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OfflinePrietenul
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Would you risk using this LC? (PICs)
    #26776474 - 06/23/20 11:04 AM (3 years, 7 months ago)

I made a new Pan Texas LME-LC. It was very clear (I filtered it once) after PCing.

I put a wedge inside and after one day I could see tiny flakes, nothing strange, very clean...

Now, 6 days on the stirrer at ~75-78°F, it has grown considerably but there are some darker spots floating around. I can't say if those are pieces of the agar wedge or myc-clumps or contamination. Also, I have seen a longer thread that seems to be myc or dust/hair from before PCing, but I'm not sure.

What do you guys think? Can I use this?



This is the same LC, just under different background light.
Would you risk using this LC?
You may choose only one


Votes accepted from (06/23/20 02:03 AM) to (No end specified)
View the results of this poll



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OfflineGastronomicus
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Re: Would you risk using this LC? (PICs) [Re: Prietenul]
    #26776560 - 06/23/20 11:53 AM (3 years, 7 months ago)

No way to know from looks
Test it on agar


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Make my Funk the P Funk, I wants to get Funked up

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InvisibleZifozonke
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Re: Would you risk using this LC? (PICs) [Re: Gastronomicus]
    #26776571 - 06/23/20 11:56 AM (3 years, 7 months ago)

:whathesaid:
Rather be safe than sorry


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OfflinePrietenul
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Re: Would you risk using this LC? (PICs) [Re: Gastronomicus]
    #26776582 - 06/23/20 12:00 PM (3 years, 7 months ago)

Thanks for the reply.

Problem is, if I test it on agar, it means that I have to open the jar (contam vektor) and pull it into the syringe.

Taking only a little bit seems to be a high risk factor (pulling two times from same jar) so I would need to fill the syringe completely and let it sit around until the test-myc on agar has grown a bit (~2-3 days). I fear, that during that time the syringe can become contaminated.

I have only a SAB and I'm using syringes with very long needles that have no cap...

That's why I'm asking. To me atm it looks like a gamble, no matter what I do. So I might as well just use it and hope that it works. That's why I ask for opinions.


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Offlineichugwindex
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Re: Would you risk using this LC? (PICs) [Re: Prietenul]
    #26776647 - 06/23/20 12:38 PM (3 years, 7 months ago)

Test it on agar during the same session that you inoculate your jars? I know you are wanting to test it first but rolling the dice us part of the LC fun

Maybe fill a syringe and run your test agar and then just store the syringe until you see clean growth?


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Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.


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OfflinePrietenul
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Re: Would you risk using this LC? (PICs) [Re: ichugwindex]
    #26776957 - 06/23/20 02:26 PM (3 years, 7 months ago)

Quote:

ichugwindex said:
Test it on agar during the same session that you inoculate your jars? I know you are wanting to test it first but rolling the dice us part of the LC fun





That would be an option. I'm injecting the LC into (manure/straw)-bags. But obviously, if the LC turns out to be contaminated on agar, it'll be too late to save me from spending my precious manure and PCing those bags for 4 hours...

Quote:


Maybe fill a syringe and run your test agar and then just store the syringe until you see clean growth?




The storing part is my problem. I have no flow-hood, just an SAB, and I use long 14G needles, that have no caps to seal them.

Look:


Normally, I would just pull the LC from the jar, flame the needle, and inject into the bag immediately, then I seal the hole with a hot-glue pistol.
But obviously, I can do that only if I'm sure about the cleanliness of my LC.


Edited by Prietenul (06/23/20 02:29 PM)


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Offlineichugwindex
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Re: Would you risk using this LC? (PICs) [Re: Prietenul]
    #26777062 - 06/23/20 03:15 PM (3 years, 7 months ago)

Perhaps try LI instead of LC then if that's an option

https://www.shroomery.org/forums/showflat.php/Number/23739140/fpart/1/vc/1


--------------------
Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.


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Offlinepolaritymind
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Re: Would you risk using this LC? (PICs) [Re: ichugwindex]
    #26777135 - 06/23/20 03:51 PM (3 years, 7 months ago)

So a question why do you prefer LC over just agar? Because it seems to me that this precisely is the problem with LC. I used to think the point of LC is you add a lot, and there is a lot of inoculation points and the rye or whatever colonizes super fast. But now I realize, you cant add that much LC, because otherwise it fucks up the moisture content and the grain goes bacterial. Or doesnt it, how do you prevent this, what are you thoughts on this?


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Offlineichugwindex
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Re: Would you risk using this LC? (PICs) [Re: polaritymind]
    #26777173 - 06/23/20 04:05 PM (3 years, 7 months ago)

I use agar to make LC in these really tiny jars and they only get used a single time. As long as you are careful it's pretty cool. Some people don't like it because of its risky nature. Even if you are only using a drop or 2 it's faster than waiting for the myc to "leap" off the wedge. And if you are just doing brf jars you really can squirt a lot in there if you want


--------------------
Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.


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OfflinePrietenul
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Re: Would you risk using this LC? (PICs) [Re: polaritymind]
    #26777194 - 06/23/20 04:11 PM (3 years, 7 months ago)

Quote:

ichugwindex said:
Perhaps try LI instead of LC then if that's an option

https://www.shroomery.org/forums/showflat.php/Number/23739140/fpart/1/vc/1




I'm gonna look into it. Thanks.

Quote:

polaritymind said:
So a question why do you prefer LC over just agar? Because it seems to me that this precisely is the problem with LC. I used to think the point of LC is you add a lot, and there is a lot of inoculation points and the rye or whatever colonizes super fast. But now I realize, you cant add that much LC, because otherwise it fucks up the moisture content and the grain goes bacterial. Or doesnt it, how do you prevent this, what are you thoughts on this?




I'm trying to grow different Panaeolus species. There you have to either pasteurize straw/manure (which is a hassle) and then put your colonized grain in that, or you put the straw/manure together with grains in a bag, PC it and just inject some LC at the end. As I have no flow-hood, I find injecting to be the least risky inoculation. You flame your needle, inject into the sealed bag and immediately close the hole with hot-glue.

The moisture content is calculated to be ~63 percent when preparing the straw/manure/grain-bag, if I inject a certain amount of LC (240ml in my case) it should reach ~65 percent moisture content.

See Asura's writeup here:
https://www.shroomery.org/forums/showflat.php/Number/25987049

He explains it very well.


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OfflinePrietenul
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Re: Would you risk using this LC? (PICs) [Re: ichugwindex]
    #26777210 - 06/23/20 04:19 PM (3 years, 7 months ago)

Quote:

ichugwindex said:
Perhaps try LI instead of LC then if that's an option

https://www.shroomery.org/forums/showflat.php/Number/23739140/fpart/1/vc/1




Just to be sure. You're suggesting LI as opposed to LC because you think the risk of contams is lower, as the water has no nutrients in it. Right? Because the inoculating/extracting part is still the same, no matter if you use LI or LC. (and that is my main concern)


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OfflineMH5109
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Re: Would you risk using this LC? (PICs) [Re: Prietenul]
    #26777236 - 06/23/20 04:28 PM (3 years, 7 months ago)

Practice on little grain jars. Like these half pints, put 3/4 a cup of grain and test it on that.


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Offlineichugwindex
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Re: Would you risk using this LC? (PICs) [Re: Prietenul]
    #26777243 - 06/23/20 04:30 PM (3 years, 7 months ago)

Yes. I think the fact that you're going strait from the agar puck that you visually inspected might make you feel better emotionally as well lol i know it can be dreadful wondering if things are clean.

Btw I think your LC looks fine. I personally just stick with LC because I dont have fancy stir plates and stuff. Was just trying to give you options


--------------------
Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.


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OfflinePrietenul
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Re: Would you risk using this LC? (PICs) [Re: ichugwindex]
    #26777288 - 06/23/20 04:46 PM (3 years, 7 months ago)

Quote:

MH5109 said:
Practice on little grain jars. Like these half pints, put 3/4 a cup of grain and test it on that.





Sounds interesting. But on the other hand... if I can test on grain, I can test on agar as well :smile:

Quote:

ichugwindex said:
Yes. I think the fact that you're going strait from the agar puck that you visually inspected might make you feel better emotionally as well lol i know it can be dreadful wondering if things are clean.

Btw I think your LC looks fine. I personally just stick with LC because I dont have fancy stir plates and stuff. Was just trying to give you options





Yeah, there might be a little less risk involved.

You know what, I think I'll just inoculate and see what happens. Thanks for your input.


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OfflinePrietenul
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Re: Would you risk using this LC? (PICs) [Re: Prietenul]
    #26780583 - 06/24/20 10:21 PM (3 years, 7 months ago)

It seems that those spots in the LC are formed, when mycelium sticks to the glass walls, builds up clumps and gets torn off into the liquid by the stirrer again.


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