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OfflineWisdomSeeker
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Light yellow discoloration on mycellium in tub
    #26774531 - 06/22/20 03:20 PM (3 years, 7 months ago)

Im growing PE in my monotub and noticed slight discolorations of yellow on the mycellium:



Is this just metabolites or anything I should be concerned about? I have a UV-C light wand to kill bacteria and am thinking to run it over just in case. Wanted to hear your thoughts though.


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OfflineEntheoGod
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Re: Light yellow discoloration on mycellium in tub [Re: WisdomSeeker]
    #26774637 - 06/22/20 04:06 PM (3 years, 7 months ago)

Hmmm looks a bit off to me but I’m not aware of exactly what it is.

You can tell something is either bacterial or moldy. Every batch I have had with that many mutants, ended up contaminating at some point quickly after I noticed.

Also never heard of running a antibacterial light over it. I’d be afraid it would damage the mycelium if it were healthy. Notice all the mushies that look like they’re about to pop already or the mutated ones. The occasional mutant is fine or even sometimes desired depending on if the person isolated mutant genetics. But in assuming this was a MS Syringe and those mutants are caused by something bacterial or contaminated.


Edited by EntheoGod (06/22/20 04:09 PM)


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OfflineWisdomSeeker
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26774646 - 06/22/20 04:11 PM (3 years, 7 months ago)

Quote:

EntheoGod said:
Hmmm looks a bit off to me but I’m not aware of exactly what it is.

You can tell something is either bacterial or moldy. Every batch I have had with that many mutants, ended up contaminating at some point quickly after I noticed.

Also never heard of running a antibacterial light over it. I’d be afraid it would damage the mycelium if it were healthy. Notice all the mushies that look like they’re about to pop already or the mutated ones. The occasional mutant is fine or even sometimes desired depending on if the person isolated mutant genetics. But in assuming this was a MS Syringe and those mutants are caused by something bacterial or contaminated.





It is Penis Envy though so mutants are to be expected. Didnt use MS spore syringe. I just spent 10 minutes running it over with my UV-C wand. Ill keep doing it to see if it makes an impact. Really hoping its just metabolites.

You can find the wand here: https://www.midwestgrowkits.com/Portable-UV-C-Light-Sterilizer. They say its safe to use over the mushrooms.


Edited by WisdomSeeker (06/22/20 04:13 PM)


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OfflineEntheoGod
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Re: Light yellow discoloration on mycellium in tub [Re: WisdomSeeker]
    #26774659 - 06/22/20 04:15 PM (3 years, 7 months ago)

Okay 👌 what did you inoculate with or use as spawn?


Edited by EntheoGod (06/22/20 04:16 PM)


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OfflineWisdomSeeker
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26774694 - 06/22/20 04:36 PM (3 years, 7 months ago)

I used Midwest's premium 5 grain spawn bags and got the spore syringe from premiumspores.com. I let the bags colonize fully then inoculated the casing mix with it in my tub. 3 inches mixed with spawn then additional inch of casing layer. Field capacity was perfect when squeezed and I used the oven method when inoculating the spawn bags making sure to sterilize the needle with lighter.

Im still new to this so havent made my own substrate yet. Room temp is 72 degrees and I checked the humidity which has always been 95+. Once I stopped misting bc felt it was too much then the pins began to form. Most likely to the water evaporation. AC vent is in the closet so there is air circulation.


Edited by WisdomSeeker (06/22/20 04:38 PM)


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OfflineEntheoGod
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Re: Light yellow discoloration on mycellium in tub [Re: WisdomSeeker]
    #26774742 - 06/22/20 04:49 PM (3 years, 7 months ago)

So you did use an ms syringe or am I misreading? Ms syringes are never a guarantee.


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OfflineEntheoGod
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26774755 - 06/22/20 04:53 PM (3 years, 7 months ago)

Either way the tub doesn’t look very healthy. I hope a tc decides to chime in. As far as I can tell it’s definitely bacterial or contaminated in some way. That myc wouldn’t just make metabolites unless there was some form of stress being applied to them.
They make metabolites no matter what if left fully colonized for a while when they’re in the jars or spawn bag. Or if something is stressing them out.

Penicillin is literally made from metabolites of the penicillin mold being stressed or fighting off other molds. Not talking about penicillin but it’s same principle causing them both to make metabolites.

"Penicillin is a secondary metabolite of certain species of Penicillium and is produced when growth of the fungus is inhibited by STRESS. It is not produced during active growth"

Mushrooms dont just make metabolites when theyre healthy and thriving unless theyve been left alone for a long time.
So many folks make a post on here searching for a specific answer and when they dont get it they get angry. Dont be one of those folks. Learn from mistakes, improve, and move forward with the advice of the community here. If you cant look at your tub and see that those caps dont look right and the flush looks off then maybe take a look here. Ive been spreading this link like crazy and will continue to do so.

https://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1/vc/1


Edited by EntheoGod (06/22/20 05:07 PM)


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OfflineWisdomSeeker
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26774818 - 06/22/20 05:09 PM (3 years, 7 months ago)

Thanks Entheo. Yea I was just reading about the mycellium antibiotics from one of RR's old threads correcting someone not to call it Myc piss.

https://www.shroomery.org/forums/showflat.php/Number/6649453/fpart/2/vc/1#6649453

If you are interested, I will keep this thread updated. Continuing to try and kill off any bacteria and hope for the best. Not giving up just yet.

Oh and no, it wasnt MS. Just Penis Envy strain.


Edited by WisdomSeeker (06/22/20 05:11 PM)


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OfflineAtmozFear
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Re: Light yellow discoloration on mycellium in tub [Re: WisdomSeeker]
    #26774844 - 06/22/20 05:15 PM (3 years, 7 months ago)

Curious about that UV-C wand!  My air purifier in the other room has a UV-C light inside.


--------------------


Edited by AtmozFear (06/22/20 05:16 PM)


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OfflineEntheoGod
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Re: Light yellow discoloration on mycellium in tub [Re: WisdomSeeker]
    #26774852 - 06/22/20 05:18 PM (3 years, 7 months ago)

Good luck. FYI though, MS just means multispore syringe. Every spore syringe you will ever get will be a ms syringe. You cant have single genetics in a syringe full of spores. Every single spore even of the same strain, has different genetics. Thats what ms syringe means. You did use a ms syringe.

Dont give up. You got this.


EDIT: The only way you can have a syringe thats not multispore is if you were to isolate a single spores genetics and make inoculant out of that.


Edited by EntheoGod (06/22/20 05:19 PM)


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OfflineWisdomSeeker
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26774967 - 06/22/20 05:54 PM (3 years, 7 months ago)

Quote:

EntheoGod said:
Good luck. FYI though, MS just means multispore syringe. Every spore syringe you will ever get will be a ms syringe. You cant have single genetics in a syringe full of spores. Every single spore even of the same strain, has different genetics. Thats what ms syringe means. You did use a ms syringe.

Dont give up. You got this.


EDIT: The only way you can have a syringe thats not multispore is if you were to isolate a single spores genetics and make inoculant out of that.




Ah okay. I thought it meant cross strain like SSKS or something. This is where I bought it from:

https://premiumspores.com/spore-syringes/penis-envy-cubensis/

So curious, whats the reasoning behind the question of whether I used MS syringe? When would they not be or were you inquiring if I used my own spore prints or something?


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OfflineEntheoGod
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Re: Light yellow discoloration on mycellium in tub [Re: WisdomSeeker]
    #26774991 - 06/22/20 06:05 PM (3 years, 7 months ago)

I was asking about what you used because using a ms syringe and knowing you did EVERYTHING else right is a clear sign that the problem started with the syringe in the first place.

Ive decided to move to agar so I can get clean spawn every time before inoculating any grains or anything with it at all. I will always recommend everyone start with agar now that I have tried it and seen how easy it is to do. We only use the spores for microscopy by the way. But if youre getting syringes from any site, always use a sponsor on here to do the microscopy work. I learned that the hard way too. Bought from vendors that werent sponsors of the site and honestly have to say ive had much more success with sponsors of this site than any other place.

That being said, not every ms syringe will be contaminated but they're much more likely to have some form of contam or bacteria in them as opposed to using an agar wedge. You can drop a single drop of spore solution from a ms syringe onto agar once prepped properly and get single genetics, as well as being 100% sure that you arent putting any contams inside your substrate.

Clean spawn is most of the work when it comes to this stuff. Everything else can basically be done in open air. I shouldnt say everything as to mislead anyone. What I mean is when you have clean spawn it wont really matter much about whether the substrate is prepped in sterile tech. With CVG you can wipe your ass and go right back to mixing the tub as long as your spawn is clean and youve prepped everything correctly.
Try getting into agar and learning that. It will teach you if your issue lies in your sterile technique or if it was purely the spores you received. I had 4 syringes from a non sponsor of the site, and every single thing I did with those ended up super weak and eventually contaminated.

BTW I still have yet to even use agar to inoculate anything. Im working on learning everything I can about it to do it properly. Ive successfully inoculated some agar though and am currently watching for a good chunk to transfer and attempt cleaning up. I even used a known bacterial syringe to do the inoculating. You can get clean spawn from a bunk syringe if you use agar. 


Edited by EntheoGod (06/23/20 07:51 AM)


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OfflineWisdomSeeker
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26775060 - 06/22/20 06:31 PM (3 years, 7 months ago)

Hmmm this sounds too good to be true. Im intrigued. Can you send me any links to any agar teks you've been reading up on? Would like to learn more.


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OfflineEntheoGod
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Re: Light yellow discoloration on mycellium in tub [Re: WisdomSeeker]
    #26775120 - 06/22/20 06:48 PM (3 years, 7 months ago)

These are the links I am currently using. All the info you need can be found in both articles but be sure to use the search function for anything at all you may be confused about. :smile:

Glad I can help you out! I thought it was too good to be true as well.

https://www.shroomery.org/forums/showflat.php/Number/23188000
https://www.shroomery.org/forums/showflat.php/Number/19208976
https://www.shroomery.org/forums/showflat.php/Number/20846749
https://www.shroomery.org/forums/showflat.php/Number/24330957#24330957
https://www.shroomery.org/forums/showflat.php/Number/18430998


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OfflineAtmozFear
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26775235 - 06/22/20 07:31 PM (3 years, 7 months ago)

Quote:

EntheoGod said:
With CVG you can wipe your ass and go right back to mixing the tub as long as your spawn is clean and youve prepped everything correctly.




:lmafo:


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OfflineJustweed
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Re: Light yellow discoloration on mycellium in tub [Re: AtmozFear]
    #26775290 - 06/22/20 07:57 PM (3 years, 7 months ago)

The tub looks totally fine...Not sure what EG is seeing that makes him think it's off.

If your sub puts out a small bit of yellow liquid, that's just 'piss' from the sub, it happens. This late in the game, an infection will be obvious, it'll be a bright color. It is possible that if your sub starts putting out a lot of 'piss' that there's an infection inside the sub, so watch for that.


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Keep 'em high and tight guys....


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OfflineEntheoGod
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Re: Light yellow discoloration on mycellium in tub [Re: Justweed]
    #26775430 - 06/22/20 08:57 PM (3 years, 7 months ago)

Quote:

Justweed said:
The tub looks totally fine...Not sure what EG is seeing that makes him think it's off.

If your sub puts out a small bit of yellow liquid, that's just 'piss' from the sub, it happens. This late in the game, an infection will be obvious, it'll be a bright color. It is possible that if your sub starts putting out a lot of 'piss' that there's an infection inside the sub, so watch for that.




Myc piss isn’t a thing btw I’m not finding the post this is mentioned in by roger rabbit himself talks about this. Trust what you wish but I promise you that tub will go bad in a few days time. I’ve given you all the info you need to deduce this on your own. Metabolites or “piss” is a direct product of stress on the mycelium. Stress can be anything from temp fluctuations to a bacteria thriving inside the substrate. Google mushroom metabolites or just search on here and you’ll see OP.
I really hope a TC can show you the correct info so it’s more believable. Use the advanced search and look up why mushrooms produce metabolites. You’ll get your answer really quickly.


Edited by EntheoGod (06/22/20 09:01 PM)


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OfflineJustweed
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26775447 - 06/22/20 09:06 PM (3 years, 7 months ago)

Quote:

EntheoGod said:
Myc piss isn’t a thing btw I’m not finding the post this is mentioned in by roger rabbit himself talks about this. Trust what you wish but I promise you that tub will go bad in a few days time. I’ve given you all the info you need to deduce this on your own. Metabolites or “piss” is a direct product of stress on the mycelium. Stress can be anything from temp fluctuations to a bacteria thriving inside the substrate. Google mushroom metabolites or just search on here and you’ll see OP.
I really hope a TC can show you the correct info so it’s more believable. Use the advanced search and look up why mushrooms produce metabolites. You’ll get your answer really quickly.





You say myc piss isn't a thing, then you describe what causes myc piss...Yes, it's metabolites, caused by various things. I don't see any on this sub though in the picture. You're right though we can just wait a few days and see!


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Keep 'em high and tight guys....


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Re: Light yellow discoloration on mycellium in tub [Re: Justweed]
    #26775461 - 06/22/20 09:15 PM (3 years, 7 months ago)

Saying myc piss will give the impression it’s normal, when it is not.

You can likely let that flush play out but I almost guarantee you won’t get another out of it before you see a contamination. Healthy mycelium is bright white and beautiful tbh. I hope the op decides to look this all up instead of trusting you or I. It’s best to learn for oneself.


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OfflineWisdomSeeker
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Re: Light yellow discoloration on mycellium in tub [Re: EntheoGod]
    #26776014 - 06/23/20 07:03 AM (3 years, 7 months ago)

Quote:

EntheoGod said:
Quote:

Justweed said:

Quote:


Myc piss isn’t a thing btw I’m not finding the post this is mentioned in by roger rabbit himself talks about this. Trust what you wish but I promise you that tub will go bad in a few days time. I’ve given you all the info you need to deduce this on your own. Metabolites or “piss” is a direct product of stress on the mycelium. Stress can be anything from temp fluctuations to a bacteria thriving inside the substrate. Google mushroom metabolites or just search on here and you’ll see OP.
I really hope a TC can show you the correct info so it’s more believable. Use the advanced search and look up why mushrooms produce metabolites. You’ll get your answer really quickly.





I'm referring to the link I pasted above where RR says:

"It isn't piss and it isn't waste material. It's antibiotics. There is no such thing as mycelium piss. This piss bull gets repeated by noobs enough that other noobs think it's true and then they repeat it. Disinformation dies hard, and I've been trying to kill this one for years. Stamets has been trying to kill it for twenty years without luck because people without knowledge keep repeating it over and over.
RR"

People just call it that to easily recognize what we are talking about.

I have looked up the info needed. There is no conclusive evidence thats its bacteria. Stress can be caused by many things. I did drop the temp in there 5 degrees and lossed polyfill. Many factors could cause this and Im not dismissing bacteria either.

I said I would monitor and keep this thread updated so we all have a definitive answer. I have hit the surface with my UV-C light for 15 minute sessions yesterday and another 5 minute session this morning. I will continue 3 5 minute sessions per day. If someone wants to debate it will kill the mycellium then I did a fair amount of research on this topic as well. It wont if you restrict the time. In fact, in many cases made it grow faster.

Anywho, here is the latest pic. Still seems like mushrooms are growing fine. See on the caps those circles indented...? Thats what happens when 3water droplets collect on tub lid and drop down hitting the cap. No bueno.




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