Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
OfflineBooooooooom
Stranger
Registered: 11/16/15
Posts: 139
Last seen: 2 days, 11 hours
Shook jar --> recovered --> smells weird
    #26745856 - 06/15/20 07:42 AM (3 years, 7 months ago)

Hello,
I have a couple of jars that look ok, they are placed in a Rom Where its 26-27 degres.
This is ofc a bit on the higher side, but I think it's fine. I have unculated using of from agar, had multiple flushes from it meaning it's good.
I have had some jars that's a little bacterial and others that look fine, but when shaken, they do recover fast. But when I smell it, it does not smell like mushrooms, it smells either a bit sweet, or sometimes a bit weird, I can't explain it. Like oats but with something added. I recently tried fruting it, its like a 50% chance it will grow fruits or contam. I'm wondering what this could be,
Is it the hot room that makes the jars bacterial, or is this somehow my LC which I know is fine.


Extras: Filter Print Post Top
InvisibleApples in Mono
Not a puppet


Registered: 09/21/17
Posts: 3,240
Re: Shook jar --> recovered --> smells weird [Re: Booooooooom] * 1
    #26745869 - 06/15/20 07:55 AM (3 years, 7 months ago)

That heat level isn't great, but it won't produce contams on it's own. It'll only provide an environment in which contams will flourish. The contams still have to be introduced.

Either you aren't properly sterilizing your grain jars to begin with, or your LC isn't clean, or your jar lids don't have proper filters , or your inoculation technique is flawed. Or some combination of the 4.

How do you know your LC is clean?


Extras: Filter Print Post Top
OfflineBooooooooom
Stranger
Registered: 11/16/15
Posts: 139
Last seen: 2 days, 11 hours
Re: Shook jar --> recovered --> smells weird [Re: Apples in Mono]
    #26745879 - 06/15/20 08:04 AM (3 years, 7 months ago)

Quote:

Apples in Mono said:
That heat level isn't great, but it won't produce contams on it's own. It'll only provide an environment in which contams will flourish. The contams still have to be introduced.

Either you aren't properly sterilizing your grain jars to begin with, or your LC isn't clean, or your inoculation technique is flawed. Or some combination of the 3.

How do you know your LC is clean?




Well, i know because i took it from a clean agar plate. Then I have used it to produce mushrooms, only know it's started to give me problems.
I sterilize at a pressure cooker for around 100 minutes. I have the oat prep down perfectly.


Extras: Filter Print Post Top
InvisibleApples in Mono
Not a puppet


Registered: 09/21/17
Posts: 3,240
Re: Shook jar --> recovered --> smells weird [Re: Booooooooom] * 1
    #26745906 - 06/15/20 08:18 AM (3 years, 7 months ago)

Even if the agar was clean, the LC could still be dirty. Contams can be introduced when you transfer from the agar to the LC. And even if it was clean to begin with, it could still be dirty now. Every time you use some of it, there's another opportunity to introduce contams.

There's also another chance when you inoculate the jars with the LC.

I suspect that either your LC isn't clean, or your LC to grain inoculation technique is flawed. Or both.

Have you tested the LC on agar recently?

Could you briefly describe your LC to grain inoculation procedure?


Extras: Filter Print Post Top
OfflineBooooooooom
Stranger
Registered: 11/16/15
Posts: 139
Last seen: 2 days, 11 hours
Re: Shook jar --> recovered --> smells weird [Re: Apples in Mono]
    #26745918 - 06/15/20 08:27 AM (3 years, 7 months ago)

Quote:

Apples in Mono said:
Even if the agar was clean, the LC could still be dirty. Contams can be introduced when you transfer from the agar to the LC. And even if it was clean to begin with, it could still be dirty now. Every time you use some of it, there's another opportunity to introduce contams.

There's also another chance when you inoculate the jars with the LC.

I suspect that either your LC isn't clean, or your LC to grain inoculation technique is flawed. Or both.

Have you tested the LC on agar recently?

Could you briefly describe your LC to grain inoculation procedure?




I understand and i also thought about maybe the lc is not clean.
I made my agar with LMEA, then place spore on it 1 drop. After this transfer some growth to new plate, wait and see if it looks ok. Otherwise make new stransfer until clean.
My lc is also made with LMEA, I do everything in a SAB.
I heat the scalpel and make a cut in the plate open up lc jar and place bite there. Then I wait for growth. Around 1 week for growth,

When inoculating jars, same procedure, flame sterilize between injections. Some time I do 2 jars then flame sterilize. I always have gloves and face mask.
My syringe I sterilize using the boiling water, suck and dispose. I have the big needle, without the cap. Lc is stored in the fridge in zip lock.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Reusing Jars mush 2,695 5 11/03/08 10:08 AM
by Breetai
* rye jars innoced in July walt 648 3 10/24/02 07:33 AM
by walt
* no 1/2 pint jars
( 1 2 all )
fugi187 4,667 28 09/28/02 09:39 AM
by StS
* old jars!!! diacamomo 844 5 08/06/02 10:59 AM
by cdlove69
* Re: The smell of contams PhilosoPossum 1,028 1 05/05/00 01:32 PM
by PhilosoPossum
* Why No Quart Jar Colonization? kid_alamo 4,340 14 10/14/02 10:08 AM
by tripndicular
* Jars not doing anything? ble0t 1,019 8 09/15/01 06:28 PM
by ble0t
* Sour Apple Like Smell MilhouseT1 1,403 1 03/02/02 07:26 PM
by bluhoney

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
167 topic views. 26 members, 210 guests and 57 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.009 seconds on 14 queries.