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Bsdgaou

Registered: 02/20/19
Posts: 340
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26831689 - 07/19/20 12:07 AM (3 years, 6 months ago) |
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I found one spot in my HEPA which makes lighter fire sparkling. I guess this is not called turbulence, because it would be on whole filter. Any idea?
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Bsdgaou]
#26832099 - 07/19/20 07:53 AM (3 years, 6 months ago) |
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I really have no idea. I’ve basically stitched together my idea of what laminar flow should look like based on my past experiences with how fire behaves in other situations. When trying to light something in the wind (camp fire, cigarette, firecracker), I’ve noticed an erratic inconsistent flame and when I see nice seeing smoke blowing in a nice breeze it keeps its structure and there are no breaks or eddies. That’s the basics I understand. I’m not sure about sparkling.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26833914 - 07/20/20 09:40 AM (3 years, 6 months ago) |
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Ok. I did 25 plates last night and things appeared to go well. Some of my dishes had lots of condensation and that’s another issue I’ll have to work out on its own (temp). So how long do you think it should take to see any growth if the hepa isn’t blowing clean? Thanks for your time and everyone have a great week.
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El Chupacabra
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26834054 - 07/20/20 11:23 AM (3 years, 6 months ago) |
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You'll know pretty fast, You should likely see something on those petri dishes within the first week if they are gonna contam at all. You can set a few of those petri dishes aside and uncover them in open air to intentionally contaminate them, leave the lid off for 30 minutes or so then you would have an idea to compare it against. That's what I did and those plates showed me what bacteria looks like and how fast it spreads meanwhile the others stayed perfect looking for months.
I don't know how others do it here, but after I pour up a fresh batch of plates I end up putting them aside for a week before they get used. That way if I accidentally screwed up somehow with my technique then I'll see like a bacterial ring or something around the edges and just toss it. (and that may even be silly because I don't think I've ever actually had to toss any plates that were contamd after pouring) Someone may pop in and call me a dumb bitch for doing it that way but it's worked great for me. It also helps to get rid of that little tiny bit of condensation in the top plate or two. By the way you want to pour that agar somewhere between 116° and about 120°... Makes nice looking plates. I pour as soon as it hits 120 and they always look nice.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26834292 - 07/20/20 01:29 PM (3 years, 6 months ago) |
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Thanks so much. I really appreciate all the tips. I have one plate opened and ready to be disgusting.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26834400 - 07/20/20 02:41 PM (3 years, 6 months ago) |
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2 weeks no contams to verify
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26834606 - 07/20/20 05:00 PM (3 years, 6 months ago) |
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Awesome. I’m pretty much using this round of plates to check the quality of my flow hood. If it works I’ve got a ton of good stuff to get started
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26842947 - 07/24/20 03:40 PM (3 years, 6 months ago) |
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Ok. So I did these about 6 days ago. Out of twenty five trays I exposed one to unfiltered air. All the other 24 are clean. I’m going to give it another week to see what happens. I also ordered a new hot wire anemometer.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26843060 - 07/24/20 04:53 PM (3 years, 6 months ago) |
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Very nice so far then
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26843092 - 07/24/20 05:08 PM (3 years, 6 months ago) |
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Thanks. So far so good. No activity in all others and that has me optimistic. I’ll know more in a week or so. Have a great weekend.
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26848769 - 07/27/20 08:30 PM (3 years, 5 months ago) |
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So the ones I did and kept sealed are all still clean. No contamination. The one I let sit out in the house is a damn rainbow of yuck. What the hell is the pink stuff?
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26850445 - 07/28/20 06:21 PM (3 years, 5 months ago) |
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Ok. Here’s a dish I opened up to the world. It looks disgusting and I hate that pink jazz going on.
the other dish is where I tried to take some genetic material from a fruit I had and it appears to be working the correct way. There’s for sure some mold in there. from what I understand a genetic grab usually needs to be cleaned up until you have a clean sample. Here are some photos and I look forward to some feedback. Have a great evening.
Edited by Infiniteshot (07/28/20 06:30 PM)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26850502 - 07/28/20 06:53 PM (3 years, 5 months ago) |
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or it could be cobweb mold? who knows?
-------------------- rad
Edited by Infiniteshot (07/28/20 07:00 PM)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26850750 - 07/28/20 08:55 PM (3 years, 5 months ago) |
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Quote:
El Chupacabra said: You'll know pretty fast, You should likely see something on those petri dishes within the first week if they are gonna contam at all. You can set a few of those petri dishes aside and uncover them in open air to intentionally contaminate them, leave the lid off for 30 minutes or so then you would have an idea to compare it against. That's what I did and those plates showed me what bacteria looks like and how fast it spreads meanwhile the others stayed perfect looking for months.
ok, so I did that and as you can see I clearly have an environment for nasty stuff to grow. the plates I did and have separated from the ones I opened are all still pristine. except for the one I threw some pieces of a fruit into. I tried using an inoculation loop and that experience just sucked ass. no cutting edge to get into the interior flesh. I know I dragged some crap with me when doing it. im really excited about this phase of my learning as I think the hood is working and now all I have to do is learn and adjust my procedures. really a great time. thanks for your input.
I don't know how others do it here, but after I pour up a fresh batch of plates I end up putting them aside for a week before they get used. That way if I accidentally screwed up somehow with my technique then I'll see like a bacterial ring or something around the edges and just toss it. (and that may even be silly because I don't think I've ever actually had to toss any plates that were contamd after pouring) Someone may pop in and call me a dumb bitch for doing it that way but it's worked great for me. It also helps to get rid of that little tiny bit of condensation in the top plate or two. By the way you want to pour that agar somewhere between 116° and about 120°... Makes nice looking plates. I pour as soon as it hits 120 and they always look nice.
-------------------- rad
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El Chupacabra
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26850825 - 07/28/20 09:57 PM (3 years, 5 months ago) |
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It looks so faint and odd that I think i'd wait it out personally, just to see if anything comes out that better resembles mushroom myc. Doesn't look to great but I guess time will tell. Is that tissue from a cubensis? Is the brown spots sediment that was in the agar? Also when did that clone tissue plate happen?
(definitely label everything with good necessary information)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26850845 - 07/28/20 10:12 PM (3 years, 5 months ago) |
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Yes, the brown was from a fruit that had released spores. It was the only thing I had going so I gave it a go. I totally understand waiting to see where it goes. I really like the idea of logging what things look like at specific stages. That being said I used that fruit on Thursday of last week. I’ve kept all my notes in a spreadsheet and as soon as I do something new I’ll start on the dishes. I should be doing that right now. Good call. I’m really just doing a bunch of trial and error at the moment. Hopefully I’ll get my footing solid on this next tool of the hobby. Thanks a ton.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26851128 - 07/29/20 05:20 AM (3 years, 5 months ago) |
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Sorry man that "genetic grab" aka clone is not good, it's a contam for sure.
That is not what it should look like when you take a clone. The bright white should continue and grow not change to a light grey mycelium. That is some white mold or totally botched hijacked mycelium at the best..
Brown sediment is troubling, if not for anything else it is visually distracting from seeing contams. That is not from a fruit dropping spores (?? did i read that right as your explanation?) Did you add nutritional yeast to your agar? Maybe use a stick blender on your agar while its simmering to get a good mix of your nutes.
Your clone technique will take practice. Tear from base through cap, take an inner tissue sample. Keep fingers out of the way.
Funny enough a still air box is more reliable for taking clones than a flowhood. Because the airflow can potentially blow anything on the outside surface of the fruit to the inner virgin tissue when you tear it open. You can see how still air would be best for transferring from something with possible dirty spores on it.
Edited by sandman420 (07/29/20 05:28 AM)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26851218 - 07/29/20 06:59 AM (3 years, 5 months ago) |
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Yeah. It was a real shit show. I used alcohol to clean the fruit and there were spores already there so it became a bit of a oil slick. From there it just got dumb. Keep on keepin on ya know. The good thing is that my agar plates are not contaminating. Oh and yeah. That brown junk is for sure poor agar plate construction. Looks like silt and crap.
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sandman420
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26851224 - 07/29/20 07:04 AM (3 years, 5 months ago) |
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Do not use anything to clean the fruits IMO. It soaks right through the tissue like a sponge and your transfers can die like this one did. Was it murdered by the mold or murdered by being wet with alcohol? I'm calling Matlock...
Edited by sandman420 (07/29/20 07:05 AM)
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Infiniteshot
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26851263 - 07/29/20 07:24 AM (3 years, 5 months ago) |
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It was a god damned sludge bath. Clues everywhere. I say I get the murder she wrote gal in on it. Angela
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