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OfflineBaconman

Registered: 06/14/20
Posts: 14
Last seen: 2 years, 9 months
Green mold on pink oyster
    #26743511 - 06/14/20 07:16 AM (3 years, 7 months ago)

Hi there,

last time I was using a message board like this is quite some years ago, but im really glad to be back, since I found my new hobby: mushroom cultivation :smile:

I only had two more or less successful mushroom harvests yet.. one was magic mushrooms and the other was Shiitake (which didn't provide too much yield..). So Im quite a nooby.

I got advised to try pink oyster as next, since my room temperature is kinda high in summer (25°C+, 77°F+). The whole growing phase of the mycelium inside the plastic bags worked pretty well. I used saw dust mixed with wood chips and soaked wheat grains as a substrate. Once I saw some of the mycelium turn pinkish I cut off the upper part of the bags and placed them in a humidity tent which was at a more or less constant humidity level of about 85-95%. Pinning started very rapidly on the top of the substrate, but I had the feeling that it also stopped quickly in some areas to proceed in others. There were a lot of very small mushrooms that didn't really proceed growing. You can still see a lot of them on the attached pictures.

Some of the mushrooms where also turning white at some point, which didn't make them look very healthy. Any ideas why that was happening?

A couple days later I was able to spot some mold on some of the grain spawn I used for inoculation, which way laying on the top after mixing. I removed it, knowing that it probably already spread. again a couple days later I spotted green mold on all my 4 substrate blocks, mostly on the mushrooms that turned white (are they dead?) but also to some degree on pink ones. Sadly I ended up throwing all the blocks away and just harvest a few immature ones for cloning. One of the below images is showing one of the immature mushrooms.



Can anyone tell me what I did wrong? Or any tips for how to prevent this?

I saw a lot of people cutting holes in the sides of their backs. Would that have concentrated the pinning?

Thanks a lot in advance and greetings from Europe!


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OfflineSHROOMSISAY01M
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Registered: 01/22/17
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Loc: Virginia, USA
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Re: Green mold on pink oyster [Re: Baconman]
    #26743818 - 06/14/20 10:31 AM (3 years, 7 months ago)

That block would have produced well if you would have Pressed all the air out of the bag and made one slice on the bottom of the bag from corner to corner and flipped the bag upside down. The reason you got all that funny growth is no airflow. Oysters need FAE or you end up with that. I would assume the mold is the same problem no airflow witch causes too much moisture and is a perfect condition for mold.

On your next grow get more FAE you can not have too much FAE with oysters. If you can keep the humidity up there is no way to have too much airflow. 3 to 6 full air exchanges an hour is a good place to be. It is a good idea to have a circulation fan in your grow chamber but don't have it blowing on the mushrooms. The circulation fan is meant to move air around and reduce pockets of co2.

The reason I cut the bag on the bottom is that the bottom is the place where the sub is tightest against the bag. When you slice the bag you want to avoid lifting the bag. Only the tiny slice should be exposed to air. If you lift the bag you will be ok but you will get fruits inside the bag which is best to avoid.

I hope this helps you out!!


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OfflineSHROOMSISAY01M
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Registered: 01/22/17
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Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26744453 - 06/14/20 04:45 PM (3 years, 7 months ago)

In my defense after answering this one. The life-saving crew was called for me my blood sugar 21 but the good news is I survived!! I don't remember answering this. I lmao after reading this.


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OfflineForresterM
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Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26745550 - 06/15/20 04:36 AM (3 years, 7 months ago)

Pinks don't really grow nearly as big as other oysters, generally.  I think what happened there is by exposing the whole substrate, you got WAY too many pins like you said, and they only grew very small.

The reason they grew mold is because they should have been harvested probably a while ago.  Understandable that you didn't, since they were so small they barely look like mushrooms.

Do like shrooms said and don't let the whole surface pin next time, get less pins and the mushrooms will grow bigger :thumbup:


Quote:

SHROOMSISAY01 said:
In my defense after answering this one. The life-saving crew was called for me my blood sugar 21 but the good news is I survived!! I don't remember answering this. I lmao after reading this.




Holy crap that's low.  My daughter is diabetic and I think the lowest I've ever seen is like 40something, eesh!  Glad you're alright!


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineSHROOMSISAY01M
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Re: Green mold on pink oyster [Re: Forrester]
    #26746374 - 06/15/20 11:38 AM (3 years, 7 months ago)

I have a bag right now that has been ready to fruit for a while, but I have done nothing with it. It has the same looking stuff in it. This leads me to believe that it is more of a fresh air thing but either way slice the bag and get plenty of fresh air and you have solved your problem.

Forrester, make sure you get your daughter into good habits with taking care of her diabetes. I had four friends that were diabetic that did not do the right things with there diabetes and all of them have been dead for years now. She can have a long healthy life if she does what's right. I have been diabetic for 40 years with basically no health issues from it. The lowest my sugar has been is 19.


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OfflineForresterM
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Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26746809 - 06/15/20 03:11 PM (3 years, 7 months ago)

Yeah she's very healthy luckily!

I'm sure you're right about the FAE as well, I'm sure it played a part there.  You can see how overmature they are tho


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineBaconman

Registered: 06/14/20
Posts: 14
Last seen: 2 years, 9 months
Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26746987 - 06/15/20 04:19 PM (3 years, 7 months ago)

Hi, and thank you for the responses :smile:

Quote:

SHROOMSISAY01 said:
In my defense after answering this one. The life-saving crew was called for me my blood sugar 21 but the good news is I survived!! I don't remember answering this. I lmao after reading this.




That sounds horrible! Im glade you made it! Sometimes you forget that there are real people on the other side.

Thanks for your advice! I hope the cloning worked without contamination (I still have trouble preventing the agar sterilization from being a complete mess lol) so I can try a new grow quite soon.

Is there something like a best practice way for keeping FAE high, while also keeping humidity high? Do I need a special humidifier with tubing or can I just use a fan to constantly blow some air into the tent?


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OfflineSHROOMSISAY01M
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Registered: 01/22/17
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Re: Green mold on pink oyster [Re: Baconman]
    #26747390 - 06/15/20 07:21 PM (3 years, 7 months ago)

I exhaust air out of the room and do not blow air into it and I have a dampener that shuts when the fan is not on and opens when it is on. My room is tightly sealed so with the fan on it creates a suction and opens the dampener and air is pulled in through the dampener from another room. I built a high pressure mist system from a pressure washer so I have no problem keeping the humidity up. I am not sure why but I did not have my pressure washer come on any more often running my exhaust fan 24/7 than when I was changing the air 4 times an hour. If it did change it was slight enough that I did not notice.


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OfflineLu-Kthu
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Registered: 06/03/20
Posts: 13
Last seen: 1 year, 9 days
Re: Green mold on pink oyster [Re: Baconman]
    #26747916 - 06/15/20 10:55 PM (3 years, 7 months ago)

You growing them inside or out?  I used to grow pinks quite a bit, but kinda stopped.  With your temps I'd just say toss them outside in the shade, direct light will bleach the pink color to almost white.


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OfflineSHROOMSISAY01M
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Re: Green mold on pink oyster [Re: Baconman]
    #26748965 - 06/16/20 01:15 PM (3 years, 7 months ago)

Quote:

Baconman said:
Hi, and thank you for the responses :smile:

Quote:

SHROOMSISAY01 said:
In my defense after answering this one. The life-saving crew was called for me my blood sugar 21 but the good news is I survived!! I don't remember answering this. I lmao after reading this.




That sounds horrible! Im glade you made it! Sometimes you forget that there are real people on the other side.

Thanks for your advice! I hope the cloning worked without contamination (I still have trouble preventing the agar sterilization from being a complete mess lol) so I can try a new grow quite soon.

Is there something like a best practice way for keeping FAE high, while also keeping humidity high? Do I need a special humidifier with tubing or can I just use a fan to constantly blow some air into the tent?




I am not sure why your agar is a mess, but I use Polypropylene pitcher for agar it has a handle so no more holding the bottle with a rag. I cover with aluminum foil everything but the spout and then take another piece of aluminum foil and go over the whole top. So all I have to do is take the top piece of foil off to pour.

https://www.amazon.com/1000ml-Polypropylene-Beaker-Handle-graduations/dp/B00UZCICJE/ref=sr_1_7?crid=3AMO3N64VMNL3&dchild=1&keywords=1000ml+beaker+polypropylene&qid=1592334636&sprefix=1000ml+Polypropylene+beaker%2Caps%2C180&sr=8-7


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OfflineCujllickduo
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Registered: 06/13/15
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Loc: England Flag
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Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26750807 - 06/17/20 06:25 AM (3 years, 7 months ago)

They're delicious


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OfflineBaconman

Registered: 06/14/20
Posts: 14
Last seen: 2 years, 9 months
Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26752060 - 06/17/20 04:07 PM (3 years, 7 months ago)

Quote:

Lu-Kthu said:
You growing them inside or out?  I used to grow pinks quite a bit, but kinda stopped.  With your temps I'd just say toss them outside in the shade, direct light will bleach the pink color to almost white.




I grow them inside and don't have a garden :smile:

Quote:

SHROOMSISAY01 said:

I am not sure why your agar is a mess, but I use Polypropylene pitcher for agar it has a handle so no more holding the bottle with a rag. I cover with aluminum foil everything but the spout and then take another piece of aluminum foil and go over the whole top. So all I have to do is take the top piece of foil off to pour.

https://www.amazon.com/1000ml-Polypropylene-Beaker-Handle-graduations/dp/B00UZCICJE/ref=sr_1_7?crid=3AMO3N64VMNL3&dchild=1&keywords=1000ml+beaker+polypropylene&qid=1592334636&sprefix=1000ml+Polypropylene+beaker%2Caps%2C180&sr=8-7




Its not so much pouring the agar into the petri dish. I think it simply boils over during sterilization.. When I take the petri dishes out of the pressure cooker they are completely covered with water which seems to be mixed with a little bit of agar. All of my 5 agar plates ended up having contamination. Some showed bacteria contamination at the edges, another had sprinkles of mold everywhere and yet another one had only green mold where I touched the agar to place the spores.

I have only spores left now. So I will order new pink oyster spawn soon and try a new batch of agar plates this weekend. Wish me luck lol


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OfflineSHROOMSISAY01M
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Registered: 01/22/17
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Loc: Virginia, USA
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Re: Green mold on pink oyster [Re: Baconman]
    #26752086 - 06/17/20 04:22 PM (3 years, 7 months ago)

What method are you using for Petri dishes? To sterilize the agar and pour it in sterile dishes is the best but you may not have a flow hood or still air box. What method are you using for Petri dishes?


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OfflineBaconman

Registered: 06/14/20
Posts: 14
Last seen: 2 years, 9 months
Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26753933 - 06/18/20 10:22 AM (3 years, 7 months ago)

I create the agar in a pot (I bring water, agar and malt extract to a boil, thats it) and pour it then into Petri dishes. I then stack up to 5 Petri dishes above each other and wrap them in aluminium foil. Afterwards I pressure cook the created stacks of Petri dishes for 30 min in my pressure cooker. Thats my method.

I actually tried to build me a DIY flow hood from an air filter and a cardboard box which I sealed with hot glue. I think its not working very well though. I will probably move to a still air box. The funny thing is that I had no problems at all, when I was cloning my shiitake. I didn't even had a flow hood back then, I was simply doing it on my desk.


Edited by Baconman (06/18/20 10:23 AM)


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OfflineSHROOMSISAY01M
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Re: Green mold on pink oyster [Re: Baconman]
    #26754320 - 06/18/20 01:15 PM (3 years, 7 months ago)

Look up Pasty Plate that is probably your best option.

Here is a link...

https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1

I have never used it but I hear good things.


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OfflineBaconman

Registered: 06/14/20
Posts: 14
Last seen: 2 years, 9 months
Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26754535 - 06/18/20 02:40 PM (3 years, 7 months ago)

Thank for the link.

Maybe a little bit stupid question, but whats the main difference to my method except that pasty plate uses plastic containers instead of Petri dishes?

What is the major benefit? :smile:


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OfflineSHROOMSISAY01M
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Re: Green mold on pink oyster [Re: Baconman]
    #26754683 - 06/18/20 03:15 PM (3 years, 7 months ago)

The main difference is the container. The container is taller. So the agar will not boil over and wick in contamination. With standard Petri dishes, the agar is sterilized and poured into sterilized dishes in front of a flow hood. Without a flow hood, you need to pour into the dishes and sterilize the dish and the agar at the same time. If the agar boils over (which it has to). The Petri dishes is just wicking in contamination.


Edited by SHROOMSISAY01 (06/18/20 04:03 PM)


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OfflineBaconman

Registered: 06/14/20
Posts: 14
Last seen: 2 years, 9 months
Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26754737 - 06/18/20 03:31 PM (3 years, 7 months ago)

Right, that makes sense! Thanks. I will try to find some similar containers and to give it a try.


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OfflineSHROOMSISAY01M
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Re: Green mold on pink oyster [Re: Baconman]
    #26754817 - 06/18/20 04:02 PM (3 years, 7 months ago)

Good luck my friend!!


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OfflineSHROOMSISAY01M
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Re: Green mold on pink oyster [Re: SHROOMSISAY01]
    #26754913 - 06/18/20 04:39 PM (3 years, 7 months ago)

You could also use 4 oz mason jars I think they make them shallow like you would want them to be.


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