for a refund or an exchange for another filter that isn't damaged. You might be fine just putting it back straight but you wouldn't know until you build you hood
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Gan]
#26778855 - 06/24/20 09:53 AM (3 years, 7 months ago) |
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Thanks for the quick response. Another filter is on its way. Basically instantaneous great customer service. I’ve built the box and it fits in fine. From what I’ve been reading on this site is that tearing of the medium or separation of the medium from the box are the biggest issues. In a few days I’ll have two on hand and wasn’t sure whether to toss this one or to try and salvage it. I think I’ll install the filter into the box and see how it works. I have a 2.5 air quality monitor and maybe that will give some info. I made the box so that filters can be replaced easily and in a way the back can be removed to check any internal issues.
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Sockadin



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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26778956 - 06/24/20 10:22 AM (3 years, 7 months ago) |
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You can fix that one. Det a long dowel rod 1/4 from Hardware store and some needle nose. You should see my FH filter after all these years. It's beat to hell. Just straighten for laminar flow.
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RoscoeReturns
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26778976 - 06/24/20 10:29 AM (3 years, 7 months ago) |
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I wouldn’t use that filter. I’ve tested hepa filters for ten years, and any time one looked that bad, it leaked. You may not see a tear on the surface, but the filter is 6” deep. There is likely damage to the filter, and to be honest you might not be able to see it even if it was on the surface. If it was just a small area, I might try to fix it with some RTV silicone, but you would be covering a significant portion of your filter. Without the ability to leak test after install, that is a gamble I wouldn’t want to risk.
Please don’t stick anything into the filter, like a dowel or needle nose. Those aluminum separators can be a little bent up and not hurt anything. You’re more likely to do some damage to the filter while trying to repair them.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Sockadin]
#26778982 - 06/24/20 10:31 AM (3 years, 7 months ago) |
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Quote:
Sockadin said: You can fix that one. Det a long dowel rod 1/4 from Hardware store and some needle nose. You should see my FH filter after all these years. It's beat to hell. Just straighten for laminar flow.
Thanks a bunch. I’ll give it a go. I can’t believe how poorly this unit was packaged. Just a basic box. I’ll get on the repair today.
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Sockadin



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Re: Laminar flow hood filter damage. Hepa me! [Re: RoscoeReturns]
#26778985 - 06/24/20 10:31 AM (3 years, 7 months ago) |
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How do you test a Hepa for leaks? I'm interested to try this on mine.
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El Chupacabra
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Re: Laminar flow hood filter damage. Hepa me! [Re: Sockadin]
#26778990 - 06/24/20 10:33 AM (3 years, 7 months ago) |
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If they don't require the damaged one to be returned then it looks like you can make 2 now, or have a replacement hepa. It looks like the damage is just cosmetic just bent aluminum but you can be sure of it by leaving open agar plates in front of your running LFH.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26779009 - 06/24/20 10:38 AM (3 years, 7 months ago) |
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Quote:
El Chupacabra said: If they don't require the damaged one to be returned then it looks like you can make 2 now, or have a replacement hepa. It looks like the damage is just cosmetic just bent aluminum but you can be sure of it by leaving open agar plates in front of your running LFH.
I’m not required to send this one back. The manufacturer says it’s beat to just replace it without all the hassle. (Live good customer care).
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: RoscoeReturns]
#26779012 - 06/24/20 10:40 AM (3 years, 7 months ago) |
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Quote:
RoscoeReturns said: I wouldn’t use that filter. I’ve tested hepa filters for ten years, and any time one looked that bad, it leaked. You may not see a tear on the surface, but the filter is 6” deep. There is likely damage to the filter, and to be honest you might not be able to see it even if it was on the surface. If it was just a small area, I might try to fix it with some RTV silicone, but you would be covering a significant portion of your filter. Without the ability to leak test after install, that is a gamble I wouldn’t want to risk.
Please don’t stick anything into the filter, like a dowel or needle nose. Those aluminum separators can be a little bent up and not hurt anything. You’re more likely to do some damage to the filter while trying to repair them.
Quote:
RoscoeReturns said: I wouldn’t use that filter. I’ve tested hepa filters for ten years, and any time one looked that bad, it leaked. You may not see a tear on the surface, but the filter is 6” deep. There is likely damage to the filter, and to be honest you might not be able to see it even if it was on the surface. If it was just a small area, I might try to fix it with some RTV silicone, but you would be covering a significant portion of your filter. Without the ability to leak test after install, that is a gamble I wouldn’t want to risk.
Please don’t stick anything into the filter, like a dowel or needle nose. Those aluminum separators can be a little bent up and not hurt anything. You’re more likely to do some damage to the filter while trying to repair them.
Hey there. How would you recommend I test the filter? I have an anemometer for air flow and in theory I could inoculate a jar or expose some afar and see what happens. What’s your testing method?
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Sockadin



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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26779013 - 06/24/20 10:41 AM (3 years, 7 months ago) |
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Hey man if he said don't send it back than it is probably better not to use like Rosco said. I'm not a FH expert, now I'm thinking it might be time to get a new one my self. Mine is 5-7 years old. I can't remember.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Sockadin]
#26779018 - 06/24/20 10:44 AM (3 years, 7 months ago) |
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Bogus.
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El Chupacabra
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Re: Laminar flow hood filter damage. Hepa me! [Re: Sockadin]
#26779024 - 06/24/20 10:46 AM (3 years, 7 months ago) |
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Anemometers are pretty pointless TBH. You should just run a live test by placing clean agar plates in front of it, take the lids off and leave them off for a while. If you end up with contamed plates then you know that you don't have good laminar flow. The only thing I'm unsure about is how long you should leave them uncovered. Someone will chime in I'm sure
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26779026 - 06/24/20 10:47 AM (3 years, 7 months ago) |
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Thanks a bunch. Really appreciate it.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26779028 - 06/24/20 10:49 AM (3 years, 7 months ago) |
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Since this is my first flow hood and I can’t make agar plates to rest it before the replacement arrives. Could I just make a few jars of grain that I know for a fact are sterile and then open those up in front of it for the test ?
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Sockadin



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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26779034 - 06/24/20 10:49 AM (3 years, 7 months ago) |
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That's not testing laminar flow leakage. That's just testing percentage of contamination. I'm really curious how they test laminar flow in the industry. Like a dye of some kind pumped into the back of the fan? Something that won't clog the filter? I really have no idea.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Sockadin]
#26779035 - 06/24/20 10:49 AM (3 years, 7 months ago) |
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Its easy to test if you have a particle counter or hot wire anemometer, sometimes you can even feel the breeze with your hands or face. That filter is totally fucked I guarantee it. This is why flowhood filters shouldn't be sent through UPS or FEDEX and only through a freight carrier on a pallet. They are way too fragile for the treatment they get going through those systems. If any of you have worked at UPS/FEDEX you know what I'm talking about. Goddamn Undertaker throws your filter off the top of a hell in a cell and it plummets 16 feet through an announcers table shit.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26779045 - 06/24/20 10:52 AM (3 years, 7 months ago) |
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Thanks man, I won’t chance it. I love your avatar! When Dwight throws that dummy’s face on his own I couldn’t stop laughing. That whole episode is awesome.
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Pastywhyte
Say hello to my little friend



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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26779250 - 06/24/20 11:55 AM (3 years, 7 months ago) |
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I dunno that looks minor to me. Most big labs are not replacing filters everytime someone dents on or jabs a scalpel into it. It’s called a little silicone and it’s good to go. My flowhood had gotten a few small tears and I siliconed them up and it works fine. All my agar comes out fine with it. I wouldn’t even sweat those dents.
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gizmo1


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Re: Laminar flow hood filter damage. Hepa me! [Re: Pastywhyte]
#26779272 - 06/24/20 12:10 PM (3 years, 7 months ago) |
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Where are you ordering from that lets you keep the old filter.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: gizmo1]
#26779303 - 06/24/20 12:29 PM (3 years, 7 months ago) |
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I’m super paranoid and I feel like a digital “paper trail” gets me closer to trouble than I’d like. PM me and we’ll rap a little. I’ll give you the lowdown.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Pastywhyte]
#26779331 - 06/24/20 12:37 PM (3 years, 7 months ago) |
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I’m thinking I’ll run a sleeve of dishes and see what happens. I’ve got a few tubs that should be done in a few weeks and I would think that my dishes would be contaminated by the time I’m ready to try and clone my favorite fruit. Worst case scenario I’m out dishes and agar right?
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RoscoeReturns
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Re: Laminar flow hood filter damage. Hepa me! [Re: Sockadin]
#26779405 - 06/24/20 01:20 PM (3 years, 7 months ago) |
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Quote:
Sockadin said: How do you test a Hepa for leaks? I'm interested to try this on mine.
We tested them by introducing DOP aerosol into the blower, and scanning the filter with a photometer. That was leak testing only. It’s not something you can DIY. It takes some specialized and expensive equipment.
When certifying LF or BSC hoods, we also took air velocity measurements with a hot wire anemometer across the face of the filter, particle counts at different places on the work surface, and introduced smoke to visualize airflow. On some hoods we would also use a balometer to measure air volume.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: RoscoeReturns]
#26779411 - 06/24/20 01:21 PM (3 years, 7 months ago) |
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I just looked at one piece of the equipment required to get into testing hoods...jeez Louise. That stuffs pricey. I’ll leave testing to the pros
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26830690 - 07/18/20 12:27 PM (3 years, 6 months ago) |
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Ok. Update. New filter arrived and box is built. The anemometer I have is totally whack. It’s reading 153 and then only jumps to 28 fpm. So I’m trying the lighter gauge method. How’s this look? Thanks for your time.

Edited by Infiniteshot (07/18/20 12:32 PM)
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The Ole Airstream
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26830707 - 07/18/20 12:40 PM (3 years, 6 months ago) |
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My 24x24 hood blows the lighter out even from 6” away
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southbounpachyderm
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26830717 - 07/18/20 12:50 PM (3 years, 6 months ago) |
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Quote:
Infiniteshot said: Ok. Update. New filter arrived and box is built. The anemometer I have is totally whack. It’s reading 153 and then only jumps to 28 fpm. So I’m trying the lighter gauge method. How’s this look? Thanks for your time.


That doesn't look like you're pushing enough air. Probably gonna need to get a new blower to do effective work in front of that thing.
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Gan
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Flame should be 45-60 degrees approximately 6 inches from the face of the filter
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Infiniteshot
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Quote:
southbounpachyderm said:
Quote:
Infiniteshot said: Ok. Update. New filter arrived and box is built. The anemometer I have is totally whack. It’s reading 153 and then only jumps to 28 fpm. So I’m trying the lighter gauge method. How’s this look? Thanks for your time.


That doesn't look like you're pushing enough air. Probably gonna need to get a new blower to do effective work in front of that thing.
Ok awesome. I had the prefilter tripled up. It’s a cut to fit fabric and I thought the measurement for the 45-60 degrees was at the face of the filter. I removed two of the three layers and here’s the result at 6”. You can see my square down below.
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southbounpachyderm
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26830789 - 07/18/20 01:29 PM (3 years, 6 months ago) |
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Thats looking better but I still would suggest you get a higher powered blower. As mentioned by someone above my lighter is non operational within 6in and looks like yours at around a foot from flowhoods face
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Infiniteshot
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Wild. I did all the calced for the blower and the blower is at about 50cfm more than the project calced at.
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Gan
Wielder of Narya



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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26830853 - 07/18/20 02:05 PM (3 years, 6 months ago) |
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Ideally, the flow shouldn't be so strong that it blows out a lighter 6 inches from the filter. The flame being 45-60 (honestly up to almost 90 degrees isn't a problem most the time) degrees around 6 inches from the filter face is what you want. In a perfect world, you would use an anemometer, but one that is sensitive enough to be accurate at our low flow rates is rather expensive.
OP I cant quite tell the angle of the flame from the angle you took the picture, but if it's following the above criteria, then there's no need for a new blower. Are you using 1 prefilter?
Edited by Gan (07/18/20 02:05 PM)
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southbounpachyderm
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26830892 - 07/18/20 02:23 PM (3 years, 6 months ago) |
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Quote:
Infiniteshot said: Wild. I did all the calced for the blower and the blower is at about 50cfm more than the project calced at.
Are you sure there aren't any leaks around the sides of the filter? could just be your prefilter material. Should be fine tho. I just like to have mine a little overpowered so I can work further from the face '
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Gan]
#26830911 - 07/18/20 02:34 PM (3 years, 6 months ago) |
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Yes. I dropped it down to one filter. I was also thinking that I could run it with the prefilter for an hour before I work and then yank the prefilter off In all reality I thinkk it’s fine taking into consideration the bend of the flame and the fact that I’m pretty good at math. My original calcs used the proper formula and filter. I’ll send a better picture of the flame bend when I get home. I’m trying to navigate a world where nobody understands social distancing traffic patterns.
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Edited by Infiniteshot (07/18/20 03:52 PM)
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inski
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26831059 - 07/18/20 03:55 PM (3 years, 6 months ago) |
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Quote:
Infiniteshot said: Ok. Update. New filter arrived and box is built. The anemometer I have is totally whack. It’s reading 153 and then only jumps to 28 fpm. So I’m trying the lighter gauge method. How’s this look? Thanks for your time.


If this is how your flame looks at that distance over the entire face of your filter it's working very well and producing laminar flow, if you have problems with contamination it will be due to improper sterile technique, if proper techniques are used and there is still contamination the filter is at fault.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: inski]
#26831090 - 07/18/20 04:10 PM (3 years, 6 months ago) |
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The flame is bent at the angle in the photo across the whole face of the filter. I can’t find any zones of turbulence. The only turbulence I come across is while moving the lighter. I have an anemometer and it’s reading 153 fpm. I don’t trust it as it’s a cheap one and it jumps between 28fpm and 153. The 28 and the 153fpm readings are taken at a place where the lighter has a nice steady bend to the flame. This leads me to believe I should discount the anemometer. Thanks a ton.
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inski
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26831101 - 07/18/20 04:19 PM (3 years, 6 months ago) |
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It looks good to me and you are right, none of the cheap anemometers are any good for this application
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: inski]
#26831159 - 07/18/20 04:53 PM (3 years, 6 months ago) |
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Thanks. I appreciate your time and perspective. Have a great weekend.
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Gan
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26831168 - 07/18/20 04:58 PM (3 years, 6 months ago) |
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Also, proof is in the pudding. Throw a few text plates in front and see if they stay clean after letting them sit for a week or so
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Gan]
#26831323 - 07/18/20 06:30 PM (3 years, 6 months ago) |
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That was going to be my move. Not much loss in some agar and dishes. Thanks a bunch
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Bsdgaou

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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26831689 - 07/19/20 12:07 AM (3 years, 6 months ago) |
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I found one spot in my HEPA which makes lighter fire sparkling. I guess this is not called turbulence, because it would be on whole filter. Any idea?
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Bsdgaou]
#26832099 - 07/19/20 07:53 AM (3 years, 6 months ago) |
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I really have no idea. I’ve basically stitched together my idea of what laminar flow should look like based on my past experiences with how fire behaves in other situations. When trying to light something in the wind (camp fire, cigarette, firecracker), I’ve noticed an erratic inconsistent flame and when I see nice seeing smoke blowing in a nice breeze it keeps its structure and there are no breaks or eddies. That’s the basics I understand. I’m not sure about sparkling.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26833914 - 07/20/20 09:40 AM (3 years, 6 months ago) |
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Ok. I did 25 plates last night and things appeared to go well. Some of my dishes had lots of condensation and that’s another issue I’ll have to work out on its own (temp). So how long do you think it should take to see any growth if the hepa isn’t blowing clean? Thanks for your time and everyone have a great week.
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El Chupacabra
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26834054 - 07/20/20 11:23 AM (3 years, 6 months ago) |
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You'll know pretty fast, You should likely see something on those petri dishes within the first week if they are gonna contam at all. You can set a few of those petri dishes aside and uncover them in open air to intentionally contaminate them, leave the lid off for 30 minutes or so then you would have an idea to compare it against. That's what I did and those plates showed me what bacteria looks like and how fast it spreads meanwhile the others stayed perfect looking for months.
I don't know how others do it here, but after I pour up a fresh batch of plates I end up putting them aside for a week before they get used. That way if I accidentally screwed up somehow with my technique then I'll see like a bacterial ring or something around the edges and just toss it. (and that may even be silly because I don't think I've ever actually had to toss any plates that were contamd after pouring) Someone may pop in and call me a dumb bitch for doing it that way but it's worked great for me. It also helps to get rid of that little tiny bit of condensation in the top plate or two. By the way you want to pour that agar somewhere between 116° and about 120°... Makes nice looking plates. I pour as soon as it hits 120 and they always look nice.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26834292 - 07/20/20 01:29 PM (3 years, 6 months ago) |
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Thanks so much. I really appreciate all the tips. I have one plate opened and ready to be disgusting.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26834400 - 07/20/20 02:41 PM (3 years, 6 months ago) |
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2 weeks no contams to verify
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26834606 - 07/20/20 05:00 PM (3 years, 6 months ago) |
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Awesome. I’m pretty much using this round of plates to check the quality of my flow hood. If it works I’ve got a ton of good stuff to get started
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26842947 - 07/24/20 03:40 PM (3 years, 6 months ago) |
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Ok. So I did these about 6 days ago. Out of twenty five trays I exposed one to unfiltered air. All the other 24 are clean. I’m going to give it another week to see what happens. I also ordered a new hot wire anemometer.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26843060 - 07/24/20 04:53 PM (3 years, 6 months ago) |
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Very nice so far then
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26843092 - 07/24/20 05:08 PM (3 years, 6 months ago) |
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Thanks. So far so good. No activity in all others and that has me optimistic. I’ll know more in a week or so. Have a great weekend.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26848769 - 07/27/20 08:30 PM (3 years, 5 months ago) |
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So the ones I did and kept sealed are all still clean. No contamination. The one I let sit out in the house is a damn rainbow of yuck. What the hell is the pink stuff?
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26850445 - 07/28/20 06:21 PM (3 years, 5 months ago) |
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Ok. Here’s a dish I opened up to the world. It looks disgusting and I hate that pink jazz going on.
the other dish is where I tried to take some genetic material from a fruit I had and it appears to be working the correct way. There’s for sure some mold in there. from what I understand a genetic grab usually needs to be cleaned up until you have a clean sample. Here are some photos and I look forward to some feedback. Have a great evening.
Edited by Infiniteshot (07/28/20 06:30 PM)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26850502 - 07/28/20 06:53 PM (3 years, 5 months ago) |
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or it could be cobweb mold? who knows?
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Edited by Infiniteshot (07/28/20 07:00 PM)
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26850750 - 07/28/20 08:55 PM (3 years, 5 months ago) |
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Quote:
El Chupacabra said: You'll know pretty fast, You should likely see something on those petri dishes within the first week if they are gonna contam at all. You can set a few of those petri dishes aside and uncover them in open air to intentionally contaminate them, leave the lid off for 30 minutes or so then you would have an idea to compare it against. That's what I did and those plates showed me what bacteria looks like and how fast it spreads meanwhile the others stayed perfect looking for months.
ok, so I did that and as you can see I clearly have an environment for nasty stuff to grow. the plates I did and have separated from the ones I opened are all still pristine. except for the one I threw some pieces of a fruit into. I tried using an inoculation loop and that experience just sucked ass. no cutting edge to get into the interior flesh. I know I dragged some crap with me when doing it. im really excited about this phase of my learning as I think the hood is working and now all I have to do is learn and adjust my procedures. really a great time. thanks for your input.
I don't know how others do it here, but after I pour up a fresh batch of plates I end up putting them aside for a week before they get used. That way if I accidentally screwed up somehow with my technique then I'll see like a bacterial ring or something around the edges and just toss it. (and that may even be silly because I don't think I've ever actually had to toss any plates that were contamd after pouring) Someone may pop in and call me a dumb bitch for doing it that way but it's worked great for me. It also helps to get rid of that little tiny bit of condensation in the top plate or two. By the way you want to pour that agar somewhere between 116° and about 120°... Makes nice looking plates. I pour as soon as it hits 120 and they always look nice.
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26850825 - 07/28/20 09:57 PM (3 years, 5 months ago) |
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It looks so faint and odd that I think i'd wait it out personally, just to see if anything comes out that better resembles mushroom myc. Doesn't look to great but I guess time will tell. Is that tissue from a cubensis? Is the brown spots sediment that was in the agar? Also when did that clone tissue plate happen?
(definitely label everything with good necessary information)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26850845 - 07/28/20 10:12 PM (3 years, 5 months ago) |
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Yes, the brown was from a fruit that had released spores. It was the only thing I had going so I gave it a go. I totally understand waiting to see where it goes. I really like the idea of logging what things look like at specific stages. That being said I used that fruit on Thursday of last week. I’ve kept all my notes in a spreadsheet and as soon as I do something new I’ll start on the dishes. I should be doing that right now. Good call. I’m really just doing a bunch of trial and error at the moment. Hopefully I’ll get my footing solid on this next tool of the hobby. Thanks a ton.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26851128 - 07/29/20 05:20 AM (3 years, 5 months ago) |
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Sorry man that "genetic grab" aka clone is not good, it's a contam for sure.
That is not what it should look like when you take a clone. The bright white should continue and grow not change to a light grey mycelium. That is some white mold or totally botched hijacked mycelium at the best..
Brown sediment is troubling, if not for anything else it is visually distracting from seeing contams. That is not from a fruit dropping spores (?? did i read that right as your explanation?) Did you add nutritional yeast to your agar? Maybe use a stick blender on your agar while its simmering to get a good mix of your nutes.
Your clone technique will take practice. Tear from base through cap, take an inner tissue sample. Keep fingers out of the way.
Funny enough a still air box is more reliable for taking clones than a flowhood. Because the airflow can potentially blow anything on the outside surface of the fruit to the inner virgin tissue when you tear it open. You can see how still air would be best for transferring from something with possible dirty spores on it.
Edited by sandman420 (07/29/20 05:28 AM)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26851218 - 07/29/20 06:59 AM (3 years, 5 months ago) |
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Yeah. It was a real shit show. I used alcohol to clean the fruit and there were spores already there so it became a bit of a oil slick. From there it just got dumb. Keep on keepin on ya know. The good thing is that my agar plates are not contaminating. Oh and yeah. That brown junk is for sure poor agar plate construction. Looks like silt and crap.
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26851224 - 07/29/20 07:04 AM (3 years, 5 months ago) |
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Do not use anything to clean the fruits IMO. It soaks right through the tissue like a sponge and your transfers can die like this one did. Was it murdered by the mold or murdered by being wet with alcohol? I'm calling Matlock...
Edited by sandman420 (07/29/20 07:05 AM)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26851263 - 07/29/20 07:24 AM (3 years, 5 months ago) |
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It was a god damned sludge bath. Clues everywhere. I say I get the murder she wrote gal in on it. Angela
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26851304 - 07/29/20 07:47 AM (3 years, 5 months ago) |
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This whole thing gave me a raging hard clue lol. Clean plates to play with is definitely the silver lining here (play a little less messy next time) Watch some videos of tissue cloning and spore printing and shit like that and keep on documenting and reading here and asking questions and you'll be dehydrating big ole yields in no time.
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26851324 - 07/29/20 07:56 AM (3 years, 5 months ago) |
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Totally. My plates appear to be clean except for my poor agar mixing. The plates I did ten days ago and left sealed are all clean and non contaminated. I’ve got some projects going that should produce in the next 5 weeks or so. I’ll get to some experiments at that point. It will give me plenty of time to eyeball those plates and make sure they’re perfect. Thanks for the input.
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26851723 - 07/29/20 12:30 PM (3 years, 5 months ago) |
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And one more thing...I ordered a hot wire anemometer and got it up to the face of the hepa filter for a reading. It was reading 104-112. Sometimes it dropped to 96. It’s substantially more sensitive than the vane anemometer. That reading held at 6 inches out and then fell off. When reading FPM , what distance away from the filter do you advise should be used ?
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26852024 - 07/29/20 02:39 PM (3 years, 5 months ago) |
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~6" because that where you will be working.
Don't work directly next to or close to the surface of the filter, the air needs room to make a laminar flow.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26852231 - 07/29/20 04:29 PM (3 years, 5 months ago) |
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More info. So I pulled up the anemometer and started testing at 6” distance from the face. The readings across the bottom are at about 115 FPM (all readings are FPM) at the middle they’re about 90 , and towards the top it hits 110. This is with the prefilter on and that could be removed. Everything increases by about 10 when removed. The question is would it be better to have the middle a bit low or to keep prefilter off while working and have everything high? Thanks again.
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26852278 - 07/29/20 04:45 PM (3 years, 5 months ago) |
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It sounds like your flowhood is functioning about as good as a homemade DIY flowhood will function. 90-110 is a good range.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26852309 - 07/29/20 04:58 PM (3 years, 5 months ago) |
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Thanks for the help. Have a great day
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26859069 - 08/02/20 02:36 PM (3 years, 5 months ago) |
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Here’s another I did last week. I feel a bit better about this one.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26859106 - 08/02/20 03:04 PM (3 years, 5 months ago) |
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protip: cut your parafilm in half lengthwise to get twice the parafilm and none of the obstruction.
You only need to seal that little gap at the bottom.
Looks good man.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26859112 - 08/02/20 03:07 PM (3 years, 5 months ago) |
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Thanks. Am I correct in thinking that on the left side it looks a little “wispy” and needs to be isolated?
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26859117 - 08/02/20 03:09 PM (3 years, 5 months ago) |
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yea keep going for isolation id take a bunch of transfers around 2-3-4 o'clock
Edited by sandman420 (08/02/20 03:10 PM)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26864969 - 08/05/20 04:34 PM (3 years, 5 months ago) |
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Hey there. So I isolated these sections of the previous plates. This is my final result. Should I keep isolating pieces or does this look cleaned up? Thanks for your time.
-------------------- rad
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