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Luminous7


Registered: 01/26/16
Posts: 1,417
Last seen: 1 hour, 46 minutes
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Iz it possible to save dis tub? :(
#26737504 - 06/11/20 06:28 PM (3 years, 8 months ago) |
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Is this Tub salvagable? [5 qts ape, cvg, 4" sub depth, 66QT sterilite] .
My ape tubs keep contaminating on me lately, Is it possible I need to use more spawn?
Sub volume is about 24 qts, so spawning 5qts spawn would make it more of a 1:5 spawn sub ratio?
This is on the tenth day of incubation. it was looking good to.

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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 day, 7 hours
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Re: Iz it possible to save dis tub? :( [Re: Luminous7]
#26737524 - 06/11/20 06:36 PM (3 years, 8 months ago) |
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Probably not with ape, way too slow. I’m on day 21 and still have only knots on mine.
You could try saving a little part of it by amputating but it probably won’t work.
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LAGM2020     
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Luminous7


Registered: 01/26/16
Posts: 1,417
Last seen: 1 hour, 46 minutes
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Re: Iz it possible to save dis tub? :( [Re: A.k.a]
#26737679 - 06/11/20 07:21 PM (3 years, 8 months ago) |
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Quote:
A.k.a said: Probably not with ape, way too slow. I’m on day 21 and still have only knots on mine.
You could try saving a little part of it by amputating but it probably won’t work.
True. Ive never had much luck with amputations.
Tis a shame I guess Onto the next.
Any opinions on spawn ratio? Seems every time ive used 5qts spawn instead of 6 ( i usually do 6) I have issues.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 day, 7 hours
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Re: Iz it possible to save dis tub? :( [Re: Luminous7]
#26738014 - 06/11/20 09:20 PM (3 years, 8 months ago) |
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That means you gotta work on producing clean spawn.
With clean spawn you can use a tiny bit if you want, but when it’s dirty you need a lot so it colonizes the sub before the mold can get established.
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LAGM2020     
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Luminous7


Registered: 01/26/16
Posts: 1,417
Last seen: 1 hour, 46 minutes
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Re: Iz it possible to save dis tub? :( [Re: A.k.a]
#26738084 - 06/11/20 09:56 PM (3 years, 8 months ago) |
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Quote:
A.k.a said: That means you gotta work on producing clean spawn.
With clean spawn you can use a tiny bit if you want, but when it’s dirty you need a lot so it colonizes the sub before the mold can get established.
I beleive my spawn is pretty good. Cleaned on agar, then knocked to rye or LC.
I dont get contammed tubs often, But the past couple ape i did got infected.
I have been doing B+ as well , all of which have given 4 flushes before contams. I do the B+ and ape exactly the same within days of each other.
I work in a sab but ive gotten pretty good. I rarely see contamed plates or jars ( even off clone tissue plates rarely contams) so I thought my sterile tech was good.
I guess just be more careful, with lab work, take more precautions like showering before agar work and wearing clean clothes, all that jazz ...
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DigDug
Cultivator


Registered: 05/05/20
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Re: Iz it possible to save dis tub? :( [Re: Luminous7]
#26738639 - 06/12/20 04:27 AM (3 years, 8 months ago) |
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In my experience, a 1:5 ratio is pretty low. Try increasing your spawn ratio and see if you get better results. The substrate should be able to colonize a bit faster with a higher ratio, decreasing its vulnerability to contaminants.
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Luminous7


Registered: 01/26/16
Posts: 1,417
Last seen: 1 hour, 46 minutes
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Re: Iz it possible to save dis tub? :( [Re: DigDug]
#26740076 - 06/12/20 05:02 PM (3 years, 8 months ago) |
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Quote:
DigDug said: In my experience, a 1:5 ratio is pretty low. Try increasing your spawn ratio and see if you get better results. The substrate should be able to colonize a bit faster with a higher ratio, decreasing its vulnerability to contaminants.
This is my thought as well.
Guess ill focus on sterile tech practice more aswell.
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