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B_happy28
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Noob 99% humidity in bulk substrate monotub
#26724191 - 06/06/20 01:45 PM (3 years, 8 months ago) |
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Hello, noob here. First time doing this and we are Following the monotub instructions by Portland Psych group. We just put two fully spawned rye berry grain quart jars and lasagna them into a 56 qt tub and put a garbage bag over it to wait until it fully colonizes the substrate. We used the pillow case method of pasteurization of verm and coco plus a little bit of black kow. I should have just done the Verm and coco but I know the species I’m growing likes poop. It was the only poop I can find. We hung the pillow case for probably 16 hours but it seem very moist. It didn’t drip when not squeezed but it did produce water when squeezed hard.
My question is, it’s been at 74 degrees for 2 days but 99% humidity. Is that too high?? There is condensation on the tub.
Also, our quarts were perfectly colonized no contams but I had to wait for coco to come in the mail and when we birthed them I did see one small spot from deep inside the jar with very light greenish tinge. I carefully scooped that small bit out and chucked it and changed gloves. I decided to not use heat because of that. I want the mycelium to be stronger and I’m afraid if I bump heat to 80 that could give contam the footing to take over.
Here is the jar before we put it to bulk substrate.
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: Noob 99% humidity in bulk substrate monotub [Re: B_happy28]
#26724195 - 06/06/20 01:50 PM (3 years, 8 months ago) |
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Patiences my friend. Also the tub doesn’t need to be covered with a bag. Mushrooms benefit from light. 2 days is no time to start stressing. Leave it alone for at least 7-10 days then start with your pinning triggers.
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Chuck Shroomer
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Re: Noob 99% humidity in bulk substrate monotub [Re: Strainsfordaze]
#26724341 - 06/06/20 03:00 PM (3 years, 8 months ago) |
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If you have luck your grow will be fine but it is better to not mix contaminated jars with clean ones because all could be ruined if a bad contam has left behind spores or growth you could not see. Manure is also not required for most species and it gives beginning growers more chance of failure.
Waiting and watching for contams is the best advice now. 2 days is a very short time.
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: B_happy28]
#26724382 - 06/06/20 03:21 PM (3 years, 8 months ago) |
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Thanks for your answer!!!
Yeah, I should have stuck with just the 1:1. Hopefully we have luck!! And I had no clue that Black Kow was such crap (no pun intended) 😂
My jars looked contam free and they looked healthy and all white. First jar went in no problems layered it with tha substrate but It was only when I started shaking the second jar out into the substrate that one small chick looked slightly greenish in tinge. I scooped it out, and tossed my gloves. I figured that at that point that’s all I could do. Didn’t see anymore but my hope is that the myc are strong and healthy and Will win the fight.
My question is should I be concerned about 99% humidity?
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: Strainsfordaze]
#26724389 - 06/06/20 03:26 PM (3 years, 8 months ago) |
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Quote:
Strainsfordaze said: Patiences my friend. Also the tub doesn’t need to be covered with a bag. Mushrooms benefit from light. 2 days is no time to start stressing. Leave it alone for at least 7-10 days then start with your pinning triggers.
Thank you! I thought while doing a second spawning(This time to substrate), you also need darkness just like when spawning grain?
And my biggest question is, is it ok at 99% humidity and condensation? I suppose you saying to not freak out likely means that’s ok. Lol😂 As a noob, patience is a virtue!
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: Noob 99% humidity in bulk substrate monotub [Re: B_happy28]
#26724396 - 06/06/20 03:31 PM (3 years, 8 months ago) |
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The humidity is fine. It is because you have closed off most fae. When the colonization gets closer to 70-100% that’s when you incorporate more air exchange. Big pinning trigger. Read this. https://www.shroomery.org/forums/showflat.php/Number/23999053
Also... the condensation is showing that their is a difference between the tub temp and the room temp(because mycelium is generating heat) There is nothing wrong, unless it starts dripping and dropping heavily.
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: Strainsfordaze]
#26736712 - 06/11/20 12:09 PM (3 years, 8 months ago) |
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Quote:
Strainsfordaze said: The humidity is fine. It is because you have closed off most fae. When the colonization gets closer to 70-100% that’s when you incorporate more air exchange. Big pinning trigger. Read this. https://www.shroomery.org/forums/showflat.php/Number/23999053
Also... the condensation is showing that their is a difference between the tub temp and the room temp(because mycelium is generating heat) There is nothing wrong, unless it starts dripping and dropping heavily.
Here’s how the substrate is looking on day 7. Not quite fully consolidated but looks healthy! I see shimmery specks. Not sure if that’s moisture or something else but I know that’s a good sign, right?
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Re: Noob 99% humidity in bulk substrate monotub [Re: B_happy28]
#26737091 - 06/11/20 03:33 PM (3 years, 8 months ago) |
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The moisture on the surface is normal. Did you read the link?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Noob 99% humidity in bulk substrate monotub [Re: Strainsfordaze]
#26737237 - 06/11/20 04:51 PM (3 years, 8 months ago) |
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Don't take pictures of your plastic tub when you want questions asked about your substrate.
Take a good picture will help a lot
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Roger Clemency
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Registered: 03/23/20
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Re: Noob 99% humidity in bulk substrate monotub [Re: bodhisatta]
#26737298 - 06/11/20 05:24 PM (3 years, 8 months ago) |
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On your next tub definitely mix the spawn in evenly with the bulk sub. Layering makes things take a lot longer and has no benefit that i've ever seen.
Cubensis grow naturally on poop but they also only put out a mushroom or two and it happens randomly. We're trying to get wall to wall coverage on command. The grain provides the nutrients (plenty) and the bulk sub provides the water and easily colonizable (airy) base and surface area needed to grow a bunch of mushrooms.
Coir works great for this. Hpoo works great too, especially with some vermiculite, but will contaminate more easily.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: Strainsfordaze]
#26737976 - 06/11/20 09:03 PM (3 years, 8 months ago) |
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Quote:
Strainsfordaze said: The moisture on the surface is normal. Did you read the link?
Yes, I did read it. The droplets are good. I guess I was just hoping to hear that it all looks good, and if I should wait until it’s fully colonized. I hear and read different things. Some say wait until it pins, some say only 70% colonization, and some say 100%.
Thanks again for your reply
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: bodhisatta]
#26740584 - 06/12/20 08:50 PM (3 years, 8 months ago) |
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Quote:
bodhisatta said: Don't take pictures of your plastic tub when you want questions asked about your substrate.
Take a good picture will help a lot

I thought I’m not supposed to open it due to contamination risk unless I need to add moisture.
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: Roger Clemency]
#26740587 - 06/12/20 08:52 PM (3 years, 8 months ago) |
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Quote:
Roger Clemency said: On your next tub definitely mix the spawn in evenly with the bulk sub. Layering makes things take a lot longer and has no benefit that i've ever seen.
Cubensis grow naturally on poop but they also only put out a mushroom or two and it happens randomly. We're trying to get wall to wall coverage on command. The grain provides the nutrients (plenty) and the bulk sub provides the water and easily colonizable (airy) base and surface area needed to grow a bunch of mushrooms.
Coir works great for this. Hpoo works great too, especially with some vermiculite, but will contaminate more easily.
How can you tell I layered it? I’ve seen people say it can be done either way. I should’ve done just verm and coir. So are you saying I’m not gonna get a good flush?
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SporeFlake
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Re: Noob 99% humidity in bulk substrate monotub [Re: B_happy28]
#26740835 - 06/12/20 10:57 PM (3 years, 8 months ago) |
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Not trying to Hi-jack your thread here, but this is the first I've seen what I'm looking for. So am I to understand that is pure Coco/Verm Substrate? No Pasteurization needed? I know, I'm total newb.
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solipschism
Prodigal Son

Registered: 05/22/20
Posts: 26
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Re: Noob 99% humidity in bulk substrate monotub [Re: B_happy28]
#26740902 - 06/13/20 12:20 AM (3 years, 8 months ago) |
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Quote:
B_happy28 said:How can you tell I layered it? I’ve seen people say it can be done either way.
Sure, it can be done either way, but think about what you're trying to achieve.
Each grain is gonna start growing out mycelium in an approximate sphere until all the substrate is colonized. The best case scenario is where the grains are in a perfectly even distribution, because each grain's myc will meet its neighbours' at approximately the same time. The maximal distance any grain has to grow myc out before the sub is fully colonized is minimized, that way, so it takes the least time.
Think about bunching all your spawn up in one corner - the distance the myc has to grow is very large (all the way to the other end of the tub). That's the worst case. Layering spawn is somewhere between these two cases - the distance the myc has to grow before full colonization is half the distance between your furthest-apart neighboring layers of spawn. Unless the act of mixing evenly retards growth significantly (and it doesn't), layering is always going to be heavily suboptimal for colonization times.
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Apples in Mono
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Registered: 09/21/17
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Re: Noob 99% humidity in bulk substrate monotub [Re: B_happy28]
#26740943 - 06/13/20 01:05 AM (3 years, 8 months ago) |
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I'm thinking some mold might take over your substrate before too long here
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Roger Clemency
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Re: Noob 99% humidity in bulk substrate monotub [Re: Apples in Mono]
#26741541 - 06/13/20 10:21 AM (3 years, 8 months ago) |
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Hola
You can take the lid off during colonization without worry. I had a lil heater/fan in the room (cold basement in winter) so it would dry my tubs out, i'd mist twice a day during colonization...from like day 2 or 3. Mostly you shouldnt have to open it much though with normal conditions and properly made sub but it doesnt hurt at all. When you mixed in the spawn with the bulk you also tumbled thousand of mold spores and bacteria in the mix.
Your grains are what are most susceptible to allowing mold a place to germinate, but if those grains are fully colonized by mycelium (even after the shake when it looks like they're not, the inside is still filled with cube myc) if they're colonized they can protect themselves from bad stuff.
Coir offers no place for spores to germinate, even if you bucket TEK the coir or properly pasteurize, or just hydrate it with cold water. Manure however needs to be properly pasteurized or it seems to allow trich to germinate and intertwine with your cube myc. pretty readily.
I know you layered it because you said so There are some high karate growers that believe in layering but the times i've tried it those tubs took longer to colonize by several days to a week.
I think if you have weak mycelium the layering may help it stay stronger without such a shock and a need to grow back out so far before reconnecting with other strains to make a mass. Thats just a thought though.
SO ...will you take a clear picture of that thing so's we can all look and judge you 
Sporeflake, B's tub is not just coir/verm. But yes with that mix you can do whatever you like to hydrate the sub. Coir thats been heat treated in some way works best though.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: Roger Clemency]
#26742161 - 06/13/20 04:28 PM (3 years, 7 months ago) |
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Quote:
Roger Clemency said: Hola
You can take the lid off during colonization without worry. I had a lil heater/fan in the room (cold basement in winter) so it would dry my tubs out, i'd mist twice a day during colonization...from like day 2 or 3. Mostly you shouldnt have to open it much though with normal conditions and properly made sub but it doesnt hurt at all. When you mixed in the spawn with the bulk you also tumbled thousand of mold spores and bacteria in the mix.
Your grains are what are most susceptible to allowing mold a place to germinate, but if those grains are fully colonized by mycelium (even after the shake when it looks like they're not, the inside is still filled with cube myc) if they're colonized they can protect themselves from bad stuff.
Coir offers no place for spores to germinate, even if you bucket TEK the coir or properly pasteurize, or just hydrate it with cold water. Manure however needs to be properly pasteurized or it seems to allow trich to germinate and intertwine with your cube myc. pretty readily.
I know you layered it because you said so There are some high karate growers that believe in layering but the times i've tried it those tubs took longer to colonize by several days to a week.
I think if you have weak mycelium the layering may help it stay stronger without such a shock and a need to grow back out so far before reconnecting with other strains to make a mass. Thats just a thought though.
SO ...will you take a clear picture of that thing so's we can all look and judge you 
Sporeflake, B's tub is not just coir/verm. But yes with that mix you can do whatever you like to hydrate the sub. Coir thats been heat treated in some way works best though.
Oh, duh! Forgot I wrote that! I think my grain was well colonized so maybe that will help. I did the bucket pasteurization but only for a couple hours and then hung it to get it to field capacity. So with the poop when would Trich appear?? Hope this is a good picture!
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: Apples in Mono]
#26742164 - 06/13/20 04:28 PM (3 years, 7 months ago) |
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Quote:
Apples in Mono said: I'm thinking some mold might take over your substrate before too long here
What makes you think that? Based on the picture or the conditions I set up?
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B_happy28
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Re: Noob 99% humidity in bulk substrate monotub [Re: B_happy28]
#26744352 - 06/14/20 03:50 PM (3 years, 7 months ago) |
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Here’s another pic. My hygrometer fogged completely up and now I can’t tell the humidity. Can I stick a new one in there? Can I lay it on the mycelium or should I tape to the side?
What do you think? Is it ready to fruit? If not, should I add my pepper seedling heat map next to it? The temp has stayed around 73-74 for the 10 days it’s been colonizing.

When I’m ready to fruit I have polyfill to swap out for the micro pore tape. And then planned to fan 2x daily and mist the sides if humidity is needed.
Thanks for any help!
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