Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Clone on agar
    #26744870 - 06/14/20 08:17 PM (3 years, 7 months ago)

How many transfers would a clone agar plate need to be clean enough for grain.an how many transfers for a single sector isolate from a clone


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 13 minutes
Trusted Cultivator
Re: Clone on agar [Re: Mycobro420]
    #26745004 - 06/14/20 09:30 PM (3 years, 7 months ago)

Clean is clean there’s no number of transfers. If you do the tissue sample well then zero transfers.

Isolates can take like 50+ transfers and seem to suck more often then not.

Usually with clones a couple transfers will be enough to get consistent results.


--------------------
LAGM2020


Edited by A.k.a (06/14/20 09:30 PM)


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: A.k.a]
    #26745791 - 06/15/20 06:58 AM (3 years, 7 months ago)

Ya I'm just worried about white mold an always thinks my cultures have them I ust to do fruit tissue peice to mea wraped in parafilm then sector the edges of dish to grain to new plate but I got trick on a few of them.so know I'm trying 2 transfers from master dish an see how that works I'm just confused what to look for cause I get rizomorphic growth from every dish including the flesh dish.also I have a flowhood


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: Mycobro420]
    #26745797 - 06/15/20 07:00 AM (3 years, 7 months ago)

How many transfers would you recommend I personally don't see much wrong with my petris no bateria spots no discoloration just white circular rizo growth.every now an then it wount be 100% circular Anni usually toss them


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
Trusted Cultivator
Re: Clone on agar [Re: Mycobro420]
    #26745853 - 06/15/20 07:40 AM (3 years, 7 months ago)

Can't answer that. A plate is clean when it's clean. It could take as a little as one transfer until it's clean.

You just want to make sure when you transfer that you take healthy tissue that is far away (and not on top of) bacteria and/or mold colonies.
Bacteria can sometimes present as a thin film that can cover most of the top of the plate so watch out for that.
Don't take tissue that isn't from the culture - white spots in random places on the plate could be one of two things, mycelium that fell off the wedge (maybe the wedge rolled a little), or they are mold colonies. It can be hard to distinguish between the two so I just don't transfer from them.

Don't get hung up on rhizomorphic and tomentose growth. A heavily tomentose (fluffy) culture will often turn rhizomorphic when transfered to media with less nutrients, and vice versa.

Just look for clean, healthy looking growth that's far from contams to transfer.


--------------------

Amateur Mycologists United
AMU Q&A


Edited by mushpunx (06/15/20 07:53 AM)


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
Trusted Cultivator
Re: Clone on agar [Re: mushpunx]
    #26745864 - 06/15/20 07:50 AM (3 years, 7 months ago)

Also dude you need to use more punctuation though, your posts are really hard to read. :lol:

Again nobody can tell you how many transfers it will take to reach an isolate.

I don't think you understand what a sector is from the way you are using the term.
Early transfers of a culture from spore or clone often have hundreds of strains present, so they will often look uniform and round. The more transfers you take though, there will be less and less strains present and you can start to see the lines of separation between individual sectors - there are simply too many strains present to see sectoring until a number of transfers have been made and many of the strains have been cut out.

When you have an isolated strain, it will be a monoculture - perfectly round with no visible sectors.

Clones will often have less strains present than multi spore cultures.
Every once in a while you will take a clone and it will already be a monoculture (no sectoring). Sometimes you will be able to see sectoring right away and other times not.
It usually takes some transfers to see obvious sectoring, but less transfers than an MS culture would take.
I usually just keep clone cultures as is. Sometimes I will transfer them down to a monoculture. The most important thing is to label and test your clones , including potency.

With MS cultures it can often take 8 - 10 or more transfers until you see obvious sectoring. Then you want to take a transfer from each sector - the goal is to end up with many isolates from a culture at first, not one. Sometimes the transfers will continue to sector and you will need to continue to make transfers.
Aim to end up with at least 10 isolated strains.
You then label and store each isolate and fruit out each one and test them to compare. The best fruiting and most potent isolate is saved and put to slant.


Here is a really great thread about cleaning and strain isolation on agar, with great photos :

https://www.shroomery.org/forums/showflat.php/Number/18430998


--------------------

Amateur Mycologists United
AMU Q&A


Edited by mushpunx (06/15/20 08:02 AM)


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: mushpunx]
    #26746154 - 06/15/20 10:18 AM (3 years, 7 months ago)

Ok my bad I know my punctuation is sloppy bear with me lol.i understand that there are hundreds of strains on a single Petri sectoring is the fuzzy looking stuff in-between the rizomorphic growth. I'm not looking for a monoculture just good fruiting mycilum witch I know is more or trial and error. But really I'm trying to figure out if my agar is clean and free of hidden white mold.ill upload a pic as well mabey that can say more than I can.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 13 minutes
Trusted Cultivator
Re: Clone on agar [Re: Mycobro420]
    #26746167 - 06/15/20 10:28 AM (3 years, 7 months ago)

Sectors are just different areas of growth, on a plate they’re like pizza slices.  They can be rhizo or tomentose. There’s some good threads on it where guys outline sectoring on their plates.

This plate has some distinct ones.


Just below 9 to about 10 is a sector, and then there’s a super clear one with fuzzier myc from about 11-12

I did a quick rough edit to outline some of the sectors

Even within those there are multiple smaller ones


Edited by A.k.a (06/15/20 10:37 AM)


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: A.k.a]
    #26746292 - 06/15/20 11:11 AM (3 years, 7 months ago)

Ok having trouble uploading my picture let's see if this workshttps://www.shroomery.org/forums/files/20-25/224075427-IMG_20200615_120639400.jpg


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: Mycobro420]
    #26746307 - 06/15/20 11:14 AM (3 years, 7 months ago)

Ok I suck at this I don't know how to up load them


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: A.k.a]
    #26746328 - 06/15/20 11:22 AM (3 years, 7 months ago)

With those pics I see how it's sectoring but another question how can u tell tomentose growth from white mold


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
Trusted Cultivator
Re: Clone on agar [Re: Mycobro420]
    #26746342 - 06/15/20 11:26 AM (3 years, 7 months ago)

Quote:

Mycobro420 said:
Ok I suck at this I don't know how to up load them




Go to "pics" and click upload


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 13 minutes
Trusted Cultivator
Re: Clone on agar [Re: Mycobro420]
    #26746348 - 06/15/20 11:28 AM (3 years, 7 months ago)

Usually it looks slightly different than myc but it can be really hard to do.

Luckily I barely ever see mold that looks like myc. That I know of :uhoh:

Most of the mold I get is dark or yellow, and if it is white it’s got long scraggly hairs.


--------------------
LAGM2020


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
Trusted Cultivator
Re: Clone on agar [Re: Mycobro420]
    #26746364 - 06/15/20 11:35 AM (3 years, 7 months ago)

Quote:

Mycobro420 said:
With those pics I see how it's sectoring but another question how can u tell tomentose growth from white mold




When germinating spores we swipe them across the plate in a zig zag motion, back and forth. You should be able to see germination in that pattern and only taking tissue from that will increase your chances of taking clean myc.

When it is a clone or a transfer, be careful to take tissue that is expanding out from the wedge. Never colonies.


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery

Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
Trusted Cultivator
Re: Clone on agar [Re: Mycobro420]
    #26746371 - 06/15/20 11:37 AM (3 years, 7 months ago)

Quote:

Mycobro420 said:
Ok my bad I know my punctuation is sloppy bear with me lol.i understand that there are hundreds of strains on a single Petri sectoring is the fuzzy looking stuff in-between the rizomorphic growth. I'm not looking for a monoculture just good fruiting mycilum witch I know is more or trial and error. But really I'm trying to figure out if my agar is clean and free of hidden white mold.ill upload a pic as well mabey that can say more than I can.





If all you are looking for is good fruiting myc then simple. Just keep taking clones and label them, then test them until you find a good one.

You should be able to get canopy flushes from MS grows. You need to keep improving sterile tek and how you dial in your tubs.


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: mushpunx]
    #26746502 - 06/15/20 12:41 PM (3 years, 7 months ago)

I can get canopy flushes from Ms around 112g dry. I just ran my first bigger agar cycle to monotubs. I yeilded my best was 158g dry so it was a canopy sort to speak just looking for improvement cause I know 200g dry is possible. But why I made this thread is cause some of my bins caught trick on there first flush witch I know came from the agar or steril Tek or my g2g but first I wanted to figure out if the agar was the problem


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: Mycobro420]
    #26746507 - 06/15/20 12:43 PM (3 years, 7 months ago)

[embed=,]https://www.shroomery.org/forums/files/20-25/224075427-IMG_20200615_120639400.jpg[/embed]


Extras: Filter Print Post Top
OfflineMycobro420
Stranger
 User Gallery

Registered: 06/01/20
Posts: 346
Last seen: 1 year, 7 days
Re: Clone on agar [Re: Mycobro420]
    #26746529 - 06/15/20 12:53 PM (3 years, 7 months ago)

I still can't find how to post pic I got it posted on my account gallery but everytime I try to share it come up like the one before


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* agar transfers buggers 4,514 12 11/03/07 10:44 AM
by orchidfanatic
* agar transfer substitution? omniscientlore 1,478 3 12/19/22 07:14 AM
by Screwup
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 52,762 43 07/08/23 07:04 AM
by Screwup
* G2G transfers...an endless cycle? ADoobie 7,187 18 11/12/20 07:10 PM
by FredsGotSlacks
* agar to agar transfer ohmatic 15,620 11 01/12/21 09:00 PM
by bodhisatta
* How to make agar 2 agar transfer? esp 6,652 7 01/08/04 11:08 AM
by YidakiMan
* agar transfers *DELETED* thisone 1,245 3 11/20/03 08:58 PM
by Mycomancer
* A couple ?'s re: clones, agar, & casings keyeghost 985 2 02/27/04 12:12 PM
by keyeghost

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
989 topic views. 16 members, 168 guests and 35 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.004 seconds on 12 queries.