|
LadyUstriess
Strangers to Ourselves



Registered: 05/19/20
Posts: 34
Last seen: 1 year, 6 months
|
Is it ok to re-sterilize my substrate jars? 1
#26736529 - 06/11/20 10:41 AM (3 years, 7 months ago) |
|
|
Yay my first post, please be gentle! Apologies ahead of time as this is a total n00b question, but here goes ... Not realizing the delay in shipping spores (due to covid) was going to be > 2 weeks, I made the mistake of PC sterilizing my half-pint substrate jars before having my spores in hand. Yes, rookie error but my question: is it ok to re-sterilize my jars when I (finally) receive the spores? Or is this considered a bad idea and I should start from scratch and chalk the expense up to my inexperience? Thanks in advance!
-------------------- “I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.” ― Hunter S. Thompson
  
|
Mr Solo Dolo


Registered: 02/24/19
Posts: 243
Loc: U.S
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess]
#26736559 - 06/11/20 10:51 AM (3 years, 7 months ago) |
|
|
If you cook them again it will throw off the water content.. Just use them or make more.. I'd highly suggest make more..
And just making sure, your talking about grain jars? Or some sort of coir verm mix (substrate)?
--------------------
  LIFE'S A TRIP..  
|
LadyUstriess
Strangers to Ourselves



Registered: 05/19/20
Posts: 34
Last seen: 1 year, 6 months
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Mr Solo Dolo]
#26736584 - 06/11/20 10:59 AM (3 years, 7 months ago) |
|
|
Thanks for your response, and yes they are grain jars
-------------------- “I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.” ― Hunter S. Thompson
  
|
Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 3 hours, 45 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess] 1
#26736590 - 06/11/20 11:03 AM (3 years, 7 months ago) |
|
|
If you're doing grain use bigger jars than half-pints
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 25 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Gastronomicus]
#26736727 - 06/11/20 12:18 PM (3 years, 7 months ago) |
|
|
what size is your pressure cooker? if you're using half pints because your cooker is smaller, i'd recommend using pint jars. i have a 6qt pressure cooker & can fit 4 pint jars in there at a time, for a total of 2qts of grain. not great, but i don't mind having to do a few batches
as for recooking the grains... i've done it on some recent jars bc i fucked up the PC cycle. the grains got hella dry & are slow to colonize. you'll see better results just making a new batch of grains. you want grains to be relatively fresh. i'd say let them sit no more than a few days after PCing them initially. bodhisatta (a well respected member on here, if you haven't encountered his teks or posts yet) has mentioned it's better to just remake grain jars over letting them sit for 4 days or longer
also... just so you know, MS to grain is generally not recommended. it can work, but it's a real crapshoot as to if you'll get contamination or not
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
LadyUstriess
Strangers to Ourselves



Registered: 05/19/20
Posts: 34
Last seen: 1 year, 6 months
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
#26736759 - 06/11/20 12:32 PM (3 years, 7 months ago) |
|
|
Yes, I have a smaller 6 quart PC. Thanks, I really appreciate your feedback as I'm just beginning and this will be my first grow. All feedback is much appreciated!
-------------------- “I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.” ― Hunter S. Thompson
  
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 25 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess]
#26736792 - 06/11/20 12:50 PM (3 years, 7 months ago) |
|
|
sh, i feel your pain. the 6qts are totally usable & tbh i feel like it limits me from growing too much (which is a good thing... it would be easy to grow pounds just bc they are so fun to cultivate lol). i was originally just PCing one quart jar at a time bc i could only get one to fit sideways in the cooker, but i realized that i could fit 4 pint jars standing straight up. 4 pints = 2 quarts so i doubled my output (LOL) but being able to cook 2qt at a time was a huge jump. if you're just doing like shoebox/minimono stuff it's a great size for what you probably paid for it ($10 at a thrift store for mine)
pint jars are a great size for handling & colonizing. they will probably be less annoying to use than half pint jars would be for grain. make sure to measure the height of your PC though as idk your PCs specific dimensions. they just happen to work well in mine
honestly, if you already have a PC you should look into agar if you haven't already. you're already gonna be using a still air box & using grains... making agar plates & using a scalpel isn't really that much more complicated
even if you just end up doing MS to grain, good luck! it's a super fun hobby. you'll learn as you go. i legitimately cultivate just for the process bc it's so fun. getting to trip is a nice bonus, though (:
also, is the strangers to ourselves a modest mouse reference?
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
LadyUstriess
Strangers to Ourselves



Registered: 05/19/20
Posts: 34
Last seen: 1 year, 6 months
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
#26736850 - 06/11/20 01:22 PM (3 years, 7 months ago) |
|
|
Thanks - more good info. I'll be looking in local thrifts for some full pint size jars. I have been reading the teks on agar and think I will move to that in my next grow or so, I was thinking to start the simple old fashion way and move up from there, lol. I do love the cultivation aspect as I do in other areas, like gardening or kombucha, and am excited to get started on my first myco grow, cant' wait for my B+ spores to arrive
and yes, Modest Mouse fan here, from the old days so I prob would be better with a Long Drive or Lonesome West reference lol! Thanks again!
-------------------- “I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.” ― Hunter S. Thompson
  
|
Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess] 1
#26736881 - 06/11/20 01:37 PM (3 years, 7 months ago) |
|
|
Theoretical history lesson - Germinating on agar is actually the true old fashioned way. Old Psylocybe Fanaticus came up with the PF Tek (maybe the idea of a spore syringe idk) as a way to grown indoors fairly reliably without needing to do sterile work. He was a bum!! Lol really though I think the pf tek has probably been great for mush cult in general. It’s simple enough not to scare off people that just want a couple trips and it works often enough to rope people in to the long game.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 25 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Roger Clemency]
#26736904 - 06/11/20 01:54 PM (3 years, 7 months ago) |
|
|
Quote:
TastyLadyUstriess said: Thanks - more good info. I'll be looking in local thrifts for some full pint size jars. I have been reading the teks on agar and think I will move to that in my next grow or so, I was thinking to start the simple old fashion way and move up from there, lol. I do love the cultivation aspect as I do in other areas, like gardening or kombucha, and am excited to get started on my first myco grow, cant' wait for my B+ spores to arrive
and yes, Modest Mouse fan here, from the old days so I prob would be better with a Long Drive or Lonesome West reference lol! Thanks again!
i feel you! starting simple is where i started too. there’s a lot that can overwhelm you with information when it comes to this hobby. but also don’t be intimidated by agar when you start it! it is very easy. i only day this as i was a little intimidated by doing agar for the first time bc so much information was being thrown at me through google searches
& right on!! my sig used to be a lyric from baby blue sedan. LCW is one of my favorite albums of all time, they are so good. their work in the 90s & early 2000s is just so insanely good
Quote:
Roger Clemency said: Theoretical history lesson - Germinating on agar is actually the true old fashioned way. Old Psylocybe Fanaticus came up with the PF Tek (maybe the idea of a spore syringe idk) as a way to grown indoors fairly reliably without needing to do sterile work. He was a bum!! Lol really though I think the pf tek has probably been great for mush cult in general. It’s simple enough not to scare off people that just want a couple trips and it works often enough to rope people in to the long game.
lmao that’s too good. yeah, i guess the old fashioned way used to be rather inaccessible... if a lot of people didn’t start out with the PF tek, i wonder if we would be where we’re at with cultivation now. it’s a super accessible way to try it out & get your feet wet, or just grow the occasion small personal amount if you don’t wanna dive into bulk growing
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
mrpharmacist
Stranger



Registered: 05/20/20
Posts: 73
Last seen: 1 year, 1 month
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
#26783198 - 06/25/20 08:40 PM (3 years, 7 months ago) |
|
|
Hi guys,
Another noob here! with a similar question to the OP. My story is slightly different. I had a 6 year old print, which I turned into a syringe and inoculated my 12 jars (PF BRF Tek). Unsurprisingly nothing grew - the print was way too old.
One of my jars grew mould. White fluff and then black spots. I've chucked that one.
My good (1 year old) spore syringe just arrived, but it's been 3-4 weeks since I PC'd my jars and inoculated with the dead spores. I'm now ready to reinoculate my jars!
Should I bung the jars in the PC for another 90 mins to re-sterilize, just to be sure? That was my plan as I don't have much time to make new cakes...
Mr P
|
LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
Last seen: 1 hour, 13 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess]
#26783369 - 06/25/20 10:20 PM (3 years, 7 months ago) |
|
|
U have to re-do your jars because the grains will dry out before the spores arrive. Sorry
|
Phrontist
Determined



Registered: 07/17/13
Posts: 518
Last seen: 7 months, 11 days
|
Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
#26783848 - 06/26/20 07:22 AM (3 years, 6 months ago) |
|
|
Quote:
mrpharmacist said: I had a 6 year old print, which I turned into a syringe and inoculated my 12 jars (PF BRF Tek). Unsurprisingly nothing grew - the print was way too old.
Nope. Someone in the pics thread grew a very nice tub from a 20 year old print a few weeks back.
If you’re putting spores straight to grain (skipping agar) you should expect it to fail and feel lucky if/when it doesn’t. And if you’re reusing old jars instead of making new ones, odds of success drop even lower.
|
LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LadyUstriess]
#26783888 - 06/26/20 07:48 AM (3 years, 6 months ago) |
|
|
If you sterilzed your jars properly then just set them on a shelf and use them when your spores arrive.
You can re-sterilze them if you want but, if done properly the first time, they should be good.
|
LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Mr Solo Dolo]
#26783891 - 06/26/20 07:52 AM (3 years, 6 months ago) |
|
|
Quote:
Mr Solo Dolo said: If you cook them again it will throw off the water content.. Just use them or make more.. I'd highly suggest make more..
And just making sure, your talking about grain jars? Or some sort of coir verm mix (substrate)?
Re-sterilizing will not "throw off your water content"
Why make more? They've already been made. They dont go bad, immediatly.
OP, save your money and use what you have already made.
I'm guessing this is for pftek.
If sterilzed bags and jars went bad right away.... vendors couldnt sell them and ship them to ppl.
Think about it logically, ppl. C'mon...
|
LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LogicaL Chaos]
#26783893 - 06/26/20 07:54 AM (3 years, 6 months ago) |
|
|
Quote:
LogicaL Chaos said: U have to re-do your jars because the grains will dry out before the spores arrive. Sorry 
I disagree.
Sometimes my grain masters sit for a month b4 wedges get dropped in them.
|
LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse] 3
#26783900 - 06/26/20 08:01 AM (3 years, 6 months ago) |
|
|
Quote:
hazyhorse said: what size is your pressure cooker? if you're using half pints because your cooker is smaller, i'd recommend using pint jars. i have a 6qt pressure cooker & can fit 4 pint jars in there at a time, for a total of 2qts of grain. not great, but i don't mind having to do a few batches
as for recooking the grains... i've done it on some recent jars bc i fucked up the PC cycle. the grains got hella dry & are slow to colonize. you'll see better results just making a new batch of grains. you want grains to be relatively fresh. i'd say let them sit no more than a few days after PCing them initially. bodhisatta (a well respected member on here, if you haven't encountered his teks or posts yet) has mentioned it's better to just remake grain jars over letting them sit for 4 days or longer
also... just so you know, MS to grain is generally not recommended. it can work, but it's a real crapshoot as to if you'll get contamination or not
I disagree with you saying..."let them sit no more than a few days"... i let mine sit longer than that on the reg.
And your statement about bod being well respected here... its really only noobs that hold him on pedistal.
The rest of us think he's an overrated hack with a bunch of shit info.
He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.
|
Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid] 1
#26783914 - 06/26/20 08:10 AM (3 years, 6 months ago) |
|
|
I regularly let jars sit for a couple weeks. When I do have issues, it's because of a culture issue not an older jar. Earlier this year I used a few jars PCed 8 months prior. They were a bit dry obviously but ended up making a decent tub.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
|
Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 3 hours, 45 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
#26783956 - 06/26/20 08:35 AM (3 years, 6 months ago) |
|
|
Quote:
LotKid said:
And your statement about bod being well respected here... its really only noobs that hold him on pedistal.
The rest of us think he's an overrated hack with a bunch of shit info.
He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
|
Phrontist
Determined



Registered: 07/17/13
Posts: 518
Last seen: 7 months, 11 days
|
Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
#26783978 - 06/26/20 08:45 AM (3 years, 6 months ago) |
|
|
Quote:
mrpharmacist said: My good (1 year old) spore syringe just arrived, but it's been 3-4 weeks since I PC'd my jars and inoculated with the dead spores. I'm now ready to reinoculate my jars!
Just to be clear, I wasn’t scoffing at using jars that had been properly sterilized and then sat for awhile. This guy already put liquid spores into his jars and now wants to drop a different syringe of liquid spores on top of it a month later.
I know you didn’t quote me for correction, but there were two different questions in here and I thought it was important to clarify which advice was for which situation.
|
fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,269
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Phrontist] 1
#26784037 - 06/26/20 09:06 AM (3 years, 6 months ago) |
|
|
I’ve resterilized bacterial jars quite a few times.. as long as you catch them early on, it’s not a problem. They might lose a smidge of water content and be a tad slower than usual but if reinoculated with clean inoculate, it definitely works. Not sure who I might be replying to, just throwing it out there that it can be done.. I hate throwing away hard work.
Faht
|
metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 2 months
|
Re: Is it ok to re-sterilize my substrate jars? [Re: fahtster]
#26784058 - 06/26/20 09:13 AM (3 years, 6 months ago) |
|
|
I have 12 unused jars that are 6 months old and still show no sign of contamination.
--------------------
        
 
   
 BEHOLD 
        
|
Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 6 hours
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
#26784077 - 06/26/20 09:20 AM (3 years, 6 months ago) |
|
|
Quote:
LotKid said:
Quote:
Mr Solo Dolo said: If you cook them again it will throw off the water content.. Just use them or make more.. I'd highly suggest make more..
And just making sure, your talking about grain jars? Or some sort of coir verm mix (substrate)?
Re-sterilizing will not "throw off your water content"
Why make more? They've already been made. They dont go bad, immediatly.
OP, save your money and use what you have already made.
I'm guessing this is for pftek.
If sterilzed bags and jars went bad right away.... vendors couldnt sell them and ship them to ppl.
Think about it logically, ppl. C'mon...

On the odd occasion a jar has been sitting around for a bit I have re-sterilised and its always fine.
Endospores can come back from the dead so a quick blast in the PC will stop them re-animating.
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 25 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
#26786086 - 06/27/20 12:22 AM (3 years, 6 months ago) |
|
|
Quote:
LotKid said: I disagree with you saying..."let them sit no more than a few days"... i let mine sit longer than that on the reg.
yeah i def stand corrected on that. it totally makes sense they would stay sterile obviously, i just assumed they tend to dry out or something a bit too much if they sit for weeks
Quote:
LotKid said: And your statement about bod being well respected here... its really only noobs that hold him on pedistal.
The rest of us think he's an overrated hack with a bunch of shit info.
He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.
do people dislike bod on here? he's definitely not a god or anything, but i did find his teks/posts pretty helpful when i was starting out. i never really got the vibe people had problems with him based on what i've seen, but i don't use the forums that intensely. guess i've just never seen any drama
btw the penis envy tips in your sig was so clutch, i just happened to be starting a tub of some & it was great to see all that info compiled in one place vs. having to dig through dozens of threads to figure it out!!
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
shaggyp
California Hottboi



Registered: 12/27/12
Posts: 454
Last seen: 1 year, 9 months
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
#26786115 - 06/27/20 12:45 AM (3 years, 6 months ago) |
|
|
Like other people have said, properly sterilized jars with functional seals and filters should be fine.
But i do a quick 30 minutes on old jars and dont have moisture issues or contam issues that way.
-------------------- Burt Cocaine
|
LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
#26786536 - 06/27/20 07:08 AM (3 years, 6 months ago) |
|
|
Quote:
hazyhorse said:
Quote:
LotKid said: I disagree with you saying..."let them sit no more than a few days"... i let mine sit longer than that on the reg.
yeah i def stand corrected on that. it totally makes sense they would stay sterile obviously, i just assumed they tend to dry out or something a bit too much if they sit for weeks
Quote:
LotKid said: And your statement about bod being well respected here... its really only noobs that hold him on pedistal.
The rest of us think he's an overrated hack with a bunch of shit info.
He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.
do people dislike bod on here? he's definitely not a god or anything, but i did find his teks/posts pretty helpful when i was starting out. i never really got the vibe people had problems with him based on what i've seen, but i don't use the forums that intensely. guess i've just never seen any drama
btw the penis envy tips in your sig was so clutch, i just happened to be starting a tub of some & it was great to see all that info compiled in one place vs. having to dig through dozens of threads to figure it out!!
Yeah... i think bod might hold the site record for ppl putting him on ignore. And I mean that. Not just joking. If you've never seen any drama here then I hope it stays that way for you.
And dont let bod catch you saying that he's less than godly. You might get the wrath... 
Glad that you found my penis ramblings helpful
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 25 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
#26787848 - 06/27/20 05:19 PM (3 years, 6 months ago) |
|
|
Quote:
LotKid said: Yeah... i think bod might hold the site record for ppl putting him on ignore. And I mean that. Not just joking. If you've never seen any drama here then I hope it stays that way for you.
And dont let bod catch you saying that he's less than godly. You might get the wrath... 
Glad that you found my penis ramblings helpful 
no shit hahahahahaha that’s actually hilarious. the more you know. i guess i’ve just been lucky ;p i’ll sleep with one eye open...
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
mrpharmacist
Stranger



Registered: 05/20/20
Posts: 73
Last seen: 1 year, 1 month
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
#26806165 - 07/06/20 03:27 AM (3 years, 6 months ago) |
|
|
*** UPDATE ***
Thanks for all the comments. I managed to find some time and re-did my jars since they'd been inoculated twice with the old inactive spore solution. I ran out of materials, so 2 of the 12 jars didn't get redone. I just whacked them in the PC. Will be interesting to see how the 5 week old cakes perform versus the new ones I made.
The spore syringe I've used for Attempt 2 was one year old, and has been stored outside of a fridge (in cool dark place apparently). It's from a good source, but I don't know the strain...
8 days after inoculation I've got my first little spots of white fluffy-ness in 5 of the jars. I'm hoping the other 7 will begin displaying soon.
I was worried after a week with so signs. The temperature in the cupboard was around 20 deg C (70 F). I cranked it up to 25 deg C after 4 days, and happy to see things happening...
Is a 1 year old spore syringe a likely factor in a slow start? As a noob it was a lonnnng 8 days! haha! I was almost writing it off and searching for a fresh print!
One more question - Since I don't know the exact strain, which is the best thread, or who are the best people to consult for identification? I'm assuming cubensis of some description...
cheers, Mr P
Edited by mrpharmacist (07/06/20 06:36 AM)
|
metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 2 months
|
Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
#26806235 - 07/06/20 05:31 AM (3 years, 6 months ago) |
|
|
Time is the reason you're seeing growth. Not heat. Glad it's working out for you. Mostly all cubes require the same conditions, regardless of strain. If you're curious what the strain is you can post pics of your fruits to mushroom identification forum but I have a feeling people are just going to tell you: its a cube. Lol
--------------------
        
 
   
 BEHOLD 
        
|
Phrontist
Determined



Registered: 07/17/13
Posts: 518
Last seen: 7 months, 11 days
|
Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
#26806737 - 07/06/20 11:16 AM (3 years, 6 months ago) |
|
|
Quote:
mrpharmacist said: Is a 1 year old spore syringe a likely factor in a slow start?
Nope.
Variety is what you’re wondering about, not strain BTW.
|
F. 3
Flowing

Registered: 04/29/17
Posts: 192
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
#26806909 - 07/06/20 12:22 PM (3 years, 6 months ago) |
|
|
Quote:
LotKid said:
I disagree with you saying..."let them sit no more than a few days"... i let mine sit longer than that on the reg.
And your statement about bod being well respected here... its really only noobs that hold him on pedistal.
The rest of us think he's an overrated hack with a bunch of shit info.
He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.
smoke a
shits funny tho
|
LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Is it ok to re-sterilize my substrate jars? [Re: F. 3] 2
#26807546 - 07/06/20 05:24 PM (3 years, 6 months ago) |
|
|
I never touch the stuff...

|
F. 3
Flowing

Registered: 04/29/17
Posts: 192
|
Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
#26808270 - 07/07/20 04:30 AM (3 years, 6 months ago) |
|
|
observations only
Edited by F. 3 (07/07/20 04:31 AM)
|
B21
PeaceLoveShrooms



Registered: 04/09/20
Posts: 871
Loc: Derby City
Last seen: 3 months, 20 days
|
Re: Is it ok to re-sterilize my substrate jars? [Re: F. 3]
#26808292 - 07/07/20 05:16 AM (3 years, 6 months ago) |
|
|
--------------------
|
mrpharmacist
Stranger



Registered: 05/20/20
Posts: 73
Last seen: 1 year, 1 month
|
Re: Is it ok to re-sterilize my substrate jars? [Re: metaphoric]
#26810393 - 07/08/20 05:59 AM (3 years, 6 months ago) |
|
|
Well, 6 out of my 12 jars are colonising, but one of them ain't looking so good... 
First casualty of war I suspect? Incidentally it was one of the old,(previously inncolulated) jars which I just re-did in the PC, so had a higher chance of contamination)
Ive separated it from the others. That green shit doesn't look friendly. Destroy this one right?

Let's hope a few more of the jars start showing some action. Is it normal for some jars in a batch to start colonising earlier than others? We're on day 10. Six have begun (after 8 days), but the other five aren't showing anything. Cheers, Mr P
|
Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 3 hours, 45 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
#26810400 - 07/08/20 06:05 AM (3 years, 6 months ago) |
|
|
Yes that one is way past completely fucked
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
|
mrpharmacist
Stranger



Registered: 05/20/20
Posts: 73
Last seen: 1 year, 1 month
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Gastronomicus]
#26814238 - 07/10/20 04:07 AM (3 years, 6 months ago) |
|
|
Quote:
Gastronomicus said: Yes that one is way past completely fucked 
Yup, thought as much. That shade of green is natures sign of a nasty infection!
Well, the good news is a couple more of my jars are showing mycelium so I've got 7 now! The most recent ones took 12-13 days before displaying growth.
Let's hope the last 4 jars get a wiggle on...
No other signs of contamination.
|
w00tmycelium
Stranger


Registered: 11/28/19
Posts: 159
Last seen: 3 years, 4 months
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Gastronomicus]
#26814248 - 07/10/20 04:13 AM (3 years, 6 months ago) |
|
|
Quote:
Gastronomicus said: If you're doing grain use bigger jars than half-pints
Side question for this: I've had a lot of success with half pint grains for grain to grain transfer (after fully colonized and broken up) into larger jars with no contams so far.
What is the benefit of starting with the bigger jars?
|
Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 3 hours, 45 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: w00tmycelium]
#26814543 - 07/10/20 08:09 AM (3 years, 6 months ago) |
|
|
Nah you're right smaller jars are good for G2G. Just if you're going to use them for spawn use bigger ones.
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
|
Putrema
1shroomis1shroomer

Registered: 02/20/20
Posts: 124
Last seen: 1 year, 2 months
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Gastronomicus]
#26814712 - 07/10/20 09:41 AM (3 years, 6 months ago) |
|
|
I havent done g2g yet (although I threw some myceliated grains around on a very small scale with some results, then stalling). Why are smaller jars better for g2g, because its easier to handle the sab or what?
|
Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
Last seen: 3 hours, 45 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Putrema]
#26814725 - 07/10/20 09:46 AM (3 years, 6 months ago) |
|
|
I think it's just that the smaller jar will colonize faster. So say you have some quart jars and you drop some agar into them. They won't be 100% colonized for 2 weeks at least, sometimes closer to 3. You use 100% colonized grains for G2G, so if you can throw that same agar wedge into a smaller jar it will be ready sooner and you can then expand it into the larger vessels.
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 25 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Putrema]
#26814803 - 07/10/20 10:37 AM (3 years, 6 months ago) |
|
|
i think smaller jars are just easier to handle for g2g. that, & i’m assuming the colonize a lot faster than waiting for a whole quart jar to colonize
EDIT: lol didn’t see this reply on the third page. but yeah
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
Edited by hazyhorse (07/10/20 10:38 AM)
|
LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
#26816765 - 07/11/20 08:20 AM (3 years, 6 months ago) |
|
|
Once you get the hang of cult... most of it boils down to personal preference.
|
mrpharmacist
Stranger



Registered: 05/20/20
Posts: 73
Last seen: 1 year, 1 month
|
Re: Is it ok to re-sterilize my substrate jars? [Re: Phrontist]
#26820581 - 07/13/20 07:23 AM (3 years, 6 months ago) |
|
|
Quote:
Phrontist said:
Quote:
mrpharmacist said: My good (1 year old) spore syringe just arrived, but it's been 3-4 weeks since I PC'd my jars and inoculated with the dead spores. I'm now ready to reinoculate my jars!
Just to be clear, I wasn’t scoffing at using jars that had been properly sterilized and then sat for awhile. This guy already put liquid spores into his jars and now wants to drop a different syringe of liquid spores on top of it a month later.
I know you didn’t quote me for correction, but there were two different questions in here and I thought it was important to clarify which advice was for which situation.
Kudos to the various posters in this thread for their advice. Of my 12 jars, I re-did 8 of them from scratch, and the other 4 were just re-sterilized in the PC. I ran out of verm.
Of the 4 jars I didn't re-do, one of them has had contamination while the other 3 are almost a week behind the others in terms of mycelium growth. It's an interesting observation from my first grow. The freshly done jars are proving to be a better grow medium, so it does seem worthwhile re-doing old jars if you have time and the materials.
...that said, one of my new jars has just started showing this green stuff (similar to one of the old jars which got contaminated). I've now culled this one from the herd...
The daily jar examination has become a little bit nerve wracking! How many will actually make it to the fruiting chamber.... !!??
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 25 minutes
|
Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
#26821167 - 07/13/20 01:51 PM (3 years, 6 months ago) |
|
|
interesting!! i wonder if resterilizing PF tek jars vs grains had anything to do with it?? good luck with the contams tho. i hope you just got unlucky on a few jars & didn’t get a bad syringe or something. seeing contams can be super disheartening
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
mrpharmacist
Stranger



Registered: 05/20/20
Posts: 73
Last seen: 1 year, 1 month
|
Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
#26822656 - 07/14/20 10:02 AM (3 years, 6 months ago) |
|
|
Yeah I've thought the same about the syringe. It was from a fairly random source. I was at a mushroom conference in Australia, and this German guy did an excellent presentation which was a Beginners guide to growing mushrooms.
He outlined the PF Tek method, and after his presentation he sat near me and my friend in the lecture theatre. My mate asked him for advice on where to source good spores, and he pulled the syringe out his pocket and gave it to him with a smile and a wink! I don't recall him saying what the spores were, but I'm assuming cubes of some description.
When my 7 year old spore print failed to grow I asked my mate to send me that 'mystery' syringe from the German bloke at the mushroom conference... so that's what I'm working with on my first ever grow! ;o)
He seemed pretty legit to be presenting at this Psychedelic mushroom event, so I'm feeling positive. Hopefully the contaminated jars are just a few and are part of my learning curve as a newbie!
Will be interesting to share the fruits of my labour on here and get someone to ID them...
This is one of those hobbies, where I'm saying to myself, Why didn't I start this years ago?! haha!
Mr P
Edited by mrpharmacist (07/14/20 10:04 AM)
|
|