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OfflineBiscuits
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Agar straight to grain from fridge?
    #26756299 - 06/19/20 04:14 AM (3 years, 7 months ago)

Hi,

If I have preserved a strain in a fridge for a month or two, do I need to transfer it to some fresh agar or anything or can I take it straight to grain?


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OfflineMycobro420
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Re: Agar straight to grain from fridge? [Re: Biscuits]
    #26756427 - 06/19/20 06:36 AM (3 years, 7 months ago)

I stored Petri dishes in fridge inside insolated lunch box.if you had them in your normal fridge and not a personal myco fridge.they could be damaged from tempature swings from you opening an closing the fridge to get food. A myco fridge is not used as much as normal fridge.so I say if not in lunch box is re transfer


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OfflineBiscuits
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Re: Agar straight to grain from fridge? [Re: Mycobro420]
    #26756444 - 06/19/20 06:51 AM (3 years, 7 months ago)

Cool thank you I'll do that :smile:


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OfflineMycobro420
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Re: Agar straight to grain from fridge? [Re: Biscuits]
    #26756687 - 06/19/20 08:41 AM (3 years, 7 months ago)

Next time for storage get insolated lunch box.can last for years like that with no culture slants.the purpose of it is the culture wount degrade from temp swings


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Offlinemushpunx
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Re: Agar straight to grain from fridge? [Re: Biscuits]
    #26756770 - 06/19/20 09:13 AM (3 years, 7 months ago)

As long as you don't have reason to believe it's contaminated it's fine. For plates I'm putting into cold storage I only let them colonize 3/4ths of the way and take them out a few days before I use them to make sure they are clean.

I also like to keep master plates stored in the fridge and when I want to use it I'll take a grain of rice size transfer to a blank plate and grow it out for a few days.

Lunch box is a good idea to have to store cultures in. I used to use one


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OfflineBiscuits
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Re: Agar straight to grain from fridge? [Re: mushpunx]
    #26759071 - 06/20/20 03:23 AM (3 years, 7 months ago)

An insulated lunch box seems like a good idea, but what I have is exactly what you said -- a 3/4 colonized plate, and it seems clean (it has been subject to temperature swings though)

You know what, I'm going to go ahead and take it straight out of the fridge to 6 quarts of grain and see if it's slower than usual or contaminated or whatever, to test the hypothesis. For science or something!


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OfflineMycobro420
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Re: Agar straight to grain from fridge? [Re: Biscuits]
    #26759722 - 06/20/20 11:59 AM (3 years, 7 months ago)

The reason I do this is cause before I had almost fully colinized plates.took them out to do transfer an make a bunch of new dishes.all agar was clean before I put in fridge (without lunchbox)then after I did transfer probly lil under half had bacteria in it witch is unusual for me especially since I have a flowhood. But I just retransfer then again all came back fine.just my experience but I credit the bacteria to the temp swing could be wrong but my experience


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Offlinemushpunx
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Re: Agar straight to grain from fridge? [Re: Mycobro420]
    #26760456 - 06/20/20 06:59 PM (3 years, 7 months ago)

Quote:

Mycobro420 said:
The reason I do this is cause before I had almost fully colinized plates.took them out to do transfer an make a bunch of new dishes.all agar was clean before I put in fridge (without lunchbox)then after I did transfer probly lil under half had bacteria in it witch is unusual for me especially since I have a flowhood. But I just retransfer then again all came back fine.just my experience but I credit the bacteria to the temp swing could be wrong but my experience





If you are using pitres what are you wrapping them with?

I wonder if the temp swing would be enough to cause a clean plate to contaminate. For that to happen there would have to be contams already in the plate or they are finding their way in to the plate somehow


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OfflineMycobro420
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Re: Agar straight to grain from fridge? [Re: mushpunx]
    #26762151 - 06/21/20 03:04 AM (3 years, 7 months ago)

I use parafilm I was surprised to. bacteria is visible noticable on plates saw non before transfers after I took myc from edge of dish an put to new plates alot had bacteria then.not sure why but it is unusual for that to happen.after I started using lunch box haven't had that issue


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Offlinemushpunx
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Re: str aight to grain from fridge? [Re: mushpunx]
    #26762335 - 06/21/20 06:05 AM (3 years, 7 months ago)

Quote:

Mycobro420 said:
I use parafilm I was surprised to. bacteria is visible noticable on plates saw non before transfers after I took myc from edge of dish an put to new plates alot had bacteria then.not sure why but it is unusual for that to happen.after I started using lunch box haven't had that issue




It happens I guess :shrug:
The lunch box type things are a good idea for the fridge. So is making sure the temperature is dialed in right.

I had a couple mini fridges for cultures before.
They were devoted to storing slants and plates so temperature swings from opening the door a lot.

They worked but the temps inside varied depending on where they were. Some at the top or bottom froze. Others the agar turned to into mush.

Lost a lot of good cutures that day


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Edited by mushpunx (06/21/20 07:42 AM)


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OfflineMycobro420
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Re: str aight to grain from fridge? [Re: mushpunx]
    #26762376 - 06/21/20 06:55 AM (3 years, 7 months ago)

Ya the temps for storing agar I belive are 35-37f I'd have to double check tho. Never had a plate freeze or mush.the lunchbox is good cause if it's insulated there is no temp swings inside the lunch box so u can store it in your normal fridge.i read a Tek from a Tc that said if temps are right you can store plates for 10 years without culture slants.onky down side is there more bulky than slants.


Edited by Mycobro420 (06/21/20 06:55 AM)


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OfflineBiscuits
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Re: str aight to grain from fridge? [Re: Mycobro420]
    #26796267 - 07/01/20 05:29 AM (3 years, 7 months ago)

So just a little update...

Took the plate out from the fridge on 21/06, let it sit for a full day. Minor blue shade from it, probably from bruising while adjusting to the temperature. No contaminants on the agar were visible. Transferred the plate to 6 quarts of rye.

10 days later and growth seems to be on par. Usually takes me 20 days from the agar drop to spawning, so we will see how it goes. Doesn't seem to be any obvious contaminants and probably faster than doing an agar transfer between, but we will see how it develops.



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Invisiblesandman420
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Re: Agar straight to grain from fridge? [Re: Mycobro420]
    #26796284 - 07/01/20 05:48 AM (3 years, 7 months ago)

Quote:

Mycobro420 said:
Next time for storage get insolated lunch box.can last for years like that with no culture slants.the purpose of it is the culture wount degrade from temp swings




the culture doesnt just degrade from temp swings per se, it gets swamped with condensation water. I've found a plate nearly half full of water before from bad storage.

It's not a bad idea to put a parafilm wrapped plate inside of a ziplock before putting it in your lunch box. Parafilm had a bad tendency to have rips and tears suddenly appear out of nowhere it seems no matter how gently you try to stretch it. Probably mostly old stock being sold to the public while real labs get the fresh rolls. Doesn't really happen on tubes and slants because they doesnt have that sharp edge that petri dishes have. So wrap it up in a baggie.


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OfflineBiscuits
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Re: Agar straight to grain from fridge? [Re: sandman420]
    #26824529 - 07/15/20 07:23 AM (3 years, 6 months ago)

Ok went to spawn them all today, they all looked slightly bacterial but on opening them they had a strong odour of formic acid -- definitely bacterial.

0/10 would not recommend fridge agar to grain.


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Offlinemushpunx
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Re: Agar straight to grain from fridge? [Re: sandman420]
    #26825103 - 07/15/20 12:24 PM (3 years, 6 months ago)

Quote:

sandman420 said:
Quote:

Mycobro420 said:
Next time for storage get insolated lunch box.can last for years like that with no culture slants.the purpose of it is the culture wount degrade from temp swings




the culture doesnt just degrade from temp swings per se, it gets swamped with condensation water. I've found a plate nearly half full of water before from bad storage.

It's not a bad idea to put a parafilm wrapped plate inside of a ziplock before putting it in your lunch box. Parafilm had a bad tendency to have rips and tears suddenly appear out of nowhere it seems no matter how gently you try to stretch it. Probably mostly old stock being sold to the public while real labs get the fresh rolls. Doesn't really happen on tubes and slants because they doesnt have that sharp edge that petri dishes have. So wrap it up in a baggie.




I was having this problem recently- kept getting mold at the edge of inoculated plates (my blank plates were wrapped once and placed back in their sleeve). Took me a minute to catch the tiny tears at the plate's edge. It's hot here right now but this roll is a few years old so I think that's the issue.
My sister works in a lab and has a personal account, next time she comes up I'm getting the hook up with the fresh stuff :lol:


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InvisibleBsdgaou

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Re: Agar straight to grain from fridge? [Re: mushpunx]
    #26826164 - 07/16/20 12:19 AM (3 years, 6 months ago)

Maybe it's better to store in the fridge on - 18°C?


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OfflineMycobro420
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Re: Agar straight to grain from fridge? [Re: Bsdgaou]
    #26826581 - 07/16/20 09:16 AM (3 years, 6 months ago)

Recently I had really rizomorphic growth on plates my storage was same. I wraped dishes with parafilm put each dish in ziplock bag. Inside insulated lunch box inside fridge stored for about a month an half.wen I transfer to new plates I had half rizo growth half tomentose growth I was surprised.i head myc can get lazy if you keep transfering to the same nutritious medium like using mea for many transfers.i put most to grain and are doing fine.i try to not lay dishes dormant and use them at fully colinized for best results


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