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OfflineMoopers
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Registered: 05/08/20
Posts: 525
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Re: Advice on growing P. papuana [Re: inski]
    #26810166 - 07/08/20 01:42 AM (3 years, 6 months ago)

Quote:

inski said:
These look good Moopers



Thanks! I am glad to read that. You mentioned that some color change to tan is normal, yes? I'll post a pic tomorrow, but I think I'm already seeing some tanning.

Quote:

inski said:
is that black food colouring in your agar?



Quote:

Roger Clemency said:
I think those are charcoal plates.



They are carbon plates, not food coloring. I find it helps a lot with visibility. I buy prepoured plates because I am already stretched for time and running out of space for this hobby. I may inquire about getting custom tea agar done for cleaning up transfers/clones. I've seen a few posts on here saying it can be useful. Thoughts?


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OfflineMoopers
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Registered: 05/08/20
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Re: Advice on growing P. papuana [Re: Moopers]
    #26819844 - 07/12/20 04:47 PM (3 years, 6 months ago)

Got busy and didn't take transfers until today. I used a jeweler's loop to try to find strandy growth on the edges, because every colony still looked powdery. As mentioned, pretty much every colony's myc is turning tan with time.



My fresh plates, 2 were overrun with Penicillium, one has one spot and one so far has none. If any colonies look promising I'll take more transfers.

Any feedback appreciated, as always!


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Moopers]
    #26837333 - 07/21/20 11:48 PM (3 years, 6 months ago)

My last set of transfers aren't showing much in the way of strandy, organized growth. The two thicker colonies in the plate have the most organization.



The other colonies on the remaining plates are pretty chaotic looking under the jeweler's loupe, even though from higher up they look like they're "reaching out". They're all turning a tan color.

Should I keep taking transfers until a colony grows that looks like classic radial strandy myc growth?


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InvisibleinskiM
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Re: Advice on growing P. papuana [Re: Moopers]
    #26837343 - 07/21/20 11:57 PM (3 years, 6 months ago)

these look pretty typical of that species, I would make one more transfer from the top culture and the bottom culture on that plate onto individual plates and if they look clean use them to inoculate grain.
Good luck:thumbup:


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: inski]
    #26837397 - 07/22/20 12:59 AM (3 years, 6 months ago)

Quote:

inski said:
these look pretty typical of that species, I would make one more transfer from the top culture and the bottom culture on that plate onto individual plates and if they look clean use them to inoculate grain.
Good luck:thumbup:




Thank you! Will do. For grain spawn sub, I think I'm going to boil a big handful of wood chips in with the grain, so there's some wood nutrition for it to munch on. I am assuming the species is wood-loving given that Heyowana found it near where logs were sawn.

Any tips for substrate and fruiting temps? Is *P. papuana* only suitable for an outdoor/balcony grow for fall fruiting, like other wood-lovers, or can I do this inside?

For bulk sub ingredients, I have:
  • HWFP (apple-maple blend)
  • HWFP (blend of alder, apple, hickory, maple, mesquite, other)
  • Hickory wood chips
  • Shaved aspen pet bedding
  • Spent tea leaves
  • Potting soil
  • Powdered clay

In addition to the usuals (coir, peat, verm, perlite, gypsum, CaCO3, bran).

I'm unsure if I should be doing pasteurized supplemented sawdust like gourmets (but with a little added clay?), or an alkaline wood chip tek like for azures/cyanescens, or adding clay to potting soil and pasteurizing that, etc.

Thoughts, suggestions...?


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Moopers]
    #26894349 - 08/22/20 09:35 PM (3 years, 5 months ago)

Still growing. Here's the plate grown out. I took a chunk to make LC and used most of the rest of this plate to inoculate some jars of oats. Will update as I go along.



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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Moopers]
    #26948726 - 09/22/20 01:21 AM (3 years, 4 months ago)

I wanted to share some photos of Psilocybe papuana on spawning substrate, cuz I haven't seen many photos yet.

This jar is one colony inoculated via LC into cakes of the Macmerdin's pan-cakes recipe. Instead of commercial manure compost, I used a local manure compost blend of sawdust and cow manure.

The mycelium is wispy but then forms spots. At points you can see filaments forming between the spots, like veins. I see these same filaments on some of my old agar plates of it.

You can also see (in person, not as obvious in the pic) that the myc of this colony is that creamy olive green on my agar plates.



This is a different colony inoculated via LC to hull-on oats. You can really see the filaments here.



This is a third colony inoculated via agar wedge to oat groats and ground spent tea leaves. It also has that creamy olive green color, spotty/dusty growth, and occasional filaments.


I also inoculated some brown rice in vitro pods (a la violet) with agar wedges and used LC to inoculate PF cakes (using ground wheat bran). I'll post more as those come along. I'll also post my old agar plates, which show some interesting color variation and weird spotting.


Edited by Moopers (09/22/20 11:40 AM)


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InvisibleinskiM
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Re: Advice on growing P. papuana [Re: Moopers]
    #26949274 - 09/22/20 12:35 PM (3 years, 4 months ago)

Here's some for comparison.


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OfflineCelestialexplorer1
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Re: Advice on growing P. papuana [Re: inski]
    #26949430 - 09/22/20 02:10 PM (3 years, 4 months ago)

here’s also some


--------------------


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Celestialexplorer1]
    #26949502 - 09/22/20 02:42 PM (3 years, 4 months ago)

Quote:

inski said:
Here's some for comparison.





Quote:

Celestialexplorer1 said:
here’s also some




Inski's looks like my oat jar. Celestial, yours looks quite  thick and happy. That's 20+ days after inoculation? Did you shake it at 20-30%?


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OfflineCelestialexplorer1
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Re: Advice on growing P. papuana [Re: Moopers]
    #26949580 - 09/22/20 03:21 PM (3 years, 4 months ago)

The picture is old
I spawned it quite a bit ago. It took 9 days till 100% the LC I made is fast af. I have another I nocced 5 days ago almost done. No shake.


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Celestialexplorer1]
    #26949670 - 09/22/20 04:07 PM (3 years, 4 months ago)

Quote:

Celestialexplorer1 said:
The picture is old
I spawned it quite a bit ago. It took 9 days till 100% the LC I made is fast af. I have another I nocced 5 days ago almost done. No shake.




Sounds like winner. I hope it fruits well for you and you can take prints to share :sporedrop:.

Viridis said papuana fruits easily for him. Seems like this species might be domesticated soon.


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OfflineCelestialexplorer1
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Re: Advice on growing P. papuana [Re: Moopers]
    #26969167 - 10/04/20 02:06 PM (3 years, 3 months ago)


Papuana recovery tray day 2 from bulk bag.
Also made a mini mono with the spawn that is about 60 or so %.
After doing both I definitely would choose bulk bags over spawn and have another bulk bag doing really good. They are fast enough on the bulk bag it doesn’t make sense to do spawn.


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Celestialexplorer1]
    #26969666 - 10/04/20 06:57 PM (3 years, 3 months ago)

Quote:

Celestialexplorer1 said:
Papuana recovery tray day 2 from bulk bag.
Also made a mini mono with the spawn that is about 60 or so %.
After doing both I definitely would choose bulk bags over spawn and have another bulk bag doing really good. They are fast enough on the bulk bag it doesn’t make sense to do spawn.




What's in your bulk bag mix?


Edited by Moopers (10/04/20 06:57 PM)


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OfflineCelestialexplorer1
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Re: Advice on growing P. papuana [Re: Moopers]
    #26969677 - 10/04/20 07:04 PM (3 years, 3 months ago)

Jiffy/humus compost/verm/sand/hpoo/wheat bran (as nute supplement)


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OfflineMoopers
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Posts: 525
Last seen: 1 month, 26 days
Re: Advice on growing P. papuana [Re: Celestialexplorer1]
    #26969744 - 10/04/20 07:44 PM (3 years, 3 months ago)

Quote:

Celestialexplorer1 said:
Jiffy/humus compost/verm/sand/hpoo/wheat bran (as nute supplement)




Interesting. So far the fastest sub for me has been verm+wheat bran PF-style cakes. Maybe it being a "clay-loving" species means it prefers denser or more mineral substrates.


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OfflineCelestialexplorer1
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Re: Advice on growing P. papuana [Re: Moopers]
    #26969759 - 10/04/20 07:58 PM (3 years, 3 months ago)

Well on mushroom observer it says it was found in red granite soil I believe where wood had been sawn for years. I did not implement and wood into the substrate really only because I read that after I made it. For the next I may throw some alder pellets or fur bark chips into the mix.


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InvisiblebobwastakenS
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Re: Advice on growing P. papuana [Re: Celestialexplorer1]
    #26969830 - 10/04/20 08:53 PM (3 years, 3 months ago)

In my experience hunting wild P.papuana I've found this species fruits most prolifically from well rotted wood or forest humus forming large dense clusters. I've also found it fruiting from red clay but usually solitaire or in groups.










I'd imagine a mix of quality potting soil and wood mulch should serve as a great bulk substrate but there may be many combinations which prove favorable.


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OfflineMoopers
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Registered: 05/08/20
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Re: Advice on growing P. papuana [Re: Celestialexplorer1]
    #26969869 - 10/04/20 09:43 PM (3 years, 3 months ago)

Quote:

Celestialexplorer1 said:
Well on mushroom observer it says it was found in red granite soil I believe where wood had been sawn for years. I did not implement and wood into the substrate really only because I read that after I made it. For the next I may throw some alder pellets or fur bark chips into the mix.




I'll have to try a woodier substrate, too.

For your bulk bags are you pasteurizing them and inoculating with grain spawn? Or sterilizing and using LC directly?


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: bobwastaken]
    #26969870 - 10/04/20 09:44 PM (3 years, 3 months ago)

Quote:

bobwastaken said:
In my experience hunting wild P.papuana I've found this species fruits most prolifically from well rotted wood or forest humus forming large dense clusters. I've also found it fruiting from red clay but usually solitaire or in groups.

I'd imagine a mix of quality potting soil and wood mulch should serve as a great bulk substrate but there may be many combinations which prove favorable.




In what temps do you tend to find them?


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