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SentinelFungi
Stranger
Registered: 04/05/20
Posts: 100
Last seen: 3 years, 3 months
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Contams? Pics of my first attempt at grains
#26737914 - 06/11/20 08:40 PM (3 years, 7 months ago) |
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Hi guys. I've got a grain jar I inoculated with 1ml of spore syringe on the 24th of May. Slow growth but I'm wondering if it's all good. The White area on the bottom looks healthy to me, but the upper layer looks different. Like the grains are coated in gypsum or something. The grains move freely, no signs of contams that I'm familiar with (not many).
Opinions?
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redhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
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Re: Contams? Pics of my first attempt at grains [Re: SentinelFungi]
#26738279 - 06/11/20 11:51 PM (3 years, 7 months ago) |
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Quote:
SentinelFungi said: Hi guys. I've got a grain jar I inoculated with 1ml of spore syringe on the 24th of May. Slow growth but I'm wondering if it's all good. The White area on the bottom looks healthy to me, but the upper layer looks different. Like the grains are coated in gypsum or something. The grains move freely, no signs of contams that I'm familiar with (not many).
Opinions?

The bottom white is definitely myc. But I think you have bacterial issues because of the wet and slimy look in some parts. Maybe something else riding along too. But why would you go syringe to grains? Its so risky, unless you know that your syringe is 100% contaminant free.
Im no expert, but Id recommend taking your syringe to agar. Get a clean culture on agar. PC your grain jars for 2-2.5 hours at 15 PSI. Then use the agar to inoculate your jars (instead of the syringe).
But lets see what people with more experience say.
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PMBastian
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Registered: 04/15/16
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Loc: Europe
Last seen: 2 years, 10 months
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Re: Contams? Pics of my first attempt at grains [Re: redhandmat]
#26738397 - 06/12/20 01:03 AM (3 years, 7 months ago) |
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I agree, I don't like the look of they Grey, slimy, wispy stuff on top.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Contams? Pics of my first attempt at grains [Re: redhandmat]
#26738402 - 06/12/20 01:07 AM (3 years, 7 months ago) |
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Quote:
SentinelFungi said: The White area on the bottom looks healthy to me, but the upper layer looks different. Like the grains are coated in gypsum or something.
Your grain prep could have been much better, I see lots of burst grains and wet spots. I cant say it's bacterial cause I see no clear indications of it being bacterial, wet grains happen, and pressed up against the glass grains happen as well and don't always get colonized, and if you say that the grains on top have something (growing?) on them that's not mycelium? Then throw that jar away as soon as possible and the fault would be your sterile Tek and perhaps not the spore solution. Focus on prepping grain that are always dry on the outside after inoculation. Gl

Quote:
redhandmat said: But why would you go syringe to grains? Its so risky, unless you know that your syringe is 100% contaminant free.
Spore solutions can never be 100% 'clean' unless grown and printed in sterile environment. Also 1ml of reasonably clean spore solution to a qt jar of perfectly prepped grain that are dry on the outside isn't that risky. But the grains in this case are obviously not very well prepped and
-------------------- Cakes inside Water Tub
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SentinelFungi
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Registered: 04/05/20
Posts: 100
Last seen: 3 years, 3 months
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Re: Contams? Pics of my first attempt at grains [Re: Mateja]
#26741705 - 06/13/20 12:18 PM (3 years, 7 months ago) |
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Okay, thanks for the help. That condensation is new and some of it might not be condensation but the glass pattern on the surface of the jars.
I prepped the grains following RR exactly and dried the grains to the point that there was no sticking or condensation in the jars after filling them and leaving them for a few hours. I guess maybe I'll throw them in the over for a few minutes as well next time.
Always room for improvement it seems.
Thanks again.
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