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OfflineGastronomicus
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Re: Is it ok to re-sterilize my substrate jars? [Re: Putrema]
    #26814725 - 07/10/20 09:46 AM (4 years, 6 months ago)

I think it's just that the smaller jar will colonize faster. So say you have some quart jars and you drop some agar into them. They won't be 100% colonized for 2 weeks at least, sometimes closer to 3. You use 100% colonized grains for G2G, so if you can throw that same agar wedge into a smaller jar it will be ready sooner and you can then expand it into the larger vessels.


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Offlinehazyhorse
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Re: Is it ok to re-sterilize my substrate jars? [Re: Putrema]
    #26814803 - 07/10/20 10:37 AM (4 years, 6 months ago)

i think smaller jars are just easier to handle for g2g. that, & iโ€™m assuming the colonize a lot faster than waiting for a whole quart jar to colonize

EDIT: lol didnโ€™t see this reply on the third page. but yeah


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Edited by hazyhorse (07/10/20 10:38 AM)

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InvisibleLotKid
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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
    #26816765 - 07/11/20 08:20 AM (4 years, 6 months ago)

Once you get the hang of cult... most of it boils down to personal preference.


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Offlinemrpharmacist
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Re: Is it ok to re-sterilize my substrate jars? [Re: Phrontist]
    #26820581 - 07/13/20 07:23 AM (4 years, 6 months ago)

Quote:

Phrontist said:
Quote:

mrpharmacist said:
My good (1 year old) spore syringe just arrived, but it's been 3-4 weeks since I PC'd my jars and inoculated with the dead spores.  I'm now ready to reinoculate my jars!




Just to be clear, I wasnโ€™t scoffing at using jars that had been properly sterilized and then sat for awhile. This guy already put liquid spores into his jars and now wants to drop a different syringe of liquid spores on top of it a month later.

I know you didnโ€™t quote me for correction, but there were two different questions in here and I thought it was important to clarify which advice was for which situation.





Kudos to the various posters in this thread for their advice.  Of my 12 jars, I re-did 8 of them from scratch,  and the other 4 were just re-sterilized in the PC.  I ran out of verm.

Of the 4 jars I didn't re-do, one of them has had contamination while the other 3 are almost a week behind the others in terms of mycelium growth.  It's an interesting observation from my first grow.  The freshly done jars are proving to be a better grow medium,  so it does seem worthwhile re-doing old jars if you have time and the materials.

...that said, one of my new jars has just started showing this green stuff (similar to one of the old jars which got contaminated).  I've now culled this one from the herd...

The daily jar examination has become a little bit nerve wracking!  How many will actually make it to the fruiting chamber.... !!??


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Offlinehazyhorse
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Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
    #26821167 - 07/13/20 01:51 PM (4 years, 6 months ago)

interesting!! i wonder if resterilizing PF tek jars vs grains had anything to do with it?? good luck with the contams tho. i hope you just got unlucky on a few jars & didnโ€™t get a bad syringe or something. seeing contams can be super disheartening


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Offlinemrpharmacist
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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
    #26822656 - 07/14/20 10:02 AM (4 years, 6 months ago)

Yeah I've thought the same about the syringe.  It was from a fairly random source.  I was at a mushroom conference in Australia, and this German guy did an excellent presentation which was a Beginners guide to growing mushrooms. 

He outlined the PF Tek method, and after his presentation he sat near me and my friend in the lecture theatre.  My mate asked him for advice on where to source good spores, and he pulled the syringe out his pocket and gave it to him with a smile and a wink!  I don't recall him saying what the spores were, but I'm assuming cubes of some description.

When my 7 year old spore print failed to grow I asked my mate to send me that 'mystery' syringe from the German bloke at the mushroom conference...  so that's what I'm working with on my first ever grow!  ;o)

He seemed pretty legit to be presenting at this Psychedelic mushroom event, so I'm feeling positive.  Hopefully the contaminated jars are just a few and are part of my learning curve as a newbie!

Will be interesting to share the fruits of my labour on here and get someone to ID them... 

This is one of those hobbies, where I'm saying to myself, Why didn't I start this years ago?!  haha!

Mr P :smile:

Edited by mrpharmacist (07/14/20 10:04 AM)

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