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OfflineCelestialexplorer1
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Re: Uv-c to sterilize agar [Re: Celestialexplorer1]
    #26668077 - 05/13/20 06:45 AM (3 years, 9 months ago)

Quote:

Celestialexplorer1 said:
Obviously I would still pc the agar and use all sterile techniques but just to go a step further you could make a chamber with the light to hit the surface of the agar to kill any possibly lingering bacteria.




This is my original post... did you read it


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To spend just one moment in eternity


Edited by Celestialexplorer1 (05/13/20 06:45 AM)


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InvisibleAndyHinton


Registered: 12/05/16
Posts: 434
Re: Uv-c to sterilize agar [Re: Celestialexplorer1]
    #26668540 - 05/13/20 12:12 PM (3 years, 9 months ago)

Yeah. I just didn't see how it would be different from, "How can I use UV-C to sterilize the space I handle agar in?" Because why would you sterilize something and then sterilize it again in a less effective way? Anyway, I regretted entering the thread after I saw Solipsis's thread.

Some more quick readings. A generic LED bulb in a lamp emitted 400 µW/cm2 of UV. The Carex Day-Light emitted nothing, exactly as advertised.

:peace:


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Edited by AndyHinton (05/13/20 12:24 PM)


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OfflinePinkStormtrooper
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Registered: 04/11/20
Posts: 218
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Re: Uv-c to sterilize agar [Re: AndyHinton]
    #26668851 - 05/13/20 04:10 PM (3 years, 8 months ago)

some light reading about UV-c surface disinfection

http://www.bioline.org.br/pdf?mb08074


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"say, you got a little astroglide on your moustache"


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OfflinePassionateGrower
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Registered: 05/11/20
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Re: Uv-c to sterilize agar [Re: PinkStormtrooper]
    #26685077 - 05/21/20 07:51 AM (3 years, 8 months ago)

FAR-UV...."Seems" to be different and there are several published articles regarding the safety and efficacy. The claims seem high (i.e.) it doesn't penetrate the skin or eyes.

I am no expert on light and just here for the discussion 

The Potential for FAR-UV light


Ushio Far UV 222nm safe for human skin

AndyHinton, what are your thoughts?


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This is my way, what is your way? The way doesn't exist.

`I know how much that means to me`


Edited by PassionateGrower (05/21/20 08:16 AM)


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InvisibleAndyHinton


Registered: 12/05/16
Posts: 434
Re: Uv-c to sterilize agar [Re: PassionateGrower]
    #26688455 - 05/22/20 03:28 PM (3 years, 8 months ago)

Hi PassionateGrower,

I wouldn't let that near me without adequate protection (plastic barrier, amber glasses, and unexposed skin) no matter what the claims.

DNA absorption peaks at 254 nm. I personally use a Qubit instead of a spectrophotometer because it's safer, easier, and more accurate. For fluorescence imaging I only use blue LEDs with an amber filter.

Anyway, for sterilization purposes I'd find a UV-C light with a spectral peak as close to 254 nm as possible. That way it ionizes the DNA as fast as possible, zapping contaminants in minutes. A great way to sterilize a working space for tissue culture.

By the way, I sold the broad spectrum UV radiometer and am expecting a UV-C model soon. So I can measure the specific radius of effect around the tub's outside, if any. Just to appease the bogeymanne in the thread. :smile: The real use, of course, is to certify my bulb myself. Because the efficacy tends to fade after about 7,500 hours.

Also, UV LEDs typically suck. Unless you're prepared to pay big money for the real thing. Practically my whole existence (house, lab, etc.) is outfitted with LEDs. But not my specialized bulbs. For example, my microscope still uses halogen, but when I retrofit it for fluorescence, I'm definitely using a blue LED array.


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Edited by AndyHinton (05/22/20 03:33 PM)


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Invisiblepanne cyanne
albino queen
Female

Registered: 04/15/20
Posts: 145
Re: Uv-c to sterilize agar [Re: AndyHinton]
    #26688717 - 05/22/20 05:16 PM (3 years, 8 months ago)

this thread makes me wanna install the loc-nar in the ceiling of my lab and
control it with a key lock switch outside the room.

victory over contams.
i would see no issue really if people learned to use them remotely.


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InvisibleAndyHinton


Registered: 12/05/16
Posts: 434
Re: Uv-c to sterilize agar [Re: panne cyanne]
    #26707146 - 05/30/20 01:23 PM (3 years, 8 months ago)

Good news! My UV-C radiometer came in. Brand new, factory fresh cert. Calibrated 3 days ago on 2020-05-27.

Any common plastic thicker than cellophane will block most or all UV-C waves. The highest reading I got was 19 µW/cm2 right above the lamp, against the plastic. The intensity decreased by an order of magnitude to 2 µW/cm2 from one foot away. The combination of clear PP5 and amber glasses completely blocked all waves. It was difficult to measure > 1 µW/cm2 from any normal sitting point. But I did reach 7 µW/cm2 around the top perimeter of the tub, right against the plastic.

Some internal readings for comparison. I wrapped my hand in thick cloth, wore long sleeves, donned amber glasses, and took readings inside the irradiated workspace. The intensity around the bottom of the dead air box, where I usually work, ranged from 180–200 µW/cm2 or an order of magnitude higher than any reading outside the tub. Reading the lamp directly maxed out the meter. It only reads up to 2,000 µW/cm2 but I was able to get about 1,950 µW/cm2 a few inches away from the bulb.

The limit when UV-C is considered to be a biohazard: 8 hours of direct continuous exposure to 0.2 μW/cm2 (6 mJ/cm2 total dose). So if I wanted to really damage myself, I would need to expose unprotected mucosa (eyes, mouth, genitals) to the radiation coming off the top of the tub for at least 48 minutes. The damage would basically be a sunburn. I don't even run the bulb for that long because sterilization happens faster than pizza delivery.

The technique works great as long as your bulb is good and you observe some very basic precautions. If done responsibly, it's significantly less dangerous than walking outside on a sunny day. Remember I'm talking about sterilizing a dead air box, not agar. Key takeaways:

  • Only buy non-LED bulbs with manufacturer curves/data sheets available
  • Use the thickest plastic or glass shield and wear amber glasses when it's lighted
  • Assume a non-trivial dose will pass through, but remember it degrades exponentially
  • Look at your setup and rearrange it to minimize any exposure to the bare bulb
  • You'll probably get a bad sunburn if you work under the lighted bulb
  • Even though it's easily blocked, working under UV-C is not a good idea

Anyway, now I can measure my bulb's lifetime and self-certify it. This lets me not use a new bulb in my flow hood every 1,000 hours. One code every 3 years, 7 codes a bulb, 4 bulbs a box. I have infinite free UV-C light and wouldn't have even bothered to find out it if wasn't for Anne Halonium. So thanks for keeping it real.

Quote:

anne halonium said:
the age of the urban uber grower,
with advanced teks is here.
no turning back.




It's possible to build a nice lab bench around UV-C technology instead of, or part of flow hoods. The light is most effective when it's parallel to the the laminar flow. Another reason to consider vertical hoods, besides the disproportionate workspace to filter size ratio. You gotta replace those HEPAs every few years. :thumbup:


Edited by AndyHinton (05/30/20 07:46 PM)


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Invisiblepanne cyanne
albino queen
Female

Registered: 04/15/20
Posts: 145
Re: Uv-c to sterilize agar [Re: AndyHinton]
    #26707764 - 05/30/20 07:07 PM (3 years, 8 months ago)

the future does belong to the growers who can exploit new tech tools best.
UV is not new, but the spirit for innovation in myco grows is strong.


anne halonium was the harley quinn of mycology arts.


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OfflineTripglass420
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Registered: 01/27/20
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Re: Uv-c to sterilize agar [Re: panne cyanne]
    #26749213 - 06/16/20 02:38 PM (3 years, 7 months ago)

Be very careful with uvc as everyone who stated it does damage to eyes I can confirm that through personal experience. I accidentally left one on one night and fell asleep (keep in mind the light was in a closet with the door cracked) and it still emits uv into the air so needless to say I woke up to the worst pain I've ever felt in my life in my eyes and couldn't see at all. My eyes felt like they were filled with sand and I couldn't keep them open for more than one second at a time without tearing up in massive pain. Eye drops actually made the pain ten times worse. Anything I looked at even my phone looked like a star in the sky gleaming so bright I couldn't see a damn thing. I had to lay in bed with a wet rag draped over my eyes for 8hours straight and wasn't back to normal in till the following afternoon. I had only looked at the light for maybe 5minutes total but because it had stayed on for so long it effected the atmosphere of my room just through the door being cracked!

Scariest ducking thing in my life having to wake up thinking I was blind and the pain was a solid 12 on a scale of 1-10. Those things are no joke.
They also seem to be bad for mycelium because I had roughly 5 jars all completely stall after trying to use the light on them.

Personal recommendation is that the light is only good for sterilizing the air in your workspace. Using them on anything directly seemed to have negative effects.

Now as far as sterilizing your equipment of maybe using them on empty syringes/jars during storage does seem to help but all in all I think it was just a waste of money.
Happy shroomin and good luck!


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InvisiblebodhisattaMDiscordReddit
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Re: Uv-c to sterilize agar [Re: Tripglass420]
    #26749448 - 06/16/20 04:08 PM (3 years, 7 months ago)

UV is electromagnetic wave. If you're sleeping and your bulb is in a closet you won't fry your eyes.

UV does go in the air yes but it's like a gas that permeates. If your eyes hurt its because you looked at the bulb at some point earlier before bed. The pain takes hours to develop after the exposure. It wasn't the atmosphere in the room lol.


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OfflineTripglass420
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Re: Uv-c to sterilize agar [Re: bodhisatta]
    #26749823 - 06/16/20 06:50 PM (3 years, 7 months ago)

Well whatever it was it was some serious pain. And your clock must be a little off because the time between looking at the bulb for a few minutes and the pain was less than an hour. Gotta remember that not everyone has the same pain threshold and I'm hypersensitive to eye stuff due to blowing glass for years as well as having blue eyes.

Thanks for the correction though bod you always seem to chime in at just the right time to add in your two cents of passive aggressive loneliness and bahumbuggery haha

And try reading closer next time cause you clearly missed where I mentioned looking at the bulb for about 5min prior to sleeping


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OfflineTripglass420
Old Head back from hiatus
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Registered: 01/27/20
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Re: Uv-c to sterilize agar [Re: Tripglass420]
    #26749825 - 06/16/20 06:51 PM (3 years, 7 months ago)

!maybe I should be careful you don't ban me again for being happier than you

:smile:

Ps. Bodhisatta is actually spelled bodhisattVa but that's just be chiming in to correct you on something that prolly has some sort of explanation to it but I just wanna seem perfect.....wait I forgot that's your job on here haha

Bottom line is in my opinion uvc lights don't do much good. Thats just my opinion though and everyone is entitled to their own. I'm not gonna spread information that could be harmful to someone just to make myself seem knowledgeable to the forums (prolly the only place you get any sort of positive feelings from others )


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Edited by Tripglass420 (06/16/20 06:59 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Uv-c to sterilize agar [Re: Tripglass420]
    #26750068 - 06/16/20 08:23 PM (3 years, 7 months ago)

The spelling of my name depends on the language... It's spelled correctly but not in Sanskrit.

All I'm trying to say was the 5 minutes fucked you. Also the mechanism by which the damage takes place and causes pain is well understood


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OfflineCelestialexplorer1
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Re: Uv-c to sterilize agar [Re: bodhisatta]
    #26819420 - 07/12/20 01:19 PM (3 years, 7 months ago)


For scalpel maybe even agar plates?..


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OfflineCelestialexplorer1
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Registered: 03/25/20
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Re: Uv-c to sterilize agar [Re: Celestialexplorer1]
    #26819435 - 07/12/20 01:29 PM (3 years, 7 months ago)

Again that’s not an idea for complete sterilization but I make my plates about 50 at a time and refrigerate then take out as I need them so one might be able to take two out and run them through that just to ensure it’s sterile. I don’t know probably pointless actually but it made me think of this thread.


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Invisibleretowen
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Registered: 08/05/16
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Loc: Canada
Re: Uv-c to sterilize agar [Re: Celestialexplorer1]
    #26819466 - 07/12/20 01:48 PM (3 years, 7 months ago)

The problem is that many of the uv-c lamps are fake:
https://www.reddit.com/r/PandemicPreps/comments/gqgkyf/beware_of_fake_led_uvc_sanitizers_this_simple/

if you find a real inexpensive uv-c lamp please share!


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