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OfflineLadyUstriess
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Is it ok to re-sterilize my substrate jars? * 1
    #26736529 - 06/11/20 10:41 AM (3 years, 8 months ago)

Yay my first post, please be gentle! Apologies ahead of time as this is a total n00b question, but here goes ...
Not realizing the delay in shipping spores (due to covid) was going to be > 2 weeks, I made the mistake of PC sterilizing my half-pint substrate jars before having my spores in hand.
Yes, rookie error but my question: is it ok to re-sterilize my jars when I (finally) receive the spores? Or is this considered a bad idea and I should start from scratch and chalk the expense up to my inexperience?
Thanks in advance!


--------------------
“I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.”
― Hunter S. Thompson



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InvisibleMr Solo Dolo


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Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess]
    #26736559 - 06/11/20 10:51 AM (3 years, 8 months ago)

If you cook them again it will throw off the water content.. Just use them or make more.. I'd highly suggest make more..

And just making sure, your talking about grain jars? Or some sort of coir verm mix (substrate)?


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OfflineLadyUstriess
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Re: Is it ok to re-sterilize my substrate jars? [Re: Mr Solo Dolo]
    #26736584 - 06/11/20 10:59 AM (3 years, 8 months ago)

Thanks for your response, and yes they are grain jars :sun:


--------------------
“I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.”
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OfflineGastronomicus
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Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess] * 1
    #26736590 - 06/11/20 11:03 AM (3 years, 8 months ago)

If you're doing grain use bigger jars than half-pints


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Offlinehazyhorse
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Re: Is it ok to re-sterilize my substrate jars? [Re: Gastronomicus]
    #26736727 - 06/11/20 12:18 PM (3 years, 8 months ago)

what size is your pressure cooker? if you're using half pints because your cooker is smaller, i'd recommend using pint jars. i have a 6qt pressure cooker & can fit 4 pint jars in there at a time, for a total of 2qts of grain. not great, but i don't mind having to do a few batches

as for recooking the grains... i've done it on some recent jars bc i fucked up the PC cycle. the grains got hella dry & are slow to colonize. you'll see better results just making a new batch of grains. you want grains to be relatively fresh. i'd say let them sit no more than a few days after PCing them initially. bodhisatta (a well respected member on here, if you haven't encountered his teks or posts yet) has mentioned it's better to just remake grain jars over letting them sit for 4 days or longer

also... just so you know, MS to grain is generally not recommended. it can work, but it's a real crapshoot as to if you'll get contamination or not


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OfflineLadyUstriess
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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
    #26736759 - 06/11/20 12:32 PM (3 years, 8 months ago)

Yes, I have a smaller 6 quart PC. Thanks, I really appreciate your feedback as I'm just beginning and this will be my first grow. All feedback is much appreciated!


--------------------
“I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.”
― Hunter S. Thompson



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Offlinehazyhorse
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Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess]
    #26736792 - 06/11/20 12:50 PM (3 years, 8 months ago)

sh, i feel your pain. the 6qts are totally usable & tbh i feel like it limits me from growing too much (which is a good thing... it would be easy to grow pounds just bc they are so fun to cultivate lol). i was originally just PCing one quart jar at a time bc i could only get one to fit sideways in the cooker, but i realized that i could fit 4 pint jars standing straight up. 4 pints = 2 quarts so i doubled my output (LOL) but being able to cook 2qt at a time was a huge jump. if you're just doing like shoebox/minimono stuff it's a great size for what you probably paid for it ($10 at a thrift store for mine)

pint jars are a great size for handling & colonizing. they will probably be less annoying to use than half pint jars would be for grain. make sure to measure the height of your PC though as idk your PCs specific dimensions. they just happen to work well in mine

honestly, if you already have a PC you should look into agar if you haven't already. you're already gonna be using a still air box & using grains... making agar plates & using a scalpel isn't really that much more complicated

even if you just end up doing MS to grain, good luck! it's a super fun hobby. you'll learn as you go. i legitimately cultivate just for the process bc it's so fun. getting to trip is a nice bonus, though (:




also, is the strangers to ourselves a modest mouse reference?


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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OfflineLadyUstriess
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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
    #26736850 - 06/11/20 01:22 PM (3 years, 8 months ago)

Thanks - more good info. I'll be looking in local thrifts for some full pint size jars.
I have been reading the teks on agar and think I will move to that in my next grow or so, I was thinking to start the simple old fashion way and move up from there, lol. I do love the cultivation aspect as I do in other areas, like gardening or kombucha, and am excited to get started on my first myco grow, cant' wait for my B+ spores to arrive :smile:

and yes, Modest Mouse fan here, from the old days so I prob would be better with a Long Drive or Lonesome West reference lol!
Thanks again!


--------------------
“I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.”
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InvisibleRoger Clemency
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Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess] * 1
    #26736881 - 06/11/20 01:37 PM (3 years, 8 months ago)

Theoretical history lesson - Germinating on agar is actually the true old fashioned way. Old Psylocybe Fanaticus came up with the PF Tek (maybe the idea of a spore syringe idk) as a way to grown indoors fairly reliably without needing to do sterile work. He was a bum!! Lol really though I think the pf tek has probably been great for mush cult in general. It’s simple enough not to scare off people that just want a couple trips and it works often enough to rope people in to the long game.


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Offlinehazyhorse
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Re: Is it ok to re-sterilize my substrate jars? [Re: Roger Clemency]
    #26736904 - 06/11/20 01:54 PM (3 years, 8 months ago)

Quote:

TastyLadyUstriess said:
Thanks - more good info. I'll be looking in local thrifts for some full pint size jars.
I have been reading the teks on agar and think I will move to that in my next grow or so, I was thinking to start the simple old fashion way and move up from there, lol. I do love the cultivation aspect as I do in other areas, like gardening or kombucha, and am excited to get started on my first myco grow, cant' wait for my B+ spores to arrive :smile:

and yes, Modest Mouse fan here, from the old days so I prob would be better with a Long Drive or Lonesome West reference lol!
Thanks again!





i feel you! starting simple is where i started too. there’s a lot that can overwhelm you with information when it comes to this hobby. but also don’t be intimidated by agar when you start it! it is very easy. i only day this as i was a little intimidated by doing agar for the first time bc so much information was being thrown at me through google searches

& right on!! my sig used to be a lyric from baby blue sedan. LCW is one of my favorite albums of all time, they are so good. their work in the 90s & early 2000s is just so insanely good


Quote:

Roger Clemency said:
Theoretical history lesson - Germinating on agar is actually the true old fashioned way. Old Psylocybe Fanaticus came up with the PF Tek (maybe the idea of a spore syringe idk) as a way to grown indoors fairly reliably without needing to do sterile work. He was a bum!! Lol really though I think the pf tek has probably been great for mush cult in general. It’s simple enough not to scare off people that just want a couple trips and it works often enough to rope people in to the long game.




lmao that’s too good. yeah, i guess the old fashioned way used to be rather inaccessible... if a lot of people didn’t start out with the PF tek, i wonder if we would be where we’re at with cultivation now. it’s a super accessible way to try it out & get your feet wet, or just grow the occasion small personal amount if you don’t wanna dive into bulk growing


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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Offlinemrpharmacist
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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
    #26783198 - 06/25/20 08:40 PM (3 years, 7 months ago)

Hi guys,

Another noob here!  with a similar question to the OP.  My story is slightly different.  I had a 6 year old print, which I turned into a syringe and inoculated my 12 jars (PF BRF Tek).  Unsurprisingly nothing grew - the print was way too old. 

One of my jars grew mould.  White fluff and then black spots.  I've chucked that one. 

My good (1 year old) spore syringe just arrived, but it's been 3-4 weeks since I PC'd my jars and inoculated with the dead spores.  I'm now ready to reinoculate my jars!

Should I bung the jars in the PC for another 90 mins to re-sterilize, just to be sure?  That was my plan as I don't have much time to make new cakes...

Mr P :smile:


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OfflineLogicaL ChaosM
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Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess]
    #26783369 - 06/25/20 10:20 PM (3 years, 7 months ago)

U have to re-do your jars because the grains will dry out before the spores arrive. Sorry :shrug:


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OfflinePhrontist
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Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
    #26783848 - 06/26/20 07:22 AM (3 years, 7 months ago)

Quote:

mrpharmacist said: 
I had a 6 year old print, which I turned into a syringe and inoculated my 12 jars (PF BRF Tek).  Unsurprisingly nothing grew - the print was way too old.




Nope. Someone in the pics thread grew a very nice tub from a 20 year old print a few weeks back.

If you’re putting spores straight to grain (skipping agar) you should expect it to fail and feel lucky if/when it doesn’t. And if you’re reusing old jars instead of making new ones, odds of success drop even lower.


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InvisibleLotKid
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Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess]
    #26783888 - 06/26/20 07:48 AM (3 years, 7 months ago)

If you sterilzed your jars properly then just set them on a shelf and use them when your spores arrive.

You can re-sterilze them if you want but, if done properly the first time, they should be good.


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InvisibleLotKid
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Re: Is it ok to re-sterilize my substrate jars? [Re: Mr Solo Dolo]
    #26783891 - 06/26/20 07:52 AM (3 years, 7 months ago)

Quote:

Mr Solo Dolo said:
If you cook them again it will throw off the water content.. Just use them or make more.. I'd highly suggest make more..

And just making sure, your talking about grain jars? Or some sort of coir verm mix (substrate)?



Re-sterilizing will not "throw off your water content"

Why make more? They've already been made. They dont go bad, immediatly.

OP, save your money and use what you have already made.

I'm guessing this is for pftek.

If sterilzed bags and jars went bad right away.... vendors couldnt sell them and ship them to ppl.

Think about it logically, ppl. C'mon...


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InvisibleLotKid
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Re: Is it ok to re-sterilize my substrate jars? [Re: LogicaL Chaos]
    #26783893 - 06/26/20 07:54 AM (3 years, 7 months ago)

Quote:

LogicaL Chaos said:
U have to re-do your jars because the grains will dry out before the spores arrive. Sorry :shrug:



I disagree.

Sometimes my grain masters sit for a month b4 wedges get dropped in them.


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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse] * 3
    #26783900 - 06/26/20 08:01 AM (3 years, 7 months ago)

Quote:

hazyhorse said:
what size is your pressure cooker? if you're using half pints because your cooker is smaller, i'd recommend using pint jars. i have a 6qt pressure cooker & can fit 4 pint jars in there at a time, for a total of 2qts of grain. not great, but i don't mind having to do a few batches

as for recooking the grains... i've done it on some recent jars bc i fucked up the PC cycle. the grains got hella dry & are slow to colonize. you'll see better results just making a new batch of grains. you want grains to be relatively fresh. i'd say let them sit no more than a few days after PCing them initially. bodhisatta (a well respected member on here, if you haven't encountered his teks or posts yet) has mentioned it's better to just remake grain jars over letting them sit for 4 days or longer

also... just so you know, MS to grain is generally not recommended. it can work, but it's a real crapshoot as to if you'll get contamination or not



I disagree with you saying..."let them sit no more than a few days"... i let mine sit longer than that on the reg.

And your statement about bod being well respected here... its really only noobs that hold him on pedistal.

The rest of us think he's an overrated hack with a bunch of shit info.

He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.


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InvisibleSmartattack
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Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid] * 1
    #26783914 - 06/26/20 08:10 AM (3 years, 7 months ago)

I regularly let jars sit for a couple weeks. When I do have issues, it's because of a culture issue not an older jar. Earlier this year I used a few jars PCed 8 months prior. They were a bit dry obviously but ended up making a decent tub.


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OfflineGastronomicus
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Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
    #26783956 - 06/26/20 08:35 AM (3 years, 7 months ago)

Quote:

LotKid said:

And your statement about bod being well respected here... its really only noobs that hold him on pedistal.

The rest of us think he's an overrated hack with a bunch of shit info.

He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.




:ilold:


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OfflinePhrontist
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Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
    #26783978 - 06/26/20 08:45 AM (3 years, 7 months ago)

Quote:

mrpharmacist said:
My good (1 year old) spore syringe just arrived, but it's been 3-4 weeks since I PC'd my jars and inoculated with the dead spores.  I'm now ready to reinoculate my jars!




Just to be clear, I wasn’t scoffing at using jars that had been properly sterilized and then sat for awhile. This guy already put liquid spores into his jars and now wants to drop a different syringe of liquid spores on top of it a month later.

I know you didn’t quote me for correction, but there were two different questions in here and I thought it was important to clarify which advice was for which situation.


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