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InvisiblefahtsterM
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Re: Is it ok to re-sterilize my substrate jars? [Re: Phrontist] * 1
    #26784037 - 06/26/20 09:06 AM (3 years, 6 months ago)

I’ve resterilized bacterial jars quite a few times.. as long as you catch them early on, it’s not a problem.  They might lose a smidge of water content and be a tad slower than usual but if reinoculated with clean inoculate, it definitely works.  Not sure who I might be replying to, just throwing it out there that it can be done.. I hate throwing away hard work.

Faht


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Offlinemetaphoric
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Re: Is it ok to re-sterilize my substrate jars? [Re: fahtster]
    #26784058 - 06/26/20 09:13 AM (3 years, 6 months ago)

I have 12 unused jars that are 6 months old and still show no sign of contamination.


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
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OfflineEdmunter
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Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
    #26784077 - 06/26/20 09:20 AM (3 years, 6 months ago)

Quote:

LotKid said:
Quote:

Mr Solo Dolo said:
If you cook them again it will throw off the water content.. Just use them or make more.. I'd highly suggest make more..

And just making sure, your talking about grain jars? Or some sort of coir verm mix (substrate)?



Re-sterilizing will not "throw off your water content"

Why make more? They've already been made. They dont go bad, immediatly.

OP, save your money and use what you have already made.

I'm guessing this is for pftek.

If sterilzed bags and jars went bad right away.... vendors couldnt sell them and ship them to ppl.

Think about it logically, ppl. C'mon...





:whathesaid:

On the odd occasion a jar has been sitting around for a bit I have re-sterilised and its always fine.

Endospores can come back from the dead so a quick blast in the PC will stop them re-animating.


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Offlinehazyhorse
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Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
    #26786086 - 06/27/20 12:22 AM (3 years, 6 months ago)

Quote:

LotKid said:
I disagree with you saying..."let them sit no more than a few days"... i let mine sit longer than that on the reg.




yeah i def stand corrected on that. it totally makes sense they would stay sterile obviously, i just assumed they tend to dry out or something a bit too much if they sit for weeks

Quote:

LotKid said:
And your statement about bod being well respected here... its really only noobs that hold him on pedistal.

The rest of us think he's an overrated hack with a bunch of shit info.

He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.




do people dislike bod on here? he's definitely not a god or anything, but i did find his teks/posts pretty helpful when i was starting out. i never really got the vibe people had problems with him based on what i've seen, but i don't use the forums that intensely. guess i've just never seen any drama




btw the penis envy tips in your sig was so clutch, i just happened to be starting a tub of some & it was great to see all that info compiled in one place vs. having to dig through dozens of threads to figure it out!!


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Offlineshaggyp
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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
    #26786115 - 06/27/20 12:45 AM (3 years, 6 months ago)

Like other people have said, properly sterilized jars with functional seals and filters should be fine.

But i do a quick 30 minutes on old jars and dont have moisture issues or contam issues that way.


--------------------
Burt Cocaine


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InvisibleLotKid
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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
    #26786536 - 06/27/20 07:08 AM (3 years, 6 months ago)

Quote:

hazyhorse said:
Quote:

LotKid said:
I disagree with you saying..."let them sit no more than a few days"... i let mine sit longer than that on the reg.




yeah i def stand corrected on that. it totally makes sense they would stay sterile obviously, i just assumed they tend to dry out or something a bit too much if they sit for weeks

Quote:

LotKid said:
And your statement about bod being well respected here... its really only noobs that hold him on pedistal.

The rest of us think he's an overrated hack with a bunch of shit info.

He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.




do people dislike bod on here? he's definitely not a god or anything, but i did find his teks/posts pretty helpful when i was starting out. i never really got the vibe people had problems with him based on what i've seen, but i don't use the forums that intensely. guess i've just never seen any drama




btw the penis envy tips in your sig was so clutch, i just happened to be starting a tub of some & it was great to see all that info compiled in one place vs. having to dig through dozens of threads to figure it out!!



Yeah... i think bod might hold the site record for ppl putting him on ignore. And I mean that. Not just joking. If you've never seen any drama here then I hope it stays that way for you.

And dont let bod catch you saying that he's less than godly. You might get the wrath... :paranoid:

Glad that you found my penis ramblings helpful :rockon:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


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Offlinehazyhorse
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Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
    #26787848 - 06/27/20 05:19 PM (3 years, 6 months ago)

Quote:

LotKid said:
Yeah... i think bod might hold the site record for ppl putting him on ignore. And I mean that. Not just joking. If you've never seen any drama here then I hope it stays that way for you.

And dont let bod catch you saying that he's less than godly. You might get the wrath... :paranoid:

Glad that you found my penis ramblings helpful :rockon:




no shit hahahahahaha that’s actually hilarious. the more you know. i guess i’ve just been lucky ;p i’ll sleep with one eye open...


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Offlinemrpharmacist
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Re: Is it ok to re-sterilize my substrate jars? [Re: hazyhorse]
    #26806165 - 07/06/20 03:27 AM (3 years, 6 months ago)

*** UPDATE ***

Thanks for all the comments.  I managed to find some time and re-did my jars since they'd been inoculated twice with the old inactive spore solution.  I ran out of materials, so 2 of the 12 jars didn't get redone.  I just whacked them in the PC.  Will be interesting to see how the 5 week old cakes perform versus the new ones I made.

The spore syringe I've used for Attempt 2 was one year old, and has been stored outside of a fridge (in cool dark place apparently).    It's from a good source, but I don't know the strain...

8 days after inoculation I've got my first little spots of white fluffy-ness in 5 of the jars.  I'm hoping the other 7 will begin displaying soon. 

I was worried after a week with so signs. The temperature in the cupboard was around 20 deg C (70 F).  I cranked it up to 25 deg C after 4 days, and happy to see things happening...

Is a 1 year old spore syringe a likely factor in a slow start?  As a noob it was a lonnnng 8 days! haha!  I was almost writing it off and searching for a fresh print! 

One more question - Since I don't know the exact strain, which is the best thread, or who are the best people to consult for identification?  I'm assuming cubensis of some description...

cheers,
Mr P :smile:


Edited by mrpharmacist (07/06/20 06:36 AM)


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Offlinemetaphoric
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Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
    #26806235 - 07/06/20 05:31 AM (3 years, 6 months ago)

Time is the reason you're seeing growth. Not heat. Glad it's working out for you. Mostly all cubes require the same conditions, regardless of strain. If you're curious what the strain is you can post pics of your fruits to mushroom identification forum but I have a feeling people are just going to tell you: its a cube. Lol


--------------------
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
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OfflinePhrontist
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Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
    #26806737 - 07/06/20 11:16 AM (3 years, 6 months ago)

Quote:

mrpharmacist said:
Is a 1 year old spore syringe a likely factor in a slow start?




Nope.

Variety is what you’re wondering about, not strain BTW.


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InvisibleF. 3
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Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
    #26806909 - 07/06/20 12:22 PM (3 years, 6 months ago)

Quote:

LotKid said:

I disagree with you saying..."let them sit no more than a few days"... i let mine sit longer than that on the reg.

And your statement about bod being well respected here... its really only noobs that hold him on pedistal.

The rest of us think he's an overrated hack with a bunch of shit info.

He can create and organize hyperlinks like a mofo. But other than that he just regurgitates other ppls hard word... when he's not talking out his ass.





smoke a :bongload:

shits funny tho


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InvisibleLotKid
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Re: Is it ok to re-sterilize my substrate jars? [Re: F. 3] * 2
    #26807546 - 07/06/20 05:24 PM (3 years, 6 months ago)

I never touch the stuff...



--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:


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InvisibleF. 3
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Re: Is it ok to re-sterilize my substrate jars? [Re: LotKid]
    #26808270 - 07/07/20 04:30 AM (3 years, 6 months ago)

observations only

:rockon:


Edited by F. 3 (07/07/20 04:31 AM)


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OfflineB21
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Re: Is it ok to re-sterilize my substrate jars? [Re: F. 3]
    #26808292 - 07/07/20 05:16 AM (3 years, 6 months ago)



--------------------


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Offlinemrpharmacist
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Re: Is it ok to re-sterilize my substrate jars? [Re: metaphoric]
    #26810393 - 07/08/20 05:59 AM (3 years, 6 months ago)

Well, 6 out of my 12 jars are colonising, but one of them ain't looking so good... :tongue:

First casualty of war I suspect?  Incidentally it was one of the old,(previously inncolulated) jars which I just re-did in the PC,  so had a higher chance of contamination)

Ive separated it from the others. That green shit doesn't look friendly.  Destroy this one right?



Let's hope a few more of the jars start showing some action.  Is it normal for some jars in a batch to start colonising earlier than others?  We're on day 10.  Six have begun (after 8 days), but the other five aren't showing anything. 
Cheers,
Mr P


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OfflineGastronomicus
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Re: Is it ok to re-sterilize my substrate jars? [Re: mrpharmacist]
    #26810400 - 07/08/20 06:05 AM (3 years, 6 months ago)

Yes that one is way past completely fucked :puke:


--------------------
Make my Funk the P Funk, I wants to get Funked up

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Offlinemrpharmacist
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Re: Is it ok to re-sterilize my substrate jars? [Re: Gastronomicus]
    #26814238 - 07/10/20 04:07 AM (3 years, 6 months ago)

Quote:

Gastronomicus said:
Yes that one is way past completely fucked :puke:




Yup, thought as much.  That shade of green is natures sign of a nasty infection! 

Well, the good news is a couple more of my jars are showing mycelium so I've got 7 now!  The most recent ones took 12-13 days before displaying growth.

Let's hope the last 4 jars get a wiggle on...

No other signs of contamination. :tongue2:


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Offlinew00tmycelium
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Re: Is it ok to re-sterilize my substrate jars? [Re: Gastronomicus]
    #26814248 - 07/10/20 04:13 AM (3 years, 6 months ago)

Quote:

Gastronomicus said:
If you're doing grain use bigger jars than half-pints




Side question for this:  I've had a lot of success with half pint grains for grain to grain transfer (after fully colonized and broken up) into larger jars with no contams so far.

What is the benefit of starting with the bigger jars?


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OfflineGastronomicus
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Re: Is it ok to re-sterilize my substrate jars? [Re: w00tmycelium]
    #26814543 - 07/10/20 08:09 AM (3 years, 6 months ago)

Nah you're right smaller jars are good for G2G. Just if you're going to use them for spawn use bigger ones.


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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OfflinePutrema
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Re: Is it ok to re-sterilize my substrate jars? [Re: Gastronomicus]
    #26814712 - 07/10/20 09:41 AM (3 years, 6 months ago)

I havent done g2g yet (although I threw some myceliated grains around on a very small scale with some results, then stalling). Why are smaller jars better for g2g, because its easier to handle the sab or what?


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