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OfflineCatsLoveHouseMusic
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Should I make LC if I plan on inoculating a lot of grain jars?
    #26744614 - 06/14/20 06:12 PM (3 years, 7 months ago)

So I started usijg syringes, which worked really well. I now use agar, butI fudged up my first 5 jars somehow. Its been like 4 days since dropping the agar in the jars, they look great and I dont see any contams but well see. I was thinking making LC would be a goid move but Im not sure. Thoughts?


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OfflineCocaineBuffet
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: CatsLoveHouseMusic]
    #26744627 - 06/14/20 06:20 PM (3 years, 7 months ago)

Sure just test on agar before grain to make sure it is clean.


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OfflineAtmozFear
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: CatsLoveHouseMusic]
    #26744635 - 06/14/20 06:26 PM (3 years, 7 months ago)

from what I hear, and have experienced with LI (liquid inoculate, only difference is level of liquidation) is that contamination is harder to spot than it is on the agar plate, due to being 3D instead of 2D (agar)


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OfflineItsaMeShroomio
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: CocaineBuffet]
    #26744638 - 06/14/20 06:27 PM (3 years, 7 months ago)

You're probably better off doing A2G then G2G, rather than LC.

It's very hard to tell if/how-badly contaminated an LC is, compared to agar or grains.


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OfflineAtmozFear
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: ItsaMeShroomio]
    #26744640 - 06/14/20 06:29 PM (3 years, 7 months ago)

:whathesaid:


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OfflineCatsLoveHouseMusic
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: AtmozFear]
    #26744687 - 06/14/20 06:52 PM (3 years, 7 months ago)

Oh understood. I wqs hoping I could skip the part where I open the jar lid. I do it in a closed door closet, in SAB, alcohol/torch everything with gloves/mask, with 4th transfer agar and just get nervous.

Ill keep the best looking jar as a master jar, right? You do it the same as agar id assume, ill read on it more.


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OfflineAtmozFear
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: CatsLoveHouseMusic]
    #26744696 - 06/14/20 06:55 PM (3 years, 7 months ago)

yeah but if there is a contamination in the LC, I think it amplifies a lot faster then on an agar plate


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OfflineItsaMeShroomio
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: CocaineBuffet]
    #26744704 - 06/14/20 07:00 PM (3 years, 7 months ago)

I always hear this about testing LC on agar first, but IME the LCs I have tested on agar all look clean for around 10-14 days or so, then comes some yellow metabolites if it wasn't actually clean.

I'm too impatient to do anything but inoculate and test in parallel, so the test never really bought me much.


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OfflineGan
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: AtmozFear]
    #26744714 - 06/14/20 07:08 PM (3 years, 7 months ago)

Quote:

AtmozFear said:
from what I hear, and have experienced with LI (liquid inoculate, only difference is level of liquidation)




The difference is LC contains nutrients to allow the culture to grow in the liquid medium. LI is just myc in sterile water.

Yes OP always test your LC's. And if you're doing a large number of jars, g2g, LI, or LC is going to be the way to go. Assuming you have clean spawn, g2g is probably going to be the easiest


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InvisibleApples in Mono
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: CatsLoveHouseMusic]
    #26744754 - 06/14/20 07:20 PM (3 years, 7 months ago)

I did LC once to try it out. Haven't felt the urge to do it again. I just do LI. I see no good reason for LC, at least not for any of the species I've grown or the amounts I'm growing. An LI can even be stored in the refrigerator for extended periods. Not sure how long, but I've heard reports of over a year with no problems. Fuck LC- LI FTW


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OfflineAtmozFear
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: Gan]
    #26744810 - 06/14/20 07:45 PM (3 years, 7 months ago)

Quote:

Gan said:
Quote:

AtmozFear said:
from what I hear, and have experienced with LI (liquid inoculate, only difference is level of liquidation)




The difference is LC contains nutrients to allow the culture to grow in the liquid medium. LI is just myc in sterile water.

Yes OP always test your LC's. And if you're doing a large number of jars, g2g, LI, or LC is going to be the way to go. Assuming you have clean spawn, g2g is probably going to be the easiest




well what do you think it is that you transfer the mycellium from?... you drop a bunch of pieces of colonized agar, which is very nutritious obviously.

LI is just more fluid because yes, more of it is water, and because you use a blender to further liquify it.


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Edited by AtmozFear (06/14/20 07:49 PM)


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OfflineGan
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: AtmozFear]
    #26744833 - 06/14/20 07:58 PM (3 years, 7 months ago)

Quote:

AtmozFear said:
well what do you think it is that you transfer the mycellium from?... you drop a bunch of pieces of colonized agar, which is very nutritious obviously.




I was literally just explaining the difference between LI and LC lol. The difference is that LI is made from sterile water with no additives. LC is made with water plus nutritious additives such as BRF, grain water, LME, etc.

Sure, in a pedantic sense, you are technically including a nutrition source in your LI via the agar the myc is housed on lol. However, the liquid medium the culture is suspended in does not contain any nutritional additives initially, unlike LC.

LC allows one to expand a culture an absurd amount, which is not possible with LI. A piece of myc, via the biopsy method, smaller than is visible to the naked eye can expand to 2L+ of LC if one truly desired that much. With LI, the amount of myc in the liquid is more or less only as much as you decide to add from the transfer. The culture will expand very little once transferred to LI.

OP, if you practice good sterile technique LI and/or LC are both great ways to inoculate large amounts of grain. Everyone has their preferences for one over the other. And there are certain scenarios that are better suited for LC, while other situations call for LI. Play around with both if you have time. It's a great skill to learn.


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Edited by Gan (06/14/20 08:28 PM)


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OfflineAtmozFear
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: Gan]
    #26744885 - 06/14/20 08:23 PM (3 years, 7 months ago)

ok actually learned something then.

and i also learned the word "pedantic" lol


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Edited by AtmozFear (06/14/20 08:26 PM)


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OfflineGan
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: AtmozFear]
    #26744902 - 06/14/20 08:33 PM (3 years, 7 months ago)

Hope you didn't take offence. I wasn't using pedantic in the smart ass/sarcastic way. I was using it as emphasizing a technically accurate, yet very minor, detail. Cheers man. I've tried LC a few times. I like it, but I honestly don't grow enough that I'm able to use it to its fullest capacity. I really like LI as well.

If you're interested in LC a bit now, go look up Josex's biopsy tek to see how much LC can expand a culture. It's kind of crazy but it's a great technique to know exists in case there ever comes a time when you need it.


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OfflineA.k.aM
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: Gan]
    #26744980 - 06/14/20 09:17 PM (3 years, 7 months ago)

Lc is great when it works.

I’d probably wait until you’ve knocked up more jars and done more sterile work though.

But really if you can pour plates and make transfers without getting contams then you can do lc. I’ve been making them in media bottles like agar and then pouring them into jars. It’s exactly like pouring plates so if you can do that cleanly you’re good.

Same goes for making the lc. If you can transfer a wedge to a plate you can do it to the media bottle.

I used needles the first couple times but really it’s just another way to contaminate it.


Making super soft plates that liquify when you shake the jar is another easy way.


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LAGM2020


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OfflineCatsLoveHouseMusic
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: A.k.a]
    #26744996 - 06/14/20 09:25 PM (3 years, 7 months ago)

I have fourth transfer agar that comes out clean every transfer, so Im confident with it. I personally just dont like opening the tops of the jars, maby its just bc I messed up my first agar to grain. I was cocky because everything Ive done so far turns out amazing, so I just did it quickly.

This last time I took my time, actually cleaned the closet its in, etc... so I figure itll work. I mean LC has s essentially just liquid agar huh? Jusy like shooting agar into the jars. I mean, if I shoot some of the LC onto agar, and it goes well, my LC SHOULD be fine right? Untill the LC is finished, I will keep dropping agar, I have a ton of it.


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OfflineAtmozFear
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: Gan]
    #26745671 - 06/15/20 05:50 AM (3 years, 7 months ago)

Quote:

Gan said:
Hope you didn't take offence. I wasn't using pedantic in the smart ass/sarcastic way. I was using it as emphasizing a technically accurate, yet very minor, detail. Cheers man. I've tried LC a few times. I like it, but I honestly don't grow enough that I'm able to use it to its fullest capacity. I really like LI as well.

If you're interested in LC a bit now, go look up Josex's biopsy tek to see how much LC can expand a culture. It's kind of crazy but it's a great technique to know exists in case there ever comes a time when you need it.




not at all-  I try to be a sponge for learning!  I've noc'd up many many jars with blender-LI from a syringe... never tried LC.


Edited by AtmozFear (06/15/20 05:52 AM)


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OfflineB21
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: AtmozFear]
    #26745708 - 06/15/20 06:16 AM (3 years, 7 months ago)

Imo grain to grain transfers are a fast and effective way to get more spawn. I love lc/li but always test it first! My preferred method; ms/clone to agar, make a few transfers, inoculate, g2g, g2g, spawn.
Good luck! You got this!


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OfflineAtmozFear
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: B21]
    #26745745 - 06/15/20 06:33 AM (3 years, 7 months ago)

yeah the reason I was using LI syringes was so I could just inject thru a SHIP without opening the jar... at the time, my SAB technique sucked.  I recently modified my SAB and put shelves inside which helped, hinged the doors with plastic ties and added a mini table and door knobs.  I don't want to hijack the thread tho, maybe I'll take some pics and do a post.


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OfflineCatsLoveHouseMusic
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Re: Should I make LC if I plan on inoculating a lot of grain jars? [Re: AtmozFear]
    #26746468 - 06/15/20 12:22 PM (3 years, 7 months ago)

Please post pictures. Im trying to learn.


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