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SunFlare
Stranger

Registered: 10/13/17
Posts: 59
Last seen: 1 year, 10 months
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Rehydrating Spores - Emergency Revival after 8 years of Storage?
#26750771 - 06/17/20 05:54 AM (3 years, 7 months ago) |
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1.have some cube spore prints that are ~8 years old and tried to germinate them on agar without success... its been a month and 0% growth occurred.
2.loaded a steam sterilized spore syringe with a water/spore combination and let it hydrate for 7 days.
injected into PF cake and agar. no growth only white glibber which most likely is bacteria.
any advice on how to get the spore prints to germinate?
maybe LC with LME , then on agar?
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 14 days
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Re: Rehydrating Spores - Emergency Revival after 8 years of Storage? [Re: SunFlare]
#26750828 - 06/17/20 06:38 AM (3 years, 7 months ago) |
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What's your agar recipe? I find a softer agar helps sometimes for germinating spores. My normal is 20g agar per 1L of water. I havent done it in a while but if I'm remembering right I start off around 16-17 g agar per 1L, give or take for spores that wont germinate.
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SunFlare
Stranger

Registered: 10/13/17
Posts: 59
Last seen: 1 year, 10 months
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Re: Rehydrating Spores - Emergency Revival after 8 years of Storage? [Re: Gan]
#26750831 - 06/17/20 06:44 AM (3 years, 7 months ago) |
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Quote:
Gan said: What's your agar recipe? I find a softer agar helps sometimes for germinating spores. My normal is 20g agar per 1L of water. I havent done it in a while but if I'm remembering right I start off around 16-17 g agar per 1L, give or take for spores that wont germinate.
1 liter water 20g agar 15g light malt extract
c10's Agar Guide + Tips & Tricks (post #1000) https://www.shroomery.org/forums/showflat.php/Number/24330957/fpart/all/vc/1
by using 3-4g of less agar you think it will work?
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T666NY
From Mars


Registered: 09/29/19
Posts: 127
Last seen: 2 years, 1 month
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Re: Rehydrating Spores - Emergency Revival after 8 years of Storage? [Re: SunFlare]
#26750842 - 06/17/20 06:56 AM (3 years, 7 months ago) |
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Try different Agar recipes, maybe the LC. Spores should be viable for years. I’ve seen people successfully germinate 17-year-old spores.
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SunFlare
Stranger

Registered: 10/13/17
Posts: 59
Last seen: 1 year, 10 months
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Re: Rehydrating Spores - Emergency Revival after 8 years of Storage? [Re: T666NY]
#26750844 - 06/17/20 07:01 AM (3 years, 7 months ago) |
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Taken from TL's Simple Liquid Culture (LC) Tek (Recipe) Using Two Ingredients (ELME)
3g ELME per 600mL water sterilize this inside a jar. inject spore syringe into it.
let mycelium colonize the LC culture and transfer to agar?
https://www.shroomery.org/forums/showflat.php/Number/15234219/fpart/all/vc/1
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The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 13 days, 10 hours
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Re: Rehydrating Spores - Emergency Revival after 8 years of Storage? [Re: SunFlare]
#26750848 - 06/17/20 07:04 AM (3 years, 7 months ago) |
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Some people have had success forcing water into the spores by putting a rubber band on the plunger to increase the pressure inside the syringe.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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SunFlare
Stranger

Registered: 10/13/17
Posts: 59
Last seen: 1 year, 10 months
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Re: Rehydrating Spores - Emergency Revival after 8 years of Storage? [Re: The Mycologist]
#26800812 - 07/03/20 06:34 AM (3 years, 7 months ago) |
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Quote:
The Mycologist said: Some people have had success forcing water into the spores by putting a rubber band on the plunger to increase the pressure inside the syringe.
tried that but didnt seem to work still no germination.
what about heating up the spores in water at a specific temperature for some time?
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