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Moopers
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Growing P. papuana
#26752707 - 06/17/20 08:15 PM (3 years, 7 months ago) |
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I purchased a print of Psilocybe papuana, and I'm hoping to grow it out. I understand I'm in mostly uncharted territory here, with only a few folks on shroomery growing this species. I think it would be great to consolidate knowledge, now that a vendor currently has prints for sale. Anyone else growing this?
I would love any guidance or knowledge you all can share. I've sent a PM to inski to inquire about his methods, and I'll be very thankful if we wants to/has time to write them up. I thought I'd put it out there for anyone else who is growing or has grown P. papuana. Any advice on what works and doesn't?
It's been described as "clay-loving", rather than as coprophilous or lignicolous. It's been recently found in NSW Australia by Heyowana, growing in clay soil near where logs were sawn year after year (Eucalyptus spp.). Given this, is it more of a lignicolous species? I believe it also fruits best in fall?
I plan to put spore print to agar, agar to grain (brown rice or rye). I have some ideas to experiment from there, but want to know what's been done/what worked/what failed/etc.
Edited by Moopers (06/20/20 07:28 PM)
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Moopers
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Re: Advice on growing P. papuana [Re: Moopers]
#26757107 - 06/19/20 11:21 AM (3 years, 7 months ago) |
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Put spores to MYA yesterday, 4 plates. I'll post pics if/when they show germination.
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exzile
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Re: Advice on growing P. papuana [Re: Moopers]
#26757199 - 06/19/20 11:55 AM (3 years, 7 months ago) |
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Following.
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Celestialexplorer1



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Re: Advice on growing P. papuana [Re: Moopers]
#26757203 - 06/19/20 11:56 AM (3 years, 7 months ago) |
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Don’t know much about them. There’s really next to no information on them on the web. I’m definitely interested in knowing more though.
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mushpunx
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These are seriously cool looking! My first time seeing these.
Following!
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Moopers
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Re: Advice on growing P. papuana [Re: mushpunx]
#26759686 - 06/20/20 11:33 AM (3 years, 7 months ago) |
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Germination on 2 plates after 2 days, not bad. Plates were kept 71-74F. The haze is condensation.
Edited by Moopers (06/20/20 03:07 PM)
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Moopers
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Re: Advice on growing P. papuana [Re: Moopers]
#26793625 - 06/30/20 02:28 AM (3 years, 6 months ago) |
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What started so clean looking is now quite a mess. Truly a spore print done in the wild. It's going to take a few transfers before these first cultures are useable.
The P. papuana myc is thick and bright, like cream. Three plates had usable cultures. The fourth plate was all contam along the inoculation zigzag.
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Nobler Hino
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Re: Advice on growing P. papuana [Re: Moopers]
#26794310 - 06/30/20 11:08 AM (3 years, 6 months ago) |
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That flat growth reminds me of tamp mycelium. I would say you have some nice areas to transfer from.
Just noticed where you cut, that thick shit is what I would avoid.
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Edited by Nobler Hino (06/30/20 11:09 AM)
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Moopers
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Re: Advice on growing P. papuana [Re: Nobler Hino]
#26794326 - 06/30/20 11:21 AM (3 years, 6 months ago) |
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Quote:
Nobler Hino said: That flat growth reminds me of tamp mycelium. I would say you have some nice areas to transfer from.
Just noticed where you cut, that thick shit is what I would avoid.
What's wrong with the thick stuff? Those are the spots that first germinated, and I was avoiding the flat faint/powdery white areas because there's so much discoloration I assume is contam. That was my reasoning, but I'm happy to be educated if I chose poorly.
Where would you suggest? I can always take more transfers.
Edited by Moopers (06/30/20 11:25 AM)
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inski
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Re: Advice on growing P. papuana [Re: Moopers]
#26794461 - 06/30/20 12:37 PM (3 years, 6 months ago) |
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Make lots of transfers from the streak at the center/lower left, the flat thin slightly powdery looking growth is what you are looking for. That thick bright white growth is certainly a contaminant.
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A.k.a
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Re: Advice on growing P. papuana [Re: inski]
#26794925 - 06/30/20 04:12 PM (3 years, 6 months ago) |
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Have you posted this in the exotics thread?? Never heard of this species before, always cool to see new stuff.
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Moopers
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Re: Advice on growing P. papuana [Re: inski]
#26795185 - 06/30/20 06:27 PM (3 years, 6 months ago) |
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Quote:
inski said: Make lots of transfers from the streak at the center/lower left, the flat thin slightly powdery looking growth is what you are looking for. That thick bright white growth is certainly a contaminant.
Thanks for chiming in! I might have wasted a lot of effort otherwise.
Since this post, the white areas are starting to turn tan/olive and the thick stuff is starting to turn green (Penicillium?). I may need to start fresh, unless you think any of this looks promising. If so, I'll take some transfers tonight and probably start another set of plates anyway.
Apologies for condensation.
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Moopers
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Re: Advice on growing P. papuana [Re: A.k.a]
#26795202 - 06/30/20 06:35 PM (3 years, 6 months ago) |
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Quote:
A.k.a said: Have you posted this in the exotics thread?? Never heard of this species before, always cool to see new stuff.
It's a beautifully strange-looking species, and Heyowana says they're quite potent, so when I saw a print for sale, I grabbed it.
I thought I'd start a separate thread on the species because the exotics thread and the P. tasmania/alutacea/papuana thread both are crowded with discussions about multiple species that make it hard for me to follow. Plus, this way I can track my own progress in one place.
I promise I'll post to the Exotics thread once I have something better to show than three plates full of contam.
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inski
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Re: Advice on growing P. papuana [Re: Moopers]
#26795993 - 07/01/20 12:19 AM (3 years, 6 months ago) |
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I would start again as it looks like the mold is sporulating and that will make it difficult to isolate any clean growth, if it's all you have to work with try isolating from that first dish.
The mycelium from P. papuana produces some tan to yellow pigmentation in culture as it matures so don't be discouraged by that.
Wish I saved prints from my experiment
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Moopers
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Re: Advice on growing P. papuana [Re: inski]
#26797241 - 07/01/20 03:04 PM (3 years, 6 months ago) |
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Quote:
inski said: I would start again as it looks like the mold is sporulating and that will make it difficult to isolate any clean growth, if it's all you have to work with try isolating from that first dish.
I made transfers from those plates and started 4 new plates. Fingers crossed!
Quote:
The mycelium from P. papuana produces some tan to yellow pigmentation in culture as it matures so don't be discouraged by that.
Good to know for these fresh plates. Thanks!
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Moopers
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Re: Advice on growing P. papuana [Re: Moopers]
#26807360 - 07/06/20 03:46 PM (3 years, 6 months ago) |
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I took Inski's direction to transfer from the powdery growth on those contaminated plates, and here's what I have so far. Looking good?
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Roger Clemency
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Re: Advice on growing P. papuana [Re: Moopers]
#26807471 - 07/06/20 04:46 PM (3 years, 6 months ago) |
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Yeah those all look promising. Except maybe the couple with satellite spots around them. I'd wait until they grow out just a little more and look for the most organized growth.
I can't wait to see what pops up from these
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Moopers
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Quote:
Roger Clemency said: Yeah those all look promising. Except maybe the couple with satellite spots around them.
Thanks for the input!
The satellite spots are probably from me scraping the agar trying to get the transfer off the scalpel.
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inski
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Re: Advice on growing P. papuana [Re: Moopers]
#26808126 - 07/06/20 11:58 PM (3 years, 6 months ago) |
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Quote:
Moopers said: The satellite spots are probably from me scraping the agar trying to get the transfer off the scalpel. 
I agree, that's exactly what it looks like, I've seen it many times.
These look good Moopers, is that black food colouring in your agar? If so I discourage the use of any food colouring in agar used for fungal cultures as it contains anti fungal and anti bacterial chemicals, if the purpose of using it was for visibility I suggest making MEA and pouring it as thin as possible, that way you can shine a light through from the back of the plate and look at the mycelium with a 20x hand lens.
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Roger Clemency
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Re: Advice on growing P. papuana [Re: inski]
#26808364 - 07/07/20 06:41 AM (3 years, 6 months ago) |
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Yeah I figured that’s what it was from. I have it happen as well but I never trust those spots still lol.
I think those are charcoal plates. I never liked food coloring for my plates. They looked cool but it actually made it more difficult for me to judge iffy plates. Those black plates though seem legit.
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Moopers
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Re: Advice on growing P. papuana [Re: inski]
#26810166 - 07/08/20 01:42 AM (3 years, 6 months ago) |
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Quote:
inski said: These look good Moopers
Thanks! I am glad to read that. You mentioned that some color change to tan is normal, yes? I'll post a pic tomorrow, but I think I'm already seeing some tanning.
Quote:
inski said: is that black food colouring in your agar?
Quote:
Roger Clemency said: I think those are charcoal plates.
They are carbon plates, not food coloring. I find it helps a lot with visibility. I buy prepoured plates because I am already stretched for time and running out of space for this hobby. I may inquire about getting custom tea agar done for cleaning up transfers/clones. I've seen a few posts on here saying it can be useful. Thoughts?
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Moopers
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Re: Advice on growing P. papuana [Re: Moopers]
#26819844 - 07/12/20 04:47 PM (3 years, 6 months ago) |
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Got busy and didn't take transfers until today. I used a jeweler's loop to try to find strandy growth on the edges, because every colony still looked powdery. As mentioned, pretty much every colony's myc is turning tan with time.

My fresh plates, 2 were overrun with Penicillium, one has one spot and one so far has none. If any colonies look promising I'll take more transfers.
Any feedback appreciated, as always!
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Moopers
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Re: Advice on growing P. papuana [Re: Moopers]
#26837333 - 07/21/20 11:48 PM (3 years, 6 months ago) |
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My last set of transfers aren't showing much in the way of strandy, organized growth. The two thicker colonies in the plate have the most organization.

The other colonies on the remaining plates are pretty chaotic looking under the jeweler's loupe, even though from higher up they look like they're "reaching out". They're all turning a tan color.
Should I keep taking transfers until a colony grows that looks like classic radial strandy myc growth?
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inski
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Re: Advice on growing P. papuana [Re: Moopers]
#26837343 - 07/21/20 11:57 PM (3 years, 6 months ago) |
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these look pretty typical of that species, I would make one more transfer from the top culture and the bottom culture on that plate onto individual plates and if they look clean use them to inoculate grain. Good luck
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Moopers
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Re: Advice on growing P. papuana [Re: inski]
#26837397 - 07/22/20 12:59 AM (3 years, 6 months ago) |
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Quote:
inski said: these look pretty typical of that species, I would make one more transfer from the top culture and the bottom culture on that plate onto individual plates and if they look clean use them to inoculate grain. Good luck
Thank you! Will do. For grain spawn sub, I think I'm going to boil a big handful of wood chips in with the grain, so there's some wood nutrition for it to munch on. I am assuming the species is wood-loving given that Heyowana found it near where logs were sawn.
Any tips for substrate and fruiting temps? Is *P. papuana* only suitable for an outdoor/balcony grow for fall fruiting, like other wood-lovers, or can I do this inside?
For bulk sub ingredients, I have:
- HWFP (apple-maple blend)
- HWFP (blend of alder, apple, hickory, maple, mesquite, other)
- Hickory wood chips
- Shaved aspen pet bedding
- Spent tea leaves
- Potting soil
- Powdered clay
In addition to the usuals (coir, peat, verm, perlite, gypsum, CaCO3, bran).
I'm unsure if I should be doing pasteurized supplemented sawdust like gourmets (but with a little added clay?), or an alkaline wood chip tek like for azures/cyanescens, or adding clay to potting soil and pasteurizing that, etc.
Thoughts, suggestions...?
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Moopers
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Re: Advice on growing P. papuana [Re: Moopers]
#26894349 - 08/22/20 09:35 PM (3 years, 5 months ago) |
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Still growing. Here's the plate grown out. I took a chunk to make LC and used most of the rest of this plate to inoculate some jars of oats. Will update as I go along.
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Moopers
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Re: Advice on growing P. papuana [Re: Moopers]
#26948726 - 09/22/20 01:21 AM (3 years, 4 months ago) |
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I wanted to share some photos of Psilocybe papuana on spawning substrate, cuz I haven't seen many photos yet.
This jar is one colony inoculated via LC into cakes of the Macmerdin's pan-cakes recipe. Instead of commercial manure compost, I used a local manure compost blend of sawdust and cow manure.
The mycelium is wispy but then forms spots. At points you can see filaments forming between the spots, like veins. I see these same filaments on some of my old agar plates of it.
You can also see (in person, not as obvious in the pic) that the myc of this colony is that creamy olive green on my agar plates.
This is a different colony inoculated via LC to hull-on oats. You can really see the filaments here.
This is a third colony inoculated via agar wedge to oat groats and ground spent tea leaves. It also has that creamy olive green color, spotty/dusty growth, and occasional filaments.
 
I also inoculated some brown rice in vitro pods (a la violet) with agar wedges and used LC to inoculate PF cakes (using ground wheat bran). I'll post more as those come along. I'll also post my old agar plates, which show some interesting color variation and weird spotting.
Edited by Moopers (09/22/20 11:40 AM)
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inski
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Re: Advice on growing P. papuana [Re: Moopers]
#26949274 - 09/22/20 12:35 PM (3 years, 4 months ago) |
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Here's some for comparison.
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Celestialexplorer1


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Re: Advice on growing P. papuana [Re: inski]
#26949430 - 09/22/20 02:10 PM (3 years, 4 months ago) |
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here’s also some
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Moopers
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Quote:
inski said: Here's some for comparison.

Quote:
Celestialexplorer1 said:
here’s also some
Inski's looks like my oat jar. Celestial, yours looks quite thick and happy. That's 20+ days after inoculation? Did you shake it at 20-30%?
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Celestialexplorer1


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Re: Advice on growing P. papuana [Re: Moopers]
#26949580 - 09/22/20 03:21 PM (3 years, 4 months ago) |
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The picture is old I spawned it quite a bit ago. It took 9 days till 100% the LC I made is fast af. I have another I nocced 5 days ago almost done. No shake.
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Moopers
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Quote:
Celestialexplorer1 said: The picture is old I spawned it quite a bit ago. It took 9 days till 100% the LC I made is fast af. I have another I nocced 5 days ago almost done. No shake.
Sounds like winner. I hope it fruits well for you and you can take prints to share .
Viridis said papuana fruits easily for him. Seems like this species might be domesticated soon.
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Celestialexplorer1


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Re: Advice on growing P. papuana [Re: Moopers]
#26969167 - 10/04/20 02:06 PM (3 years, 3 months ago) |
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 Papuana recovery tray day 2 from bulk bag. Also made a mini mono with the spawn that is about 60 or so %. After doing both I definitely would choose bulk bags over spawn and have another bulk bag doing really good. They are fast enough on the bulk bag it doesn’t make sense to do spawn.
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Moopers
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Quote:
Celestialexplorer1 said: Papuana recovery tray day 2 from bulk bag. Also made a mini mono with the spawn that is about 60 or so %. After doing both I definitely would choose bulk bags over spawn and have another bulk bag doing really good. They are fast enough on the bulk bag it doesn’t make sense to do spawn.
What's in your bulk bag mix?
Edited by Moopers (10/04/20 06:57 PM)
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Celestialexplorer1


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Re: Advice on growing P. papuana [Re: Moopers]
#26969677 - 10/04/20 07:04 PM (3 years, 3 months ago) |
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Jiffy/humus compost/verm/sand/hpoo/wheat bran (as nute supplement)
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Moopers
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Quote:
Celestialexplorer1 said: Jiffy/humus compost/verm/sand/hpoo/wheat bran (as nute supplement)
Interesting. So far the fastest sub for me has been verm+wheat bran PF-style cakes. Maybe it being a "clay-loving" species means it prefers denser or more mineral substrates.
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Celestialexplorer1


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Re: Advice on growing P. papuana [Re: Moopers]
#26969759 - 10/04/20 07:58 PM (3 years, 3 months ago) |
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Well on mushroom observer it says it was found in red granite soil I believe where wood had been sawn for years. I did not implement and wood into the substrate really only because I read that after I made it. For the next I may throw some alder pellets or fur bark chips into the mix.
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bobwastaken
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In my experience hunting wild P.papuana I've found this species fruits most prolifically from well rotted wood or forest humus forming large dense clusters. I've also found it fruiting from red clay but usually solitaire or in groups.







I'd imagine a mix of quality potting soil and wood mulch should serve as a great bulk substrate but there may be many combinations which prove favorable.
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Moopers
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Quote:
Celestialexplorer1 said: Well on mushroom observer it says it was found in red granite soil I believe where wood had been sawn for years. I did not implement and wood into the substrate really only because I read that after I made it. For the next I may throw some alder pellets or fur bark chips into the mix.
I'll have to try a woodier substrate, too.
For your bulk bags are you pasteurizing them and inoculating with grain spawn? Or sterilizing and using LC directly?
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Moopers
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Re: Advice on growing P. papuana [Re: bobwastaken]
#26969870 - 10/04/20 09:44 PM (3 years, 3 months ago) |
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Quote:
bobwastaken said: In my experience hunting wild P.papuana I've found this species fruits most prolifically from well rotted wood or forest humus forming large dense clusters. I've also found it fruiting from red clay but usually solitaire or in groups.
I'd imagine a mix of quality potting soil and wood mulch should serve as a great bulk substrate but there may be many combinations which prove favorable.
In what temps do you tend to find them?
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bobwastaken
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Re: Advice on growing P. papuana [Re: Moopers]
#26969885 - 10/04/20 10:00 PM (3 years, 3 months ago) |
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It depends on the location. I've found them at near sea level and high altitude (1300m+). P.subaeruginosa can be found growing in the vicinity of P.papuana suggesting they do well in temperate climates. Those near sea level were growing during the tropical summer season among P.cubensis, Pan cyanescens. I'd say they're not especially fussy regarding temps. Room temp should suffice. It may be worth mentioning the species is also capable of surviving a prolonged freeze where they occur at high altitude.
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Moopers
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Re: Advice on growing P. papuana [Re: bobwastaken]
#26969933 - 10/04/20 10:43 PM (3 years, 3 months ago) |
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Super helpful, thank you!
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Celestialexplorer1


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Re: Advice on growing P. papuana [Re: Moopers]
#26969980 - 10/04/20 11:57 PM (3 years, 3 months ago) |
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Sterilize 3-4 hrs I use wheat bran for the nutes. Then LC direct.
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Celestialexplorer1


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Hopefully my tray still fruits without the wood. It looks pretty healthy and recovers super quick I think I’ll be alright but definitely logging this in my brain for the next run. Used up all the LC and have two bulk bags and a mini mono which was spawn and pastuerized sub(doing well none the less)
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A.k.a
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Those pictures are great.
I can’t imagine living somewhere that so many actives grow.
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Moopers
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Re: Advice on growing P. papuana [Re: A.k.a]
#26985725 - 10/14/20 06:17 PM (3 years, 3 months ago) |
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Think I should put these into fruiting?
They've got veins like a bodybuilder!
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Celestialexplorer1


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Re: Advice on growing P. papuana [Re: Moopers]
#26985745 - 10/14/20 06:23 PM (3 years, 3 months ago) |
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Here’s what the tray I just cased ten mins ago looked like. I did a thicker casing on this tray and I’m gonna let that sit for a couple days before putting into fruiting.
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Edited by Celestialexplorer1 (10/14/20 06:24 PM)
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Moopers
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Quote:
Celestialexplorer1 said:
Here’s what the tray I just cased ten mins ago looked like. I did a thicker casing on this tray and I’m gonna let that sit for a couple days before putting into fruiting.
Looks good, I think. I have some violet-style brown rice in vitro quarts that look similar, light mycelium with a few thicker spots. They'll be ready to case in a little over a week.
What do you use for casing?
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Moopers
Pan noob


Registered: 05/08/20
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Re: Advice on growing P. papuana [Re: Moopers]
#26986107 - 10/14/20 10:02 PM (3 years, 3 months ago) |
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I was thinking peat, verm and clay, maybe 40/40/20 and some CaCO3.
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Celestialexplorer1


Registered: 03/25/20
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Re: Advice on growing P. papuana [Re: Moopers]
#26986502 - 10/15/20 06:37 AM (3 years, 3 months ago) |
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Jiffy/verm/sand/gypsum
Mushroom observer says I was found growing in red granite soil next to a pile of sawn logs and logs had been spawn there for years.
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Edited by Celestialexplorer1 (10/15/20 06:41 AM)
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bobwastaken
under construction


Registered: 06/17/10
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Loc: SA
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My P.papuana are beginning to fruit. Only a couple early risers however I'm seeing a lot of primordia forming beneath the casing. substrate is wheat>coir and cased with untreated potting soil. These trays have been in fruiting conditions for near 2 months.
  In the meantime I've been working with this species on agar and after going through hundreds of plates I've found 2 cultures which will produce primordia on agar in minimal time. Since Viridis420 was able to fruit the species in a week I'd suspected there may be some fast fruiting strains to be found.
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Celestialexplorer1



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Re: Advice on growing P. papuana [Re: bobwastaken]
#27086497 - 12/12/20 11:02 PM (3 years, 1 month ago) |
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That is so amazing. I’m still trying. Just went back to my original spore plate a couple days ago. Great job man I love that black cap.
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 To spend just one moment in eternity
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bobwastaken
under construction


Registered: 06/17/10
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Loc: SA
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Thanks and yes I also love the black capped phenotype. As long as the mycelium is healthy I think you're in with a fair chance of seeing fruits. I wish I lived closer to where they occur naturally so I could clone and test wild specimens.
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Celestialexplorer1



Registered: 03/25/20
Posts: 1,803
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Re: Advice on growing P. papuana [Re: bobwastaken]
#27086907 - 12/13/20 08:35 AM (3 years, 1 month ago) |
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What are your temps? Also what kinda surface conditions are you keeping. I just pawned some Papuana the other day
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 To spend just one moment in eternity
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mycorry
The Empirical



Registered: 06/01/18
Posts: 385
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Re: Advice on growing P. papuana [Re: bobwastaken]
#27097960 - 12/19/20 03:18 PM (3 years, 1 month ago) |
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Quote:
bobwastaken said: My P.papuana are beginning to fruit. Only a couple early risers however I'm seeing a lot of primordia forming beneath the casing. substrate is wheat>coir and cased with untreated potting soil. These trays have been in fruiting conditions for near 2 months.
  In the meantime I've been working with this species on agar and after going through hundreds of plates I've found 2 cultures which will produce primordia on agar in minimal time. Since Viridis420 was able to fruit the species in a week I'd suspected there may be some fast fruiting strains to be found.
Amazing work from bobwastaken, as usual!
Very impressive stuff. I've fruited paups a few times now but they all seem to be the basic caramel colored cap. I am really looking for that black phenotype but no luck yet. Also, the few times I have fruited paups they certainly didn't look as healthy as your pics! Look at that shag!
Potting soil casing! It seems like everyone gets really good results with this stuff but me. I suspect that it could just be the specific recipe I was using.
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Edited by mycorry (12/19/20 03:21 PM)
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bobwastaken
under construction


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Loc: SA
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Re: Advice on growing P. papuana [Re: mycorry]
#27282176 - 04/26/21 02:52 AM (2 years, 8 months ago) |
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Thanks Mycorry
A couple test dishes of P.papuana are pinning. Method was simply selecting a number of sectors from MS and allowing plates to grow out before applying a thin casing of coir/manure. Of 50 cultures two have fruited so far.
The first being a black colored phenotype. Unfortunately it isn't sporulating.




Another culture has produced a striking blue fruit. This specimen is sporulating. Both types will be cloned and bulk fruiting attempts undertaken.
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Dart King
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Re: Advice on growing P. papuana [Re: bobwastaken]
#27282284 - 04/26/21 05:53 AM (2 years, 8 months ago) |
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That's sick
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Awaken_Us
Stranger
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Re: Advice on growing P. papuana [Re: Dart King]
#27307944 - 05/14/21 07:08 AM (2 years, 8 months ago) |
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amazing thread, beautiful photos thanks for sharing. I have what we believe to be papuana from New Zealand on agar and will be making LC soon.
Thanks for the tips
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