Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Autoflowering Cannabis Seeds

Jump to first unread post Pages: 1
OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
1st Agar Attempt! :) Do things look right ?
    #26747713 - 06/15/20 08:43 PM (3 years, 7 months ago)

So today I started my first attempt at cleaning up my act. Im sure you know what I mean. I have 7 agar plates pressure cooking right now.

After several times dropping them all over my kitchen floor I finally smartened up and placed them on a level surface so that when i placed the lids on they wouldnt fall over. Rookie mistake.

This is what the mixture looked like, i didnt add food coloring because i didnt have any on hand, followed this tek https://www.shroomery.org/forums/showflat.php/Number/23188000 :








I then wrapped them in foil with a paper towel placed underneath to help account for moisture buildup. I forgot to take a picture after that but i then placed them in the pressure cooker on top of the regular metal piece that lifts anything off the bottom of a pressure cooker, as well as some bands from qt jars to keep them from sitting in the water itself (Idk if that matters I just felt better about it).

Waiting now for the pressure cooker to finish up the 35-40 minute run. I will allow them to cool completely inside the pressure cooker and I will be sure to post more as soon as I have more info. TBH though is this worthy of a post in the mushroom cultivation forum or should it just be a journal/ grow log? I apologize if I have placed this in the wrong spot but I was hoping to get opinions on if things look right so far or if someone might think I need to change something. I will move them (once cooled) into the sprayed SAB (with dish soap and water).

The plan is to inoculate them with a single drop of MS syringe and clean up my act as I said earlier. I am sick of having consistently dirty spawn. I need to find a way to better manage all of this. Without you guys I couldnt have even gotten as far as I have with it in the past 5 years.

It blows my mind that I didnt try this sooner. Its relatively easy. If youve done BRF tek, you can do Agar thats for sure. Just about as complicated as BRF tek.


Extras: Filter Print Post Top
OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
Re: 1st Agar Attempt! :) Do things look right ? [Re: EntheoGod]
    #26751519 - 06/17/20 12:15 PM (3 years, 7 months ago)

So I inoculated the agar plates 2 days ago via MS syringe. I am new to this and dont exactly know the time frames im looking at. I have patience so if it takes weeks it takes weeks Id just like to know if on agar they will still take around 4 or 5 days to germinate like they do on WBS. Or should I start over after a few days of no growth on the agar?

Thanks for the help ahead of time like I always say! <3


Edited by EntheoGod (06/17/20 12:21 PM)


Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 14 hours
Re: 1st Agar Attempt! :) Do things look right ? [Re: EntheoGod]
    #26751641 - 06/17/20 01:07 PM (3 years, 7 months ago)

It can take anywhere from 2 to 30 days. If nothing happens in 2 weeks I would start more plates just in case. If they aren't taking up too much space, keep the first set around as well and see what happens.

But honestly, I've never had any that took longer than a week to germ.


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: 1st Agar Attempt! :) Do things look right ? [Re: One of Us]
    #26751759 - 06/17/20 01:59 PM (3 years, 7 months ago)

I've seen germination occur as far as nearly 6 weeks post inoculation... older spores especially can be quite stubborn... try pouring plates with less agar percentage so that theres more moisture present... this, as well as a slight increase in temperature (top shelf of the closet, not incubation) can help

2 days is an unrealistic expectation even with fresh spores... 4 days is about average


Extras: Filter Print Post Top
OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
Re: 1st Agar Attempt! :) Do things look right ? [Re: Caps McGee]
    #26752981 - 06/17/20 10:48 PM (3 years, 7 months ago)

Quote:

Caps McGee said:
I've seen germination occur as far as nearly 6 weeks post inoculation... older spores especially can be quite stubborn... try pouring plates with less agar percentage so that theres more moisture present... this, as well as a slight increase in temperature (top shelf of the closet, not incubation) can help

2 days is an unrealistic expectation even with fresh spores... 4 days is about average





I definitely didnt expect to see anything after a couple days. I was fully expecting about a week or two before anything. Im in no hurry just like knowing if things could be flawed.

I will get a few other things going in the meantime. So I can focus my energy on that. :smile:


Extras: Filter Print Post Top
OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
Re: 1st Agar Attempt! :) Do things look right ? [Re: EntheoGod]
    #26764729 - 06/21/20 05:06 PM (3 years, 7 months ago)

Update: The agar plates are starting to show healthy myc. However I am definitely worried that something will make them unusable considering when trying to drop a single drop of MS spore solution onto the plate I accidentally pretty much put 1/4 of a cc or a bit more in each plate. Every little spot that was hit with the solution is showing healthy myc but is this what I want?



Pics were taken from my iphone and cant seem to get a clearer pic atm.

I know you only wanna do a single drop but would it hurt the results in the end considering the agar plates (if remain healthy) will likely be used to make blenderless LI.

That or I will be introducing the entire agar plate once colonized, into a master grain jar.

This attempt is again, my 1st try with this. And I was using leftover spores from a vendor that isnt a sponsor of the site. I am just happy I didnt mess up making the agar in the first place. 😅

NOTE: Purchased from a site sponsor just a week ago and am very happy with the spores received. Those will be taken better care of.


Extras: Filter Print Post Top
Offlinelateforthafuture
fanatic
 User Gallery

Registered: 03/26/07
Posts: 471
Last seen: 3 years, 4 months
Re: 1st Agar Attempt! :) Do things look right ? [Re: EntheoGod]
    #26765775 - 06/21/20 06:27 PM (3 years, 7 months ago)

Transfer some clean mycelium to a new plate,that way you can see if just clean mycelium grows. There could be something hiding in that germ plate. Don't use the germ plate as inoculant...that kinda defeats the purpose of agar. Even if the germ plate looks clean..transfer as soon as possible.

I've gotten clean cultures on the first transfer from dirty syringes...so do at least one...a few would be better if this is your first time with agar.


--------------------
"Not what could have been, or what should have been, but what will be" -Self
:superbanana::mjdance::dancer:


Extras: Filter Print Post Top
OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
Re: 1st Agar Attempt! :) Do things look right ? [Re: lateforthafuture]
    #26766308 - 06/21/20 07:02 PM (3 years, 7 months ago)

Got it! I appreciate the wisdom. I won’t use these for inoculating anything I’ll use them as a way to practice my agar methods. I’ll make more plates later on with more viable spores but for now if I can get something nice from this then that will be...well...nice lol.

The spore solution I used is very weak in wbs jars.

I’m excited to try transferring and just getting the hang of it all. In your opinion should I transfer some of the mycelium whenever I get the chance or wait a bit longer for it to colonize? Appreciate all the advice!

Also I have read you can make agar into LI easily so I think I’ll still try with that at some point once I get more healthy and clean looking myc. But I’ll likely eventually be able to use agar to knock up some grain jars. The purpose of agar for me is to make sure I have clean inoculant for wbs jars. I’ve just been getting poor syringes up until this point.


Edited by EntheoGod (06/22/20 04:03 PM)


Extras: Filter Print Post Top
Offlinelateforthafuture
fanatic
 User Gallery

Registered: 03/26/07
Posts: 471
Last seen: 3 years, 4 months
Re: 1st Agar Attempt! :) Do things look right ? [Re: EntheoGod]
    #26777653 - 06/23/20 07:44 PM (3 years, 7 months ago)

I would transfer as soon as you see a piece of clean mycelium big enough to grab with a scalpel. Which from the looks of it there's plenty of growth in there...you just have to decide what looks clean and healthy.

There's a good chance the spore solution was "weak" in the jars is because there were contams in there and the mushroom mycelium and contams were duking it out...the mushroom mycelium was probably stuggling.

Once you isolate healthy mycelium and put it into a clean (sterile) grain jar it should show healthy grow. What I'm saying is it's probably not that the spores themselves were "bad" or "weak" or "not viable"...just the fact they were competing with contams made the growth not so strong.


--------------------
"Not what could have been, or what should have been, but what will be" -Self
:superbanana::mjdance::dancer:


Extras: Filter Print Post Top
OfflineForevergreen
Stranger
Registered: 06/23/20
Posts: 173
Last seen: 3 years, 5 months
Re: 1st Agar Attempt! :) Do things look right ? [Re: One of Us]
    #26777685 - 06/23/20 07:58 PM (3 years, 7 months ago)

I poured and inoculated 80 dishes with GT, koh sumui super strain, and Amazon pes. Not only did I not wait long enough for the agar to cool but its been a week with no results but only 3 have contams. Im guessing my vendor has old spores so I'll try a new vendor and 50-100 more dishes in two weeks. Its my first time. Live and learn.


Edited by Forevergreen (06/23/20 07:58 PM)


Extras: Filter Print Post Top
Offlinelateforthafuture
fanatic
 User Gallery

Registered: 03/26/07
Posts: 471
Last seen: 3 years, 4 months
Re: 1st Agar Attempt! :) Do things look right ? [Re: Forevergreen]
    #26777701 - 06/23/20 08:05 PM (3 years, 7 months ago)

Quote:

Forevergreen said:
I poured and inoculated 80 dishes with GT, koh sumui super strain, and Amazon pes. Not only did I not wait long enough for the agar to cool but its been a week with no results but only 3 have contams. Im guessing my vendor has old spores so I'll try a new vendor and 50-100 more dishes in two weeks. Its my first time. Live and learn.




A week is not a long time for spores to germinate...I've waited 30 plus days to see healthy growth.

Next time you inoculate plates with a spore solution try using a sterile swab...squirting a tiny bit of solution onto the swab...then streaking the plate. I've had good sucess with that method and often see healthy growth in 3-4 days (at 70-75 degrees F).

There's a lot of variables at play when germinating spores on agar...


--------------------
"Not what could have been, or what should have been, but what will be" -Self
:superbanana::mjdance::dancer:


Edited by lateforthafuture (06/23/20 08:05 PM)


Extras: Filter Print Post Top
OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
Re: 1st Agar Attempt! :) Do things look right ? [Re: lateforthafuture]
    #26777730 - 06/23/20 08:16 PM (3 years, 7 months ago)

I’m going to scrap the agar I have currently because the myc is super weak and not getting much stronger. It isn’t even doing a good job colonizing any of the 8 wbs jars I made.  Used non sponsor spores for my first agar and the spores were also around 6 months old. I’ll be using healthier spores in next couple of days and I’ll inoculate the agar with the healthy spores. I can tell it’s the spores causing my trouble, considering everything I’ve done with the syringes bought from the non sponsor is literally the weakest mycelium I’ve ever seen. I had some jars fully colonized with the same spores, went to make a tub and it was spitting out mushies before the entire thing colonized, didn’t contaminate at all and even after a soak it did nothing. When I trashed it I could see it hadn’t colonized the substrate fully and wasn’t going to.

I tested this with seeds from the same batch and made a very small tray just to see the speed it colonized, it hasn’t colonized the substrate at ALL over two weeks. Still no contamination’s at all either just no growth on the cvg. It’s absolutely the spores on my end. But I have sponsor spores now :smile:


Extras: Filter Print Post Top
OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
Re: 1st Agar Attempt! :) Do things look right ? [Re: EntheoGod]
    #26779497 - 06/24/20 02:07 PM (3 years, 7 months ago)

The agar I scrapped had a lot of mycelium that was producing metabolites and the growth didn’t seem to be sectoring at all. 😖

It just didn’t look right at all to me. The mycelium itself was super fuzzy as well. Didn’t look like cobweb but I wouldn’t feel safe transferring any of it to new freshly made plates. Instead I’ll be inoculating some freshly prepared agar with golden teacher ms spore solution from a vendor of the site. Hoping these spores will be more viable or have less competing contams. I’ll be cleaning it up on the agar so long as it doesn’t turn out the way those above did. I will make a very detailed write up of everything I did, including pics, as soon as I’m finished inoculating the agar later. Wonder if I should continue to update this post or make a new thread. Feels like something that would be better done in this thread so others can see what happened during the first attempt.  I’ll be sure to show the wbs that I inoculated with the older spores as well in that post because I inoculated a separate batch with one of the new syringes I got that was also golden teacher.  Not seeing growth on those jars yet but it’s a matter of time if you ask me.

Something about the new syringes just looked healthier and better taken care of. The spores weren’t clumping together a ton like the syringe that is in question. 🤔


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Autoflowering Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* 1st time attempt doc34 777 9 02/18/04 06:22 AM
by doc34
* 1st grow attempt... possible hyphal knots? : pics : caspur 6,065 18 06/08/13 06:42 PM
by PussyFart
* 1st casing attempt...straight coco coir or mix? Recipes? Stevo21 1,951 6 06/02/04 06:03 PM
by newbie420
* Re: mold in agar plate Anonymous 12,183 3 12/04/99 12:35 AM
by Anonymous
* Fruit on Agar?? Yarry 5,050 17 01/22/04 09:44 PM
by Anonymous
* golden teacher agar asking fugu 3,052 6 01/31/03 12:59 PM
by debianlinux
* Agar to Rice flour substrates foxxybrown 2,136 3 09/01/03 05:00 AM
by eggy
* Agar Anonymous 1,538 8 04/15/04 10:15 AM
by Bi0TeK

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
342 topic views. 27 members, 178 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.01 seconds on 14 queries.