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OfflinePackerbacker12
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Is this cobweb or some other contaminate?
    #26774803 - 06/22/20 05:04 PM (3 years, 7 months ago)

Hello shroomery family,

My past few attempts at shoeboxes have ended in contamination. I am trying to track the culprit and im thinking its my rye berries? I have always done the RR rye tek but my jars have not been looking perfect in my opinion. Multiple have spots that either look bacterial? (Yellow spotting) and or infected with cobweb mold. Can anyone confirm if these jars look okay? These are how my last ones looked and the tubs never made it so i believe its a contamination. They smell fine though.i just switched to whole oats and im hoping to have better results. All of these jars were inoculated via clean agar wedge so i don't think thats my problem.



Heres a jar that i think looks clean for reference.

https://files.shroomery.org/files/20-26/286702325-20200622_083121.jpg

Any help would be great! Thank you!


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Invisiblenix21
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Re: Is this cobweb or some other contaminate? [Re: Packerbacker12]
    #26774840 - 06/22/20 05:14 PM (3 years, 7 months ago)

All of them are heavy bacterial, even "clean" one, and on some jars mold is obvious (bottom two)... 1)your agar isnt clean 2)your inoculation procedure is off 3) grain prep... pics of your agar plates and grain jars before inoculation will help us to give you answer on 1) and 3)


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OfflinePackerbacker12
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Re: Is this cobweb or some other contaminate? [Re: nix21]
    #26774938 - 06/22/20 05:47 PM (3 years, 7 months ago)

Thanks for the reply! Would you scrap all of them? Im sick of tubs contaminating so i dont mind just starting over.

My agar appears to be clean. What is the best way to take a picture?

I work in a SAB so should i just take the lid off and snap a pic of the plate inside the sab? Ive had issues with my rye before and thats why i switched to oats recently. I always pc @15psi for 90-120 minutes.


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OfflineJustweed
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Re: Is this cobweb or some other contaminate? [Re: Packerbacker12]
    #26775317 - 06/22/20 08:08 PM (3 years, 7 months ago)

Yes those jars need thrown out.

If you have cobweb or bacteria on your agar it should be apparent. Do a transfer and see if it grows in a regular pattern or if it has obvious contaminants.

PC your jars for 95 minutes @15 PSI. You might want to revisit your grain prep, usually bacteria go nuts for overly wet grain more than they will dryer grain, even with proper PCing.


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Keep 'em high and tight guys....


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OfflinePackerbacker12
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Re: Is this cobweb or some other contaminate? [Re: Justweed]
    #26775452 - 06/22/20 09:11 PM (3 years, 7 months ago)



Okay what about these two jars? To me these look much better but maybe im missing something.

That's how my agar plates look before i ever inoculate. They seem good to me. Must be my grain prep?


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OfflineJustweed
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Re: Is this cobweb or some other contaminate? [Re: Packerbacker12]
    #26777327 - 06/23/20 05:05 PM (3 years, 7 months ago)

Those jars look better. What's the dark line on the outside from?
Those plates look alright to me, but I'm not an agar expert.


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Keep 'em high and tight guys....


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OfflinePackerbacker12
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Re: Is this cobweb or some other contaminate? [Re: Justweed]
    #26777576 - 06/23/20 07:04 PM (3 years, 7 months ago)

The dark line? On the first pic? Its just a rust line on the outside of the jar. This two are almost 3 weeks old though so im worried they're stalled out. Doing all new transfers tomorrow


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OfflineJustweed
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Re: Is this cobweb or some other contaminate? [Re: Packerbacker12]
    #26780104 - 06/24/20 06:52 PM (3 years, 7 months ago)

What's the general temperature where your jars are?


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Keep 'em high and tight guys....


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OfflinePackerbacker12
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Re: Is this cobweb or some other contaminate? [Re: Justweed]
    #26780522 - 06/24/20 10:01 PM (3 years, 7 months ago)

Lately its been in the low 70s around 71-72 but usually around 66-70 most of the year.


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