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DJ Ed
Mushroom Engineer


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Isolates in Agar - what next?
#26741157 - 06/13/20 06:38 AM (3 years, 7 months ago) |
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Hi shroomery,
I’ve been getting to grips this year with agar. Using a still air box, and moving very slowly, practising sterile technique, I’ve managed to creat quite a few plates without contamination, nd with rhizomorphic growth,which is apparently what we need to aim for when growing out Psilocybe cubensis. Please take a look at my plates, and help me out: what do I need to do next? I want to store some of the isolates - do I now need to create culture slants?
Cheers DJ Ed
-------------------- “It’s like when you see a mountain lion,” he suggested. “If you run, it will chase you. So you must stand your ground.” Michael Pollan: How To Change Your Mind “The problem is not to find the answer, it’s to face the answer.” Terence McKenna

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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Isolates in Agar - what next? [Re: DJ Ed]
#26741168 - 06/13/20 06:47 AM (3 years, 7 months ago) |
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None are isolates. You do whatever you want with them most people grow them out to make mushrooms and store the cultures too.
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aga080
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Re: Isolates in Agar - what next? [Re: bodhisatta]
#26741176 - 06/13/20 06:57 AM (3 years, 7 months ago) |
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you need to express the MS growth and then biopsy your favorite phenotypes. get growing. the agar looks awesome imo. nice work.
edit: actually furhter looking at your plates, i would replate maybe once or twice more before dropping wedges into grain. you want a nice full plate of rhizo without any cobweb creeping along with it.
hopefully some more experienced agar experts will chime in.
Edited by aga080 (06/13/20 06:59 AM)
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DJ Ed
Mushroom Engineer


Registered: 09/04/16
Posts: 2,326
Loc: UK
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Re: Isolates in Agar - what next? [Re: aga080]
#26741244 - 06/13/20 07:43 AM (3 years, 7 months ago) |
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Yeah, I was thinking along those lines. I don’t think I’m taking small enough agar wedges to remove all the sectoring; turns out after years of psychedelics and cannabis use, and yes too much alcohol, my hands really shake when I, trying to do delicate agar work!
So do you not think the right hand growth on the third photo could be put to grain yet?
Cheers DJ Ed
-------------------- “It’s like when you see a mountain lion,” he suggested. “If you run, it will chase you. So you must stand your ground.” Michael Pollan: How To Change Your Mind “The problem is not to find the answer, it’s to face the answer.” Terence McKenna

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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Isolates in Agar - what next? [Re: DJ Ed]
#26741252 - 06/13/20 07:49 AM (3 years, 7 months ago) |
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Any of it can go to grain
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DJ Ed
Mushroom Engineer


Registered: 09/04/16
Posts: 2,326
Loc: UK
Last seen: 1 month, 28 days
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Re: Isolates in Agar - what next? [Re: bodhisatta]
#26741255 - 06/13/20 07:51 AM (3 years, 7 months ago) |
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Quote:
bodhisatta said: None are isolates. You do whatever you want with them most people grow them out to make mushrooms and store the cultures too.
LOL I’m bloody useless at this! All this effort, and now I’m getting somewhere, it’s like, what now!!
And I’m still trying to learn the correct terms for things. When I wrote “isolates”, I was trying to get across what Roger Rabbit talks bout in his videos: sectoring. He gives various examples of what to look for, and takes slices to new agar plates, in order to remove this “sectoring”. When you get to only one sector in a dish, transfer that to grain.
So if you don’t mind explaining, what are “isolates”? Are they not single “sector” growths?
Cheers dude DJ Ed
BTW, my second grow using your Unmodified tub tek produced SPECTACULAR results: 325g coir. 35L tub. 4 rye grain jars PES Amazonian. Multi-spore syringe to first harvest 39 days. 758g harvest, 90.4% water: 73g equivalent dry weight. Nice one
-------------------- “It’s like when you see a mountain lion,” he suggested. “If you run, it will chase you. So you must stand your ground.” Michael Pollan: How To Change Your Mind “The problem is not to find the answer, it’s to face the answer.” Terence McKenna

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DJ Ed
Mushroom Engineer


Registered: 09/04/16
Posts: 2,326
Loc: UK
Last seen: 1 month, 28 days
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Re: Isolates in Agar - what next? [Re: bodhisatta]
#26741260 - 06/13/20 07:52 AM (3 years, 7 months ago) |
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Grains soaking
-------------------- “It’s like when you see a mountain lion,” he suggested. “If you run, it will chase you. So you must stand your ground.” Michael Pollan: How To Change Your Mind “The problem is not to find the answer, it’s to face the answer.” Terence McKenna

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redhandmat
Dude


Registered: 05/09/19
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Re: Isolates in Agar - what next? [Re: aga080]
#26741403 - 06/13/20 09:04 AM (3 years, 7 months ago) |
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Quote:
aga080 said: you need to express the MS growth and then biopsy your favorite phenotypes. get growing. the agar looks awesome imo. nice work.
edit: actually furhter looking at your plates, i would replate maybe once or twice more before dropping wedges into grain. you want a nice full plate of rhizo without any cobweb creeping along with it.
hopefully some more experienced agar experts will chime in.
He doesnt have any cobweb on his plates. Maybe you mean tomentose growth? And even that is fine tbh.
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