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Offlinemushpunx
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LI vs LC
    #26736005 - 06/11/20 04:50 AM (3 years, 8 months ago)

Besides being able to grow a lot of it, is there a benefit to LC over LI that I'm not
aware of, when skipping G2G and inoculating large spawn bags with liquids?

I blend agar wedges in water in an Eberbach and use it fresh.

Alternatively I could use the LI to inoculate LC broth and let it spin for a few days on a stir plate before using it.
But I don't see any additional benefit to this for what I'm doing.
Maybe LC would colonize faster, I dunno.

It's early in the morning. :lol:

Thoughts?



Edited by mushpunx (06/12/20 05:26 AM)


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OfflineGan
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Re: LI vs LC [Re: mushpunx]
    #26736112 - 06/11/20 06:57 AM (3 years, 8 months ago)

One benefit I could see (although I could definitely be wrong; just starting LC) is that LC allows to expand the culture more from less material.

The amount of live myc in the LI solution is as little or as much as you put in it. It wont really grow much once placed in the sterilized aqueous environment. Please correct me on that if I'm wrong though. I have almost no experience with LI.

However, in the LC you can put a tiny, non-visible spec in there (via Josex biopsy method) and it will grow into enough myc to inoculate a large amount of spawn. So, theoretically, one agar plate could create absurd amounts of LC if you're just using a biopsy method. Whereas one plate of myc can only make as much LI as is on the plate. If that makes any sense lol.

Although, with that thinking you could just use your master plate to make an absurd amount of slave plates via biopsy method and use those slave plates for crazy amounts of LI. Kinda the same premise. But, I could be wrong lol. I'm still new to liquid methods of inoculating but I just wanted to chime in my thoughts. I'm interested to see the answers as well.


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OfflineA.k.aM
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Re: LI vs LC [Re: Gan]
    #26736163 - 06/11/20 07:35 AM (3 years, 8 months ago)

I don’t have tons of experience with them but from the stuff I’ve messed with li from grain or the surface of a plate is just like smaller amounts of lc.


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