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Invisiblewizard2k
life-scientist

Registered: 05/26/03
Posts: 74
Loc: NRW
Isolate a single spore?
    #2677562 - 05/14/04 01:10 PM (14 years, 16 days ago)

Hi,
if I want to grow monocaryotic mycelium, how can I isolate a single spore? Is there a method at all?
Thank you!


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Looking for: Volvariella volvacea, Panaeolus Cyanescens and rare edibles. Trades are welcome!


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Invisibletoad857
President of theUnited States

Registered: 02/02/04
Posts: 283
Re: Isolate a single spore? [Re: wizard2k]
    #2677592 - 05/14/04 01:18 PM (14 years, 16 days ago)

you could do some tricky slide/plate work under a microscope. it's possible for sure. it just may be tedious.


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OfflineGr0wer
always improving
Male

Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX Flag
Last seen: 2 months, 16 days
Trusted Cultivator
Re: Isolate a single spore? [Re: wizard2k]
    #2677612 - 05/14/04 01:24 PM (14 years, 16 days ago)

agar isolation.


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Invisiblewizard2k
life-scientist

Registered: 05/26/03
Posts: 74
Loc: NRW
Re: Isolate a single spore? [Re: toad857]
    #2677616 - 05/14/04 01:25 PM (14 years, 16 days ago)

You would need a strong microscope, then?
I just had the idea to make a highly diluted spore solution and use less than a drop of it. But you can't be sure to have monocaryotic mycelium, this way (it might be even unlikely).


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Looking for: Volvariella volvacea, Panaeolus Cyanescens and rare edibles. Trades are welcome!


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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