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OfflineAgarStudent
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Limitations on Agar
    #26750849 - 06/17/20 07:04 AM (3 years, 7 months ago)

Is it possible to keep transplanting pieces of agar in order to sustain a strain, or will this process fail?
My only question is I maintained the same strain for two years until eventually most of my grows went bad, jars looked blobby and trich took over.  Does opportunistic infection hide in mycelium without being seen eventually if you don’t make master slants?

Thanks so much for feedback on anyone who has had the same thing


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InvisibleinskiM
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Re: Limitations on Agar [Re: AgarStudent]
    #26751840 - 06/17/20 02:25 PM (3 years, 7 months ago)

At some stage over those two years you probably introduced the contamination, it's best to save a slant of the original clean culture to go back to.


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InvisibleCaps McGee
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Re: Limitations on Agar [Re: inski]
    #26751895 - 06/17/20 02:52 PM (3 years, 7 months ago)

Clean culture is clean until it's not:meaning any contamination seen was introduced... I dont know of any contaminants that truly hide, but bacteria can be difficult to spot if its meshed in: particularly with tomentose cultures... often times the only indication being a clear halo of growth that may or may not have topology and/or color... if your technique is stellar and your culture is in fact clean, it will remain as such... slants are wise,but not necessary... I've been running the same gourmet cultures, and a few select cubensis isolates, transfer to transfer for months, even a year plus in a few instances... I occasionally see satelite contaminant colonies, implying introduction due to subpar technique... rarely do I see meshed contamination, but typically from spore print or swab... post a picture of your plates, might be something you're missing


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OfflineAgarStudent
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Re: Limitations on Agar [Re: Caps McGee]
    #26751924 - 06/17/20 03:06 PM (3 years, 7 months ago)

Thanks caps -

That would be my culture-
They grow prolifically on agar and I have never seen any artefacts.



Any feedback appreciated...


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Why reinvent the wheel when someone here has already done the science?



Edited by AgarStudent (06/17/20 03:08 PM)


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InvisibleCaps McGee
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Re: Limitations on Agar [Re: AgarStudent]
    #26751940 - 06/17/20 03:12 PM (3 years, 7 months ago)

Any plates that aren't fully colonized? I cant see the leading edge on media to tell if there is underlying bacteria... a half colonized plate, or even one that's just starting to recover?

I will say I'm not too keen with the whispy, almost voidish growth closest to the wedge...  almost like the mycelium outran something just outside of the change in morphology


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OfflineAgarStudent
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Re: Limitations on Agar [Re: Caps McGee]
    #26752327 - 06/17/20 05:52 PM (3 years, 7 months ago)

Currently they are all colonised, but thanks for the insight.
I will have to do another transplant to rule this out.
Do you know what wispy growth could possibly be?  I have also seen this subtle growth in jars of mycelium? 

Cheers


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Why reinvent the wheel when someone here has already done the science?



Edited by AgarStudent (06/17/20 05:57 PM)


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OfflineCocaineBuffet
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Re: Limitations on Agar [Re: AgarStudent]
    #26752426 - 06/17/20 06:24 PM (3 years, 7 months ago)

If I was in your position I would either start from MS or wait to see if the plates pin and start taking transfers from the clone. You could take transfers from different parts of the plate and compare how they grow but if they all end up looking the same I personally would feel like I am back at square 1.


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OfflineAgarStudent
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Re: Limitations on Agar [Re: CocaineBuffet]
    #26752514 - 06/17/20 06:53 PM (3 years, 7 months ago)

Thanks for that,

It is a different look from my ecuador that has a more ryhzomorphic appearance οΏ½οΏ½



Does this PE plate look better than the first possibly contaminated plate?
Thanks for the help.



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Why reinvent the wheel when someone here has already done the science?



Edited by AgarStudent (06/17/20 10:23 PM)


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InvisibleCaps McGee
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Re: Limitations on Agar [Re: AgarStudent]
    #26754634 - 06/18/20 03:05 PM (3 years, 7 months ago)

Morphology will change from one variety to another: tomentose is the fluffy growth, and rhizomorphic  is the ropey growth that resembles roots... I've seen clean plates of single origin display both types of growth... these plates make me feel like theres bacteria: you need to transfer from the leading edge before the plate fully colonizes so that if there is a sinister piggybacker, you have a better chance at getting away from it, as the mycelium typically outruns the contaminant initially plates that sit fully colonized, become fully colonized by the stowaway as well
:rollsafe:


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OfflineAgarStudent
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Re: Limitations on Agar [Re: Caps McGee]
    #26755610 - 06/18/20 08:48 PM (3 years, 7 months ago)

Thanks caps-

Does the Ecuador and PE look okay compared to the first plate?

Cheers


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