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Invisiblenatedawgnow
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How long will an unsealed spawn bag last? Let's find out! * 4
    #26664387 - 05/11/20 12:11 PM (3 years, 9 months ago)

How long will an unsealed spawn bag last on the shelf? The short answer is a long fuckin time!

Once every 6 months or so I do a control run where I make a jar, bag, or stack of
5 petris and I leave them be to make sure filters and hood are working as they should (rhyme master:grin:).

My last control run was in february and after a month everything was fine, petris and jars
got used, but I wanted to see how long a non impulse sealed bag would last sitting
on my workstation.

Here are a couple pics of the bag in its natural habitat


The bag has 0 signs of visible contamination. The opening is held shut not by an
impulse seal as indicated in the pic below, but instead it is held closed by a vacuum
created upon cool down of the pc.
(note there is no impulse seal line)


So, unsealed bags can last quite a while on the shelf, but will it grow a tub?
Let's find out! I will be inoculating this bag in a couple hours and will be
updating this thread with its progress!

Quote:

natedawgnow said:
Update 5/23:

This bag has been colonized for a few days now, just forgot to update.


It looks identical to my other bags, no signs of contam. Gonna spawn it in a few
days, as soon as my current cycle is done!



Quote:

natedawgnow said:
Well here's an update!

Slacked hard and didnt get around to spawning til last night. Some pics


Ill update as it progresses but so far so good!



Quote:

natedawgnow said:
Alright here we are at day 11! Tub looks great, same as the other 20


Pinning like mad





Edited by natedawgnow (06/14/20 09:36 AM)


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Offlinetripdawg420
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26664390 - 05/11/20 12:12 PM (3 years, 9 months ago)

:thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: tripdawg420] * 1
    #26665582 - 05/11/20 10:45 PM (3 years, 9 months ago)

Bag has been inoculated with half a qt of toc spawn. I'll update as it progresses!


--------------------


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InvisibleModularMind
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26665661 - 05/12/20 12:25 AM (3 years, 9 months ago)

:popcorn:


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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: ModularMind] * 1
    #26690652 - 05/23/20 03:20 PM (3 years, 8 months ago)

Update:

This bag has been colonized for a few days now, just forgot to update.


It looks identical to my other bags, no signs of contam. Gonna spawn it in a few
days, as soon as my current cycle is done!


--------------------


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Invisiblefilthyknees
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26691020 - 05/23/20 07:09 PM (3 years, 8 months ago)

So you let it sit three months?


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: filthyknees]
    #26691036 - 05/23/20 07:14 PM (3 years, 8 months ago)

Yup. Just sat exactly where it is in the pics for 3 months.


--------------------


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OfflineLogicaL ChaosM
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26691037 - 05/23/20 07:14 PM (3 years, 8 months ago)

So the "Control" bag has mycelium in it?

Hella confused but interesting experiment :strokebeard:


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: LogicaL Chaos]
    #26691046 - 05/23/20 07:19 PM (3 years, 8 months ago)

Haha no. Every 6 months I make a bag, jar, and some plates and just leave
them to make sure my hood and filters are working. They get labelled "control"

The petris get opened in front of the hood for a few minutes and resealed, jars
and bags usually just sit on my desk but sometimes I'll open the jar in front of
the hood, flame my scalpel, and drop a blank agar wedge in and see what happens.

I decided to let the spawn bag sit for a few months to see if an unsealed spawn bag
will contam on its own. It didn't, at least not that I could tell, so I decided to see how well it will grow
a tub so I knocked it up with a half jar of spawn and now I'm gonna spawn it
in a couple days.  If the tub doesn't contam then I know it didn't get compromised
during the 3 months it sat on a shelf.


--------------------


Edited by natedawgnow (05/23/20 10:58 PM)


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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26718589 - 06/04/20 08:09 AM (3 years, 8 months ago)

Well here's an update!

Slacked hard and didnt get around to spawning til last night. Some pics


Ill update as it progresses but so far so good!


--------------------


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Offlinelefty24
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26718600 - 06/04/20 08:15 AM (3 years, 8 months ago)

I am not surprised. I've had great results using tinfoil for lids on pftek.


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OfflinePutrema
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Registered: 02/20/20
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: lefty24]
    #26718631 - 06/04/20 08:38 AM (3 years, 8 months ago)

Hello, Im registered here for a while but this is my first post. In my last pressure cooking session, there I filled a bag with rye. This bag I only wrapped by its own, but I cant see any contamns. But I think the contams are waiting where the bag is slightly open. How can I handle it correctly? Is there the possibility that I can wash a glass with hot water and fill it with the bag and throw a piece from agar in it in my SAB? Im too lazy, I have some spontanous fructifications in my dishes, but today I did nothing - again. Well, but my first rye glass with casing is producing flesh right now, hopefully I will learn how to run with mycelium.


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OfflineA.k.aM
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: Putrema]
    #26719356 - 06/04/20 02:28 PM (3 years, 8 months ago)

Did you rehydrate the grains somehow before inoculating?


--------------------
LAGM2020


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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: A.k.a]
    #26719371 - 06/04/20 02:35 PM (3 years, 8 months ago)

No. That's pretty much the point of the whole experiment :lol:

That and the contam part


--------------------


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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26743717 - 06/14/20 09:34 AM (3 years, 7 months ago)

Alright here we are at day 11! Tub looks great, same as the other 20


Pinning like mad


--------------------


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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26768063 - 06/21/20 07:55 PM (3 years, 7 months ago)

Last update for this thread probably unless i let this tub go for a 2nd flush.

Here she is today:


So, in conclusion, if you properly sterilize and your spawn is clean, you can
let bags sit shelved for quite a while!

The bag that made this tub, to refresh everyones memory, was made in February, knocked up in may and fruited in june.
That's 4 months! Hope you all enjoyed following along!


--------------------


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InvisiblePastywhyteMDiscord
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: natedawgnow]
    #26768092 - 06/21/20 08:03 PM (3 years, 7 months ago)

Quote:

natedawgnow said:

So, in conclusion, IF you properly sterilize and your spawn is clean, you can
let bags sit shelved for quite a while!





That’s a big “if” however where your concerned I’m not surprised by the result. Nice grow man :rockon:


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Invisiblenatedawgnow
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Re: How long will an unsealed spawn bag last? Let's find out! [Re: Pastywhyte]
    #26768124 - 06/21/20 08:21 PM (3 years, 7 months ago)

Thanks man! Glad to see you around and posting!

Definitely probably not the best idea to let a bag sit for months unsealed
if you arent confident in your grow skills, but definitely no reason to trip if
you can't get to knockin up your bags the day after you make them.

On average my bags sit for a few days no matter what cause i make all my bags then
knock them up all at once. Usually takes me 4 cycles to cook all the bags I need
and I spread that out over a few days.


--------------------


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