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Offlinetowerbeats2020
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Registered: 05/12/20
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Orange Bacterial Contamination?
    #26752035 - 06/17/20 03:53 PM (3 years, 7 months ago)

Hey guys,

A couple weeks ago one of my jars showed this small orange spot in the mycelium. It hasn't grown at all since the 2 weeks I noticed it. It's almost hard to tell unless you look closely. Is this bacterial contamination? Think I should just toss this jar without opening? Could I birth it and just see what happens? This is BRF, inoculated with spore syringe using all sterile techniques, SAB, flame, alcohol, etc.

Note: the greenish yellow to the lower right of the orange i'm referring to is just light reflection on the glass jar.

Thank you!



Edited by towerbeats2020 (06/17/20 03:55 PM)


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Offlinemushpunx
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Registered: 04/20/14
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Re: Orange Bacterial Contamination? [Re: towerbeats2020]
    #26752067 - 06/17/20 04:12 PM (3 years, 7 months ago)

That looks like metabolites. These are produced in response to bacteria usually, so your cake could possibly have a little bacteria but I would fruit it, they will often do fine.

Next time don't leave tape over the holes, your substrate needs to breath. It is protected from contaminants by the layer of dry vermiculate that should be on the top of the cake, it acts as a filter.

When you inoculated, did you wipe the needle with alcohol *after* flame sterilizing? That is a common mistake people make, the alcohol contaminates the needle after it's been heat sterilized.

Is that cake fully colonized? If it is birth it.


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Offlinetowerbeats2020
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Re: Orange Bacterial Contamination? [Re: mushpunx]
    #26752101 - 06/17/20 04:27 PM (3 years, 7 months ago)

Hey man,

Thanks so much for the quick response!

I'm happy to hear you think it is metabolites, and not contamination. And you totally called it, I hadn't done enough research prior to inoculating, and the guide I was following said to quickly wipe the needle with alcohol after flame sterilizing in order to cool the needle -- so that's what I did.

After further reading I realized that is wrong, and the first bit of liquid is sufficient to cool the needle.

I believe it is fully colonized, it's all white and has pulled away from the jar. I was just giving it a little more time for the consolidation period. I will birth!

Thanks again for the help


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Offlinesmarterwater
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Registered: 06/17/20
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Re: Orange Bacterial Contamination? [Re: mushpunx]
    #26752194 - 06/17/20 04:58 PM (3 years, 7 months ago)

EDIT - sorry wrong post >_<


Edited by smarterwater (06/17/20 04:58 PM)


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