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Invisiblemaxmush
Always learning...
Registered: 06/13/20
Posts: 440
Print to agar - sterile tek (?)
    #26762984 - 06/21/20 12:24 PM (3 years, 7 months ago)

Once again i am getting ahead of myself (lol). I am still fairly new hands on, but have rabbit holed on many topics and teks now for a while now.

Anyway, my question is this: we spend so much time and effort using sterile tek to inoculate agar from prints (or MS syringe) which i completely understand, but arent the prints already likely contaminated when then you introduce this into the sterile environment? Is one of the purposes of transfer to agar to grow myc and the possible contams so you can then isolate the "healthy" myc to another agar dish to separate?

Thanks in advance!


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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OfflineTrichodingus
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Registered: 05/31/20
Posts: 85
Loc: Communing with the divine
Last seen: 3 years, 6 months
Re: Print to agar - sterile tek (?) [Re: maxmush] * 1
    #26763014 - 06/21/20 12:48 PM (3 years, 7 months ago)

Bingo!
Agar is to isolate and propagate clean cultures.

If it's contaminated, transfer a clean section to a new plate.

Repeat until it's just healthy myc!


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OfflineBrian Jones
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Registered: 12/18/12
Posts: 12,340
Loc: attending Snake Church
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Re: Print to agar - sterile tek (?) [Re: Trichodingus]
    #26763203 - 06/21/20 02:12 PM (3 years, 7 months ago)

That's it.


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"The Rolling Stones will break up over Brian Jones' dead body"    John Lennon

I don't want no commies in my car. No Christians either.

The worst thing about corruption is that it works so well,


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Invisiblemaxmush
Always learning...
Registered: 06/13/20
Posts: 440
Re: Print to agar - sterile tek (?) [Re: Brian Jones]
    #26766278 - 06/21/20 06:51 PM (3 years, 7 months ago)

Got it! 

So what are the other possible advantages of transferring to agar from an inoculation standpoint (yes I have read previous threads :wink:).

The benefit of cleaning out contams is (now) an obvious advantage, what else?

Does it propagate faster and have a better success rate than an MC syringe (and if so, is this because of the "cleaner myc"?).

Are prints "better" than syringes and/or tissue cultures?

Sorry if these points have been hammered on previously, but I would love to have further clarity on it if thats cool :smile:


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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