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Offline5vankmajer
ars moriendi


Registered: 06/16/20
Posts: 8
Last seen: 2 years, 3 months
First attempts at agar work for isolation and propagation
    #26777842 - 06/23/20 09:05 PM (3 years, 7 months ago)

Greetings from Argentina. This is my first post, and I've made sure to read all the rules and recommendations, so I hope to be posting in the right forum.

This are my first attempts doing agar work. I prepare it using a MEA recipe I found some time ago on the internets. I use 500ml of tap water, 10g malt extract (syrup), 10g of agar agar, and 2g of nutritional yeast. I sterilize the mix with a pressure cooker for about 45 minutes, let it cool down for over 90 minutes, and then pour it into the cheapest plastic Petri dishes inside a glovebox with the highest care possible (cleaning and disinfection of my workspace [a closed room used solely for this kind of work], of myself, and every tool I use).

Here are some pictures of my plates after various days of colonization at room temperature (~22C / ~72F, but now it's getting colder).

Transfer, 9 days:
https://i.imgur.com/KWupuWg.jpg

Transfer, 9 days:
https://i.imgur.com/kz7zpQE.jpg

Clone apparently fruiting, 8 days ago:
https://i.imgur.com/KxusjHp.jpg

Clone, apparently hating my agar recipe:
https://i.imgur.com/Rd4q934.jpg

Transfer, 3 days:
https://i.imgur.com/UKttSnn.jpg

This is definitely a really bad thing, and I've already tossed it, but I found it so beautiful:

https://i.imgur.com/8DvwoUy.jpg

This was taken at 18 days, and I've stored it in the fridge ever since:
https://i.imgur.com/PJM3yyC.jpg

--

So I feel that growth has been a little slow on this ones. Maybe it's because of the agar recipe I use having too much nutrients. Maybe it's too cold for them to grow faster. I know that agar isn't for expanding mycelium, but nevertheless I feel that I can improve on that recipe for next times.

So far I've made ~60 sterilized agar plates and about 8 have been contaminated. So there's plenty of room for improvement. I plan on getting a "laminar flow hood" and a more sealed and cleaner environment to do this work.

One question I do have is that... it seems that mycelium from ~9 days from cloning from a fruit I got buying from a local seller is already fruiting! That's interesting, because I thought that it could happen, but much, much later (at >3 weeks, maybe?). So yeah, I don't know what to do with that one. Any recommendations?

Thanks in advance.


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Offlinethekickingbird
 User Gallery

Registered: 11/22/19
Posts: 26
Last seen: 2 years, 11 months
Re: First attempts at agar work for isolation and propagation [Re: 5vankmajer]
    #27056672 - 11/25/20 07:15 AM (3 years, 2 months ago)

Hi Mate,

Im from Argentina too, and I really like see other fellow shroomers working on Agar with the same ingredients I have. For the ones how dont know here in Argentina we haven't got the same Agar-Agar you have (I suspect that ours have a nefarius ammount of preservatives), also we don't have Light Malt Extract.

That's because I would like to see further updates in this thread.


Trying to answer your question, I sincerely don't see any fruiting in those images. The "puff"/cotton like formations are normal tomentose growing, product of your MS start point. Other images present more rhizomorphic growing, which you can use to transfer and Isolate.

Hope I see you soon mate,
Kind regards


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