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InvisiblePastywhyteMDiscord
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Cegda]
    #26854105 - 07/30/20 05:51 PM (3 years, 5 months ago)

I can be pretty tough on new people sometimes but I like to also think I’m fair. I remember what it was like when I started, I wanted to do my own thing and not listen to anyone. If it wasn’t for the harsh ass old guard who told it to me straight, I might still be deluding myself. I’m not trying to beef with anyone but, if someone thinks they know better than I do, I want to see proof.


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OfflineCegda
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Pastywhyte]
    #26854125 - 07/30/20 05:58 PM (3 years, 5 months ago)

Man I even said there's no doubt you know more about this subject... I think you took my tyvek comment as me recommending it as a great filter.

I probably sound like you if I'm discussing welding, or blacksmithing... I know about that and tend to sound harsh.

Thats why I don't want to fight with you... shit, I want to learn what you know.

Once I work through the grain I have I'm totally trying your easy af no mod lid shit too


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“What senses do we lack that we cannot see or hear another world all around us?”

-Orange Catholic Bible,
    -from Frank Herbert's Dune


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InvisibleLotKid
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Cegda]
    #26854136 - 07/30/20 06:09 PM (3 years, 5 months ago)

MP tape for lids is awesome. :thumbup:

well... i like it, anyway. :shrug:

:2cents:

:specialralph:

Looks like op has a new "wheel" he's developed.
:neat:


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InvisiblePastywhyteMDiscord
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: LotKid]
    #26854147 - 07/30/20 06:19 PM (3 years, 5 months ago)

I used to do a small square of tyvek held in place with micropore. That is much better than either one or the other as far as grain lid filters goes.


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InvisibleLotKid
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Pastywhyte]
    #26854157 - 07/30/20 06:23 PM (3 years, 5 months ago)

i do 5 small holes in the center of the metal lid... then 3-4 layers of mp tape. quick, cheap, ez and works like a champ for me. :rockon:


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Offlinefightingcherries
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Pastywhyte]
    #26854220 - 07/30/20 06:57 PM (3 years, 5 months ago)

Maybe not an issue up north, but do you ever get fungus gnats?  I have noticed I get them when I have openings in my tubs.  Might be San Diego weather though.


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InvisibleLotKid
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: fightingcherries]
    #26854231 - 07/30/20 07:02 PM (3 years, 5 months ago)

San Diego is a beautiful city. Too damn hott for me tho! :lol:


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OfflineJoBloShow
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Cegda]
    #26854853 - 07/31/20 04:00 AM (3 years, 5 months ago)

Quote:

Cegda said:
He's against a pc.... and I don't see how a 24 hr soak is hard regardless... put it in a bucket the day before you want to pc. This whole hobby is about planning and patience anyway




This.
I always prep my grain the night before and therefore end up with a 24 hour soak. It's easy and convenient. 
PC for 90 min,  zero issues.


--------------------
Following our will and wind we may just go where no one's been,
We'll ride the spiral to the end and may just go where no one's been...



FIND THE BEAUTY IN THE DISSONANCE


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OfflineBrian Jones
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Gastronomicus]
    #26854889 - 07/31/20 05:28 AM (3 years, 5 months ago)

Quote:

checkmatsis said:
Grow Log:

I'm stubborn and tend not to follow directions well, but I always enjoy the path.
Anyway, the intention of this grow log is to document the process I take in cultivating a single large WBS jar to allow multiple G2G transfers that will be utilized to cultivate 5 shoeboxes.

PHASE 1: Skip the soak and speed things up.

Well maybe "skip" is the wrong word, but I will be modifying the process and saving almost a whole day by cooking my WBS in a rice cooker to obtain decent grain hydration.
- Sanitized myself with a few beers.
- Strained out any floaters in the WBS (Might skip this step in the
        future seems messy and unnecessary. Will sunflowers and corn really
        destroy the world? I mean Monsanto, yeah, but come on what's the
        purpose?)
- 2 cups WBS 4 cups water (just like cooking regular old rice -
        keeping it simple. It takes about 50 minutes and there will be some
        WBS left over. So, if you prescribe to waste not, want not, then
        shorten up your measurements to avoid dumping any excess WBS.)
- Once WBS is "cooked," but more like speed soaked,  I put it into
        the single large jar and prepare it for sterilization.

PHASE 2: Steam sterilization as a style choice.

I don't particularly like PC's, so I'm choosing to do one big steam sterilization of the single large jar of WBS. Splitting this process up into fractional steams would probably be better, but I'm trying to keep things moving forward by eliminating any unnecessary waiting time (this may have to be changed depending on contamination).
- WBS is loaded into the single large jar, topped with vermiculite,
        and with some cheesecloth underneath the lid as a substitute for
        polyfill.
- Scrunched up aluminum foil all around the lids edge to prevent any
        moisture from infiltrating the jar during the long steam.
- Placed the jar into a canning pot with an elevated metal platform
          on bottom and brought water to a boil (took about 10 minutes),
          turned down to a simmer, then started the 6 hour timer. (Add pre-
          boiled water as necessary).
- Once the 6 hours was completed I immediately put the WBS in the
        refrigerator for 3 hours (Might've gotten away with 2 hours, but I
        fell asleep, and yes I know that refrigerators are the Mecca of
        contamination and that patience is the key to this hobby, but let's
        just see what happens. Anyway, ideally I will eventually get a
        container that I can sterilize to place the single WBS jar
        inside, then stick it into the refrigerator in its own sterilized
        environment to quickly cool it down).

PHASE 3: Inoculate and wait.

I used a spore syringe that I bought online that is about 2 years old and was unrefrigerated - I know, hate me.
- Sanitized the environment with isopropyl alcohol.
- Sprayed the inside of the SAB with isopropyl alcohol.
- Once the WBS had finished cooling, I gloved up, heated the syringe,
        and inoculated the jar of WBS with the entire spore syringe
        (more spores could potentially increase speed of
        colonization), then I placed tape over the four inoculation holes.
- Told the spores to have beautiful dreams and grow big and strong,
        then placed the jar in the cupboard.

This whole process took me about 10 hours compared to the normal 48 hours or more with all the additional waiting time. If done right this could probably be knocked down to 8 hours which means you could have an inoculated master jar of WBS for G2G transfer much quicker than previously expected.




The title of the post says master jar, which I associate with people who've had a long string of success,  but I'm not getting that impression.

If I were you I would listen to this guy.

Quote:

Gastronomicus said:
While you're waiting for your mold to grow you should read more teks and buy a pressure cooker.




--------------------
"The Rolling Stones will break up over Brian Jones' dead body"    John Lennon

I don't want no commies in my car. No Christians either.

The worst thing about corruption is that it works so well,


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InvisibleLotKid
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Brian Jones]
    #26854926 - 07/31/20 06:12 AM (3 years, 5 months ago)

I dont bother to soak grains anymore. Seems like a waste of time and energy to me.

I use a 25 gal brew kettle to bring 50 lb of rye to a boil. Boil for 5-6 minutes then drain the kettle. The kettle takes about another 10 minutes to drain. Then scoop the grain from the kettle to the strainer. Once dry enough... bag it up.


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Offlinecheckmatsis
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: LotKid]
    #26856261 - 07/31/20 09:05 PM (3 years, 5 months ago)

@Pastywhyte It might not be as strong as rhizomorphic mycilium (colonizes faster and gives larger flushes), but I consider tomentose mycelium to be healthy.  So, yes, different standards.

@Cegda I dump the dry vermiculite layer which exposes a mixture of mycelium and vermiculite, scrape off any excess, then I start digging into the mycelium from that region for the G2G transfer. I plop a scoop of the mycelium into the sterilized WBS and cap it. It's my understanding that a fully colonized substrate is fairly contamination resistant, so by keeping the colonized grain on top of the transfer jar it should help minimize contamination as long as the sterilization process was successful.

@LotKid Yeah, it might be missing some spokes, but it seems to roll lol

@JoBloShow I like steam sterilization. I use a rice cooker to get my WBS hydrated and it seems to work good enough and it avoids a 24 hour soak.

@ Brian Jones Yeah, I guess master jar might not be the correct term. What I mean by master jar is the originating jar that I colonized to use for the G2G transfer. Pftahahah, I own a pressure cooker, but I don't use it for anything. Anyway, there is nothing wrong with steam sterilization, experimenting, and enjoying the process. I'm simply sharing my experience.

Here is a progress picture of the 11 G2G transfer jars:



All of them seem to be colonizing beautifully.


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InvisiblePastywhyteMDiscord
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: checkmatsis]
    #26856305 - 07/31/20 09:38 PM (3 years, 5 months ago)

I have zero issue with tomentose mycelium, most people who have grown RW will attest that it’s pretty tomentose as a typical trait of the variety. Why would I have done that if I thought tomentose mycelium was unhealthy :shrug: pretty sure I can tell the difference between tomentose mycelium and a reaction to bacteria.


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Offlinesmalltalk_canceled
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Pastywhyte]
    #26856318 - 07/31/20 09:44 PM (3 years, 5 months ago)

and here i am boiling corn for 2 hours

did OP get shrooms yet


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Offlinecheckmatsis
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: smalltalk_canceled]
    #26856668 - 08/01/20 05:54 AM (3 years, 5 months ago)

@Pastywhyte Ah, yeah, I misunderstood your comment. I'm giving that jar some more time to colonize the top portion. If nothing changes by mid-week, then I'll do the smell test. Worst come to worst I'll remove the stalled section of the cake and use the healthy portion in the bulk grow.

@smalltalk_canceled I haven't gotten to fruiting yet. I'm sharing the process as I go along. So, I'm currently on the G2G transfer waiting for the 11 jars to fully colonize. Once that is finished, then I will be doing a shoebox inspired by ShaperDreaming SFF Shoebox Tek.


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InvisiblePastywhyteMDiscord
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: checkmatsis]
    #26856867 - 08/01/20 08:48 AM (3 years, 5 months ago)

Bacteria often doesn’t smell at all. I trust my eyes more than my nose.


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Offlinecheckmatsis
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Pastywhyte]
    #26856883 - 08/01/20 09:02 AM (3 years, 5 months ago)

@Pastywhyte All right, good to know. I'll keep that in mind. I'm still learning so I appreciate all the input.


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InvisibleTheDoobskerS
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: checkmatsis]
    #26857061 - 08/01/20 10:50 AM (3 years, 5 months ago)

Quote:

checkmatsis said:
I own a pressure cooker, but I don't use it for anything.




Wait, what? You own a PC, but you're choosing to steam sterilize grain jars instead?

What is your reasoning behind this? Are you trying to prove something?

That's not being stubborn. That's just choosing a sterilization process that is known to be less effective. You would never walk into a real bio lab and see steam sterilization being used. It will always be an autoclave. There's no need to debate this; it has been settled for years. But I'm just scratching my head trying to understand your reasoning here


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OfflineCegda
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: TheDoobsker]
    #26857623 - 08/01/20 06:03 PM (3 years, 5 months ago)

What he said ^

Especially since you're talking about making things faster... not only is the pc more likely to achieve sterilization, its going to run a lot faster than your 6 hour steam sterilization


Edited by Cegda (08/01/20 06:04 PM)


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InvisibleLotKid
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Cegda]
    #26857659 - 08/01/20 06:33 PM (3 years, 5 months ago)

Most new wheels claim to roll faster... :rockon:


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Offlinecheckmatsis
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Re: Grow Log: Master Jar to G2G to Shoebox. Let the journey begin! [Re: Cegda]
    #26857667 - 08/01/20 06:40 PM (3 years, 5 months ago)

@TheDoobsker It's simply how I'm choosing to do my grow and it has been working perfectly. Everything I have steam sterilized has colonized, so I'm completely happy with the results. There is nothing wrong with steam sterilization. If it didn't work, then it wouldn't be an option. Besides, how do you skin a cat? Anyway, I'm not debating anything. I'm simply sharing my current grow.

@Cegda As far as using a pressure cooker to save time, yeah, that is fully true. I might go rice cooker, pressure cooker, then refrigerator to see how fast I can prepare WBS for inoculation on my next grow.

@LotKid Pftahahaha very true.



I'll post a picture of the results of the G2G transfer once they have finished colonizing.


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