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OfflineLuminous7


Registered: 01/26/16
Posts: 1,417
Last seen: 1 hour, 46 minutes
Iz it possible to save dis tub? :(
    #26737504 - 06/11/20 06:28 PM (3 years, 8 months ago)

Is this Tub salvagable?
[5 qts ape, cvg, 4" sub depth, 66QT sterilite] .

My ape tubs keep contaminating on me lately, Is it possible I need to use more spawn?


Sub volume is about 24 qts, so spawning 5qts spawn would make it more of a 1:5 spawn sub ratio?

This is on the tenth day of incubation. :frown: it was looking good to.





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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,816
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Re: Iz it possible to save dis tub? :( [Re: Luminous7]
    #26737524 - 06/11/20 06:36 PM (3 years, 8 months ago)

Probably not with ape, way too slow. I’m on day 21 and still have only knots on mine.

You could try saving a little part of it by amputating but it probably won’t work.


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OfflineLuminous7


Registered: 01/26/16
Posts: 1,417
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Re: Iz it possible to save dis tub? :( [Re: A.k.a]
    #26737679 - 06/11/20 07:21 PM (3 years, 8 months ago)

Quote:

A.k.a said:
Probably not with ape, way too slow. I’m on day 21 and still have only knots on mine.

You could try saving a little part of it by amputating but it probably won’t work.




True. Ive never had much luck with amputations.

Tis a shame I guess :shrug: Onto the next.

Any opinions on spawn ratio? Seems every time ive used 5qts spawn instead of 6 ( i usually do 6) I have issues.


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OfflineA.k.aM
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Re: Iz it possible to save dis tub? :( [Re: Luminous7]
    #26738014 - 06/11/20 09:20 PM (3 years, 8 months ago)

That means you gotta work on producing clean spawn.

With clean spawn you can use a tiny bit if you want, but when it’s dirty you need a lot so it colonizes the sub before the mold can get established.


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LAGM2020


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OfflineLuminous7


Registered: 01/26/16
Posts: 1,417
Last seen: 1 hour, 46 minutes
Re: Iz it possible to save dis tub? :( [Re: A.k.a]
    #26738084 - 06/11/20 09:56 PM (3 years, 8 months ago)

Quote:

A.k.a said:
That means you gotta work on producing clean spawn.

With clean spawn you can use a tiny bit if you want, but when it’s dirty you need a lot so it colonizes the sub before the mold can get established.




I beleive my spawn is pretty good. Cleaned on agar, then knocked to rye or LC. 

I dont get contammed tubs often, But the past couple ape i did got infected.

I have been doing B+ as well , all of which have given 4 flushes before contams. I do the B+ and ape exactly the same within days of each other.

I work in a sab but ive gotten pretty good. I rarely see contamed plates or jars ( even off clone tissue plates rarely contams) so I thought my sterile tech was good.

I guess just be more careful, with lab work, take more precautions like showering before agar work and wearing clean clothes, all that jazz ...


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OfflineDigDug
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Registered: 05/05/20
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Last seen: 7 months, 25 days
Re: Iz it possible to save dis tub? :( [Re: Luminous7]
    #26738639 - 06/12/20 04:27 AM (3 years, 8 months ago)

In my experience, a 1:5 ratio is pretty low.  Try increasing your spawn ratio and see if you get better results.  The substrate should be able to colonize a bit faster with a higher ratio, decreasing its vulnerability to contaminants.


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OfflineLuminous7


Registered: 01/26/16
Posts: 1,417
Last seen: 1 hour, 46 minutes
Re: Iz it possible to save dis tub? :( [Re: DigDug]
    #26740076 - 06/12/20 05:02 PM (3 years, 8 months ago)

Quote:

DigDug said:
In my experience, a 1:5 ratio is pretty low.  Try increasing your spawn ratio and see if you get better results.  The substrate should be able to colonize a bit faster with a higher ratio, decreasing its vulnerability to contaminants.



This is my thought as well.

Guess ill focus on sterile tech practice more aswell.

:peace:


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