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Offlinefiddy
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Registered: 06/03/20
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Last seen: 1 year, 7 months
First time Agar
    #26778125 - 06/23/20 11:32 PM (3 years, 7 months ago)

Hi, first time grower. Grateful for all the time others have spent to educate!

I followed Stro’s agar recipe:

500ml distilled water (1/2 liter)
10g Agar-Agar
10g Malt Extract
1g yeast

I followed BOD’s agar prep tek.

I followed C10’s streaking technique.

I made 40 plates to have some on hand for transfers. I had 2 spore syringes and one spore print. I made two plates from each of the samples using C10’s streaking technique on 6/15, 8 days ago.

I have one plate with a bunch of white dots, one plate with some myc growth, and one plate with a tiny myc growth.

I am at 8 days and I wonder if my agar wasn’t mixed that well. I have tiny specs of what looks like not mixed agar in my plates. Could this be what is causing my stuff to grow slow? I have been keeping the plates at 75 degrees.

Is the one growth large enough to do my first transfer?

Thank you



Edited by fiddy (06/23/20 11:45 PM)


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OfflineFRUITS
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Registered: 05/11/20
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Re: First time Agar [Re: fiddy]
    #26778280 - 06/24/20 01:31 AM (3 years, 7 months ago)

:rocket:
Kudos to you for mixing up your own agar and not getting any bacteria, by the look of things. I’ll actually be giving agar a go for my first time in a few days, however sadly I purchased pre poured plates from Amazon. Not sure but looks like you’re doing pretty well.


Edited by FRUITS (06/24/20 01:34 AM)


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OfflineSoccrates
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Registered: 04/10/19
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Re: First time Agar [Re: FRUITS]
    #26778287 - 06/24/20 01:39 AM (3 years, 7 months ago)

2 things- 1. It can take a full week for them to even germinate. So, no biggie there.
And 2. Less nutrients=faster growth as the mycelium will stretch looking for food, rather than having plenty and no motivation. I might be wrong on this part (from memory, no notes here) , but I think 10g agar and 5g nutrient is more standard. At least around here- for that very reason. Now, aloha medicinals lists some very rich agar recipes, they just won't grow as fast


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OfflineSockadin
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Registered: 01/03/10
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Trusted Cultivator
Re: First time Agar [Re: Soccrates]
    #26778294 - 06/24/20 01:50 AM (3 years, 7 months ago)

Picture 2-4 are all contaminated with bactiera unfortunately. Sorry man.


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Offlinefiddy
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Re: First time Agar [Re: Soccrates]
    #26778881 - 06/24/20 10:02 AM (3 years, 7 months ago)

Quote:

Soccrates said:
2 things- 1. It can take a full week for them to even germinate. So, no biggie there.
And 2. Less nutrients=faster growth as the mycelium will stretch looking for food, rather than having plenty and no motivation. I might be wrong on this part (from memory, no notes here) , but I think 10g agar and 5g nutrient is more standard. At least around here- for that very reason. Now, aloha medicinals lists some very rich agar recipes, they just won't grow as fast




I will have to look into less nutrient, makes sense. Thanks

Quote:

Sockadin said:
Picture 2-4 are all contaminated with bactiera unfortunately. Sorry man.




Sock, Where are you seeing the bacteria?


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