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fiddy
Stranger

Registered: 06/03/20
Posts: 8
Last seen: 1 year, 7 months
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First time Agar
#26778125 - 06/23/20 11:32 PM (3 years, 7 months ago) |
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Hi, first time grower. Grateful for all the time others have spent to educate!
I followed Stro’s agar recipe:
500ml distilled water (1/2 liter) 10g Agar-Agar 10g Malt Extract 1g yeast
I followed BOD’s agar prep tek.
I followed C10’s streaking technique.
I made 40 plates to have some on hand for transfers. I had 2 spore syringes and one spore print. I made two plates from each of the samples using C10’s streaking technique on 6/15, 8 days ago.
I have one plate with a bunch of white dots, one plate with some myc growth, and one plate with a tiny myc growth.
I am at 8 days and I wonder if my agar wasn’t mixed that well. I have tiny specs of what looks like not mixed agar in my plates. Could this be what is causing my stuff to grow slow? I have been keeping the plates at 75 degrees.
Is the one growth large enough to do my first transfer?
Thank you
Edited by fiddy (06/23/20 11:45 PM)
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FRUITS



Registered: 05/11/20
Posts: 50
Loc: United States of America
Last seen: 2 years, 11 months
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Re: First time Agar [Re: fiddy]
#26778280 - 06/24/20 01:31 AM (3 years, 7 months ago) |
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 Kudos to you for mixing up your own agar and not getting any bacteria, by the look of things. I’ll actually be giving agar a go for my first time in a few days, however sadly I purchased pre poured plates from Amazon. Not sure but looks like you’re doing pretty well.
Edited by FRUITS (06/24/20 01:34 AM)
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Soccrates
Stranger
Registered: 04/10/19
Posts: 457
Last seen: 6 months, 15 days
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Re: First time Agar [Re: FRUITS]
#26778287 - 06/24/20 01:39 AM (3 years, 7 months ago) |
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2 things- 1. It can take a full week for them to even germinate. So, no biggie there. And 2. Less nutrients=faster growth as the mycelium will stretch looking for food, rather than having plenty and no motivation. I might be wrong on this part (from memory, no notes here) , but I think 10g agar and 5g nutrient is more standard. At least around here- for that very reason. Now, aloha medicinals lists some very rich agar recipes, they just won't grow as fast
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Picture 2-4 are all contaminated with bactiera unfortunately. Sorry man.
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fiddy
Stranger

Registered: 06/03/20
Posts: 8
Last seen: 1 year, 7 months
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Quote:
Soccrates said: 2 things- 1. It can take a full week for them to even germinate. So, no biggie there. And 2. Less nutrients=faster growth as the mycelium will stretch looking for food, rather than having plenty and no motivation. I might be wrong on this part (from memory, no notes here) , but I think 10g agar and 5g nutrient is more standard. At least around here- for that very reason. Now, aloha medicinals lists some very rich agar recipes, they just won't grow as fast
I will have to look into less nutrient, makes sense. Thanks
Quote:
Sockadin said: Picture 2-4 are all contaminated with bactiera unfortunately. Sorry man.
Sock, Where are you seeing the bacteria?
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