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mushman1017
Stranger
Registered: 08/27/19
Posts: 168
Last seen: 2 years, 5 months
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My LC jars keep cracking!
#26736591 - 06/11/20 11:03 AM (3 years, 7 months ago) |
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So Ive been getting some "somewhat" shitty flushes over the past month or two. Recently Ive switched over to LC from G2G due to the quicker colonization times. I was getting ready to make another batch of LC, and when cleaning out the old vessels I noticed about 8 of the 12 of the jars Ive been using having round cracks at the bottom!

First of all, Im wondering if my non wall to wall flushes have been due to contamination by having the LC bottles possibly be contam'd from the cracks in the bottom. Would contam reduce pinset and create a lot of aborts?
Secondly... how the fuck am I cracking these? Its not from slamming it against anything... must be from heat/cold exchange or something but I usually let them sit in the PC for a while to cool off before pulling them out. The process I use is putting metal jar lids on the bottom of the PC, put my LC vessel in there and fill up with water till its about 1/3 up the way of the vessel.
Am I doing something wrong? Is there a better way to do it? Also, is there something else I can use that wont rust up the inside of the PC's other than the mason jar lids? Im using steel wool to clean it but it gets to be a pain in the ass, and it rusts all the bottom of the glass mason jars I use.
TIA.
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 15 hours, 42 minutes
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Re: My LC jars keep cracking! [Re: mushman1017]
#26736713 - 06/11/20 12:10 PM (3 years, 7 months ago) |
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contam can def reduce pinset & slow things down, but i'm not sure if that's specifically your problem here. were you getting wall to wall flushes before when you would g2g? are you using clones? or MS? is the agar your dropping in the LC completely clean? LC can be kinda sketchy if you aren't doing it properly. contams may be coming from the process of making your LC. are you using a syringe to inject the LC to grains? using a munchauzen style blenderless inoculation? contamination could be coming from how you're putting the LC into your grain jars. you could also just be dumping too much liquid in the jars & be opening the door for more bacterial contamination. a lot of factors
as for the cracks... all i can say is i have jars with similar "cracks" on the bottom. when i first noticed them, i thought they were cracked too. however, i use those "cracked" jars for spawn still all the time with no problems. i think it's just how the glass looks. i'm not sure exactly how it works, but i'd assume if you were getting contamination because of the cracking you'd be getting water leakage as well
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Antigov



Registered: 03/17/19
Posts: 792
Loc: Deep within the BibleBelt
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Re: My LC jars keep cracking! [Re: hazyhorse]
#26736757 - 06/11/20 12:32 PM (3 years, 7 months ago) |
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What kind of jars are you using?
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mushman1017
Stranger
Registered: 08/27/19
Posts: 168
Last seen: 2 years, 5 months
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Re: My LC jars keep cracking! [Re: hazyhorse]
#26736803 - 06/11/20 12:55 PM (3 years, 7 months ago) |
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Quote:
hazyhorse said: contam can def reduce pinset & slow things down, but i'm not sure if that's specifically your problem here. were you getting wall to wall flushes before when you would g2g? are you using clones? or MS? is the agar your dropping in the LC completely clean? LC can be kinda sketchy if you aren't doing it properly. contams may be coming from the process of making your LC. are you using a syringe to inject the LC to grains? using a munchauzen style blenderless inoculation? contamination could be coming from how you're putting the LC into your grain jars. you could also just be dumping too much liquid in the jars & be opening the door for more bacterial contamination. a lot of factors
as for the cracks... all i can say is i have jars with similar "cracks" on the bottom. when i first noticed them, i thought they were cracked too. however, i use those "cracked" jars for spawn still all the time with no problems. i think it's just how the glass looks. i'm not sure exactly how it works, but i'd assume if you were getting contamination because of the cracking you'd be getting water leakage as well
So the way I had been doing it is getting a clean transfer from agar, take a small cut of that and put it in my 2% malt extract mixture (although I just recently tried 1.5 grams per 500 ml and it seems to work a tiny bit better)... then after it is fully colonized I can start doing my transfers in my SAB. Im pretty cautious when doing transfers, i never put my hand or anything above the open lid, usually pour about 10-15ml worth of LC into each jar (usually do a quick swirl first prior to pouring) and when I pour into the mason jar I hold the lid up with my left hand at around 45 degree angle, or just a little more, just enough to pour my LC into and then close the lid after.
Ive had a handful of wall to wall flushes, but for the most part I would say its been about 30% empty space, 20% aborts and 50% flush. Obviously on the 2nd and 3rd flushes Ive been getting some decent monsters but im lucky to get 4-5 zips dry out of a 5 quart spawn tub after all the flushes are done. I know I should be getting close to double that, so its quite frustrating. This is also with clones, not MS.
As far as the cracks there are cracks in about 6 of them, the other 4 dont have that circular crack at all. On the shelf where I hold my LC while its innoculating there are def water marks so things arent completely sealed...
And this is the type of vessels I use :

The only other thing I can think of why my vessels have cracked is maybe putting them into the PC while the water is still pretty cold maybe the culprit? Im gonna run another batch right now and see if now that I let it warm up to room temp if that prevents it from happening.
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Justweed
Bobby's short shorts



Registered: 04/01/20
Posts: 555
Last seen: 3 months, 17 days
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Re: My LC jars keep cracking! [Re: mushman1017]
#26736828 - 06/11/20 01:10 PM (3 years, 7 months ago) |
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That's an indent from at the factory, the jar wasn't seated on the cooling rack properly.
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  Keep 'em high and tight guys....
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Alkeides
Child

Registered: 04/11/20
Posts: 61
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Re: My LC jars keep cracking! [Re: mushman1017]
#26736834 - 06/11/20 01:13 PM (3 years, 7 months ago) |
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Cadameda media bottles from amazon. I have some too. They're pretty low quality (wayyyyy below lab quality), but a good price for what they are provided they hold up. Lots of reviews mention them bursting in autoclaves suggesting they aren't borosilicate but personally I believe it's just manufacturing flaws. I'm not an industrial engineer but I believe they are formed by creating rough cylinder "blanks" of the two halves and a bottom round and those blanks are then either spun or blown into the bottle molds. Issues happen when the blanks arent kept in their uniformly heated glass state between original casting and final forming, which is why you can see the blank seam isn't concentric with the bottles final form on the bottom of the image you posted. I bet this is a manufacturer that got contracted for a bigger or new brand then they produced a ton of sub-par bottles that someone liquidated into the cadameda brand on amazon. The ones I have aren't cracked yet, but I doubt it's anything you're doing wrong.
I personally can't see the crack you're talking ab in that image, but glass is hard to take pics of. See if you can hilight what you're refering to with MS paint or something.
I also bet a hairline crack is fine and not a realy contam vector.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: My LC jars keep cracking! [Re: Alkeides]
#26736872 - 06/11/20 01:33 PM (3 years, 7 months ago) |
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Those are not cracks, it's a seam from the factory, damn near every media jar I own has that too
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The Mycologist
Explorer

Registered: 05/06/16
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Re: My LC jars keep cracking! [Re: natedawgnow]
#26736874 - 06/11/20 01:35 PM (3 years, 7 months ago) |
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If you are referencing that perfect circle on the bottom, they all have that.
-------------------- "That you are hereβthat life exists, and identity; That the powerful play goes on, and you will contribute a verse.β β Walt Whitman, Leaves of Grass

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mushman1017
Stranger
Registered: 08/27/19
Posts: 168
Last seen: 2 years, 5 months
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Re: My LC jars keep cracking! [Re: Alkeides]
#26736917 - 06/11/20 02:03 PM (3 years, 7 months ago) |
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Quote:
Lerman said: Cadameda media bottles from amazon. I have some too. They're pretty low quality (wayyyyy below lab quality), but a good price for what they are provided they hold up. Lots of reviews mention them bursting in autoclaves suggesting they aren't borosilicate but personally I believe it's just manufacturing flaws. I'm not an industrial engineer but I believe they are formed by creating rough cylinder "blanks" of the two halves and a bottom round and those blanks are then either spun or blown into the bottle molds. Issues happen when the blanks arent kept in their uniformly heated glass state between original casting and final forming, which is why you can see the blank seam isn't concentric with the bottles final form on the bottom of the image you posted. I bet this is a manufacturer that got contracted for a bigger or new brand then they produced a ton of sub-par bottles that someone liquidated into the cadameda brand on amazon. The ones I have aren't cracked yet, but I doubt it's anything you're doing wrong.
I personally can't see the crack you're talking ab in that image, but glass is hard to take pics of. See if you can hilight what you're refering to with MS paint or something.
I also bet a hairline crack is fine and not a realy contam vector.
Yes they are the ones from amazon... 3 for 15 I think?
Heres a better picture of it circled i red so you can see its not a part of the seam on the bottom as it is not uniformly cracked... goes into the outer edge of the bottle :

And also here is a picture of another jar that has inoculate in it that has NO CRACKS :

its def a crack and not just part of the way it looks as there are water remains underneath the vessel once I pick it up off the shelf.
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Alkeides
Child

Registered: 04/11/20
Posts: 61
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Re: My LC jars keep cracking! [Re: mushman1017]
#26737063 - 06/11/20 03:21 PM (3 years, 7 months ago) |
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Quote:
mushman1017 said:
Quote:
Lerman said: Cadameda media bottles from amazon. I have some too. They're pretty low quality (wayyyyy below lab quality), but a good price for what they are provided they hold up. Lots of reviews mention them bursting in autoclaves suggesting they aren't borosilicate but personally I believe it's just manufacturing flaws. I'm not an industrial engineer but I believe they are formed by creating rough cylinder "blanks" of the two halves and a bottom round and those blanks are then either spun or blown into the bottle molds. Issues happen when the blanks arent kept in their uniformly heated glass state between original casting and final forming, which is why you can see the blank seam isn't concentric with the bottles final form on the bottom of the image you posted. I bet this is a manufacturer that got contracted for a bigger or new brand then they produced a ton of sub-par bottles that someone liquidated into the cadameda brand on amazon. The ones I have aren't cracked yet, but I doubt it's anything you're doing wrong.
I personally can't see the crack you're talking ab in that image, but glass is hard to take pics of. See if you can hilight what you're refering to with MS paint or something.
I also bet a hairline crack is fine and not a realy contam vector.
Yes they are the ones from amazon... 3 for 15 I think?
Heres a better picture of it circled i red so you can see its not a part of the seam on the bottom as it is not uniformly cracked... goes into the outer edge of the bottle :

And also here is a picture of another jar that has inoculate in it that has NO CRACKS :

its def a crack and not just part of the way it looks as there are water remains underneath the vessel once I pick it up off the shelf.
Ok that's what I thought you were referring to. Here's my most used bottle with identical appearance and mine is definitely NOT cracked. If yours is cracked I guess it makes sense it would fail along a seam like that.
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