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OfflineMoopers
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Growing P. papuana
    #26752707 - 06/17/20 08:15 PM (3 years, 7 months ago)

I purchased a print of Psilocybe papuana, and I'm hoping to grow it out. I understand I'm in mostly uncharted territory here, with only a few folks on shroomery growing this species. I think it would be great to consolidate knowledge, now that a vendor currently has prints for sale. Anyone else growing this?

I would love any guidance or knowledge you all can share. I've sent a PM to inski to inquire about his methods, and I'll be very thankful if we wants to/has time to write them up. I thought I'd put it out there for anyone else who is growing or has grown P. papuana. Any advice on what works and doesn't?

It's been described as "clay-loving", rather than as coprophilous or lignicolous. It's been recently found in NSW Australia by Heyowana, growing in clay soil near where logs were sawn year after year (Eucalyptus spp.). Given this, is it more of a lignicolous species? I believe it also fruits best in fall?

I plan to put spore print to agar, agar to grain (brown rice or rye). I have some ideas to experiment from there, but want to know what's been done/what worked/what failed/etc.


Edited by Moopers (06/20/20 07:28 PM)


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Moopers]
    #26757107 - 06/19/20 11:21 AM (3 years, 7 months ago)

Put spores to MYA yesterday, 4 plates. I'll post pics if/when they show germination.


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Offlineexzile
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Re: Advice on growing P. papuana [Re: Moopers]
    #26757199 - 06/19/20 11:55 AM (3 years, 7 months ago)

Following.


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OfflineCelestialexplorer1
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Re: Advice on growing P. papuana [Re: Moopers]
    #26757203 - 06/19/20 11:56 AM (3 years, 7 months ago)

Don’t know much about them. There’s really next to no information on them on the web. I’m definitely interested in knowing more though.


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Offlinemushpunx
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Re: Advice on growing P. papuana [Re: Celestialexplorer1]
    #26757506 - 06/19/20 01:44 PM (3 years, 7 months ago)

These are seriously cool looking!
My first time seeing these.

Following!


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: mushpunx]
    #26759686 - 06/20/20 11:33 AM (3 years, 7 months ago)

Germination on 2 plates after 2 days, not bad. Plates were kept 71-74F. The haze is condensation.



Edited by Moopers (06/20/20 03:07 PM)


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Moopers]
    #26793625 - 06/30/20 02:28 AM (3 years, 6 months ago)

What started so clean looking is now quite a mess. Truly a spore print done in the wild. It's going to take a few transfers before these first cultures are useable.

The P. papuana myc is thick and bright, like cream. Three plates had usable cultures. The fourth plate was all contam along the inoculation zigzag.



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InvisibleNobler Hino
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Re: Advice on growing P. papuana [Re: Moopers]
    #26794310 - 06/30/20 11:08 AM (3 years, 6 months ago)

That flat growth reminds me of tamp mycelium. I would say you have some nice areas to transfer from.

Just noticed where you cut, that thick shit is what I would avoid.


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"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


Edited by Nobler Hino (06/30/20 11:09 AM)


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Nobler Hino]
    #26794326 - 06/30/20 11:21 AM (3 years, 6 months ago)

Quote:

Nobler Hino said:
That flat growth reminds me of tamp mycelium. I would say you have some nice areas to transfer from.

Just noticed where you cut, that thick shit is what I would avoid.




What's wrong with the thick stuff? Those are the spots that first germinated, and I was avoiding the flat faint/powdery white areas because there's so much discoloration I assume is contam. That was my reasoning, but I'm happy to be educated if I chose poorly.

Where would you suggest? I can always take more transfers.


Edited by Moopers (06/30/20 11:25 AM)


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InvisibleinskiM
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Re: Advice on growing P. papuana [Re: Moopers]
    #26794461 - 06/30/20 12:37 PM (3 years, 6 months ago)

Make lots of transfers from the streak at the center/lower left, the flat thin slightly powdery looking growth is what you are looking for.
That thick bright white growth is certainly a contaminant.


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OfflineA.k.aM
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Re: Advice on growing P. papuana [Re: inski]
    #26794925 - 06/30/20 04:12 PM (3 years, 6 months ago)

Have you posted this in the exotics thread?? Never heard of this species before, always cool to see new stuff.


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: inski]
    #26795185 - 06/30/20 06:27 PM (3 years, 6 months ago)

Quote:

inski said:
Make lots of transfers from the streak at the center/lower left, the flat thin slightly powdery looking growth is what you are looking for.
That thick bright white growth is certainly a contaminant.




Thanks for chiming in! I might have wasted a lot of effort otherwise.

Since this post, the white areas are starting to turn tan/olive and the thick stuff is starting to turn green (Penicillium?). I may need to start fresh, unless you think any of this looks promising. If so, I'll take some transfers tonight and probably start another set of plates anyway.

Apologies for condensation.



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OfflineMoopers
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Re: Advice on growing P. papuana [Re: A.k.a]
    #26795202 - 06/30/20 06:35 PM (3 years, 6 months ago)

Quote:

A.k.a said:
Have you posted this in the exotics thread?? Never heard of this species before, always cool to see new stuff.




It's a beautifully strange-looking species, and Heyowana says they're quite potent, so when I saw a print for sale, I grabbed it.

I thought I'd start a separate thread on the species because the exotics thread and the P. tasmania/alutacea/papuana thread both are crowded with discussions about multiple species that make it hard for me to follow. Plus, this way I can track my own progress in one place.

I promise I'll post to the Exotics thread once I have something better to show than three plates full of contam. :laugh2:


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InvisibleinskiM
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Re: Advice on growing P. papuana [Re: Moopers]
    #26795993 - 07/01/20 12:19 AM (3 years, 6 months ago)

I would start again as it looks like the mold is sporulating and that will make it difficult to isolate any clean growth, if it's all you have to work with try isolating from that first dish.

The mycelium from P. papuana produces some tan to yellow pigmentation in culture as it matures so don't be discouraged by that.

Wish I saved prints from my experiment:shrug:


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: inski]
    #26797241 - 07/01/20 03:04 PM (3 years, 6 months ago)

Quote:

inski said:
I would start again as it looks like the mold is sporulating and that will make it difficult to isolate any clean growth, if it's all you have to work with try isolating from that first dish.




I made transfers from those plates and started 4 new plates. Fingers crossed!

Quote:

The mycelium from P. papuana produces some tan to yellow pigmentation in culture as it matures so don't be discouraged by that.




Good to know for these fresh plates. Thanks!


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Moopers]
    #26807360 - 07/06/20 03:46 PM (3 years, 6 months ago)

I took Inski's direction to transfer from the powdery growth on those contaminated plates, and here's what I have so far. Looking good?



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InvisibleRoger Clemency
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Re: Advice on growing P. papuana [Re: Moopers]
    #26807471 - 07/06/20 04:46 PM (3 years, 6 months ago)

Yeah those all look promising. Except maybe the couple with satellite spots around them. I'd wait until they grow out just a little more and look for the most organized growth.

I can't wait to see what pops up from these :anticipation:


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OfflineMoopers
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Re: Advice on growing P. papuana [Re: Roger Clemency]
    #26807952 - 07/06/20 09:16 PM (3 years, 6 months ago)

Quote:

Roger Clemency said:
Yeah those all look promising. Except maybe the couple with satellite spots around them.




Thanks for the input!

The satellite spots are probably from me scraping the agar trying to get the transfer off the scalpel.  :rofl:


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InvisibleinskiM
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Re: Advice on growing P. papuana [Re: Moopers]
    #26808126 - 07/06/20 11:58 PM (3 years, 6 months ago)

Quote:

Moopers said:
The satellite spots are probably from me scraping the agar trying to get the transfer off the scalpel.  :rofl:




I agree, that's exactly what it looks like, I've seen it many times.

These look good Moopers, is that black food colouring in your agar?
If so I discourage the use of any food colouring in agar used for fungal cultures as it contains anti fungal and anti bacterial chemicals, if the purpose of using it was for visibility I suggest making MEA and pouring it as thin as possible, that way you can shine a light through from the back of the plate and look at the mycelium with a 20x hand lens.


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InvisibleRoger Clemency
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Re: Advice on growing P. papuana [Re: inski]
    #26808364 - 07/07/20 06:41 AM (3 years, 6 months ago)

Yeah I figured that’s what it was from. I have it happen as well but I never trust those spots still lol.

I think those are charcoal plates. I never liked food coloring for my plates. They looked cool but it actually made it more difficult for me to judge iffy plates. Those black plates though seem legit.


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Heaven starts right where hell ends


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