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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: AGAR | Please help me identify what's on my plates! [Re: sinubrius]
    #26735985 - 06/11/20 04:24 AM (3 years, 8 months ago)

Quote:

sinubrius said:
There's so much constructive info here, thanks heaps for your help!!

For reference, I pc my plates at 15psi for at least 25 minutes at at most 35 minutes using a presto 23qt, plates are above water line on multiple trivets and I use around 2qts of water, with no problems with water loss.

I leave the plates in the pc over night once it's off the heat without opening it to help combat the condensation. Majority of my pasty plates (all the ones you see here, incl. plastic ones) are fitted with SFDs that are siliconed on with RTV over a single 1/4" hole.

Other plates I've done with these swabs have shown something similar, or have just bacced out entirely after showing no growth for an extended period of time. I have a bunch of clean cultures of this mold and some of them I opened and had a poke around, no bluing so far as I can tell. It also doesn't feel like any mycelium I've touched in the past (old PF cakes, things like that).

Plates that I've left aside as control plates to spec out any potential contam never show any signs of growth before I either use them or turf them to use the container again for a different recipe, but I keep the control plate around for a week at least, even if I've already used the other plates beforehand, so that if something pops up on the control I can keep an eye out for it in the others.

Quote:

Lerman said:
Also you won't be able to access the shroomery trade forum until you have 50 posts and have been a member for 90 days here.




Do you reckon there's any forum mods I could message for potential inclusion before this threshold? I've had this account for a while now, 2 years atleast. I just try to lurk because I'm not that experienced yet so I can't render much help, and I definitely can't just spam the forums. If you don't think it's possible, fair enough, just figured I'd ask if something like that can happen in anyone's experience here on the forums. :smile:

Really appreciate the help!!!!!!

**EDIT - Also, no photos of the original culture, but it looked identical to above photos. I've got no doubt that there was never any mycelia there in the first place, unfortunately.

- Not quite sure what you mean by germination plates - do you mean the plate with the original culture that I took the mold from, or any plate I have that shows mycelia? (No plates so far have shown what I can determine as mycelia). Sorry if this is nooby!!




Yeah it doesn't sound like its your sterile tek. Id recommend you to keep trying on the swabs that you have an be active in the forums. There are many posts on cleaning up a culture, and already in this thread you have had some recommendations. Germination plate is the very first plates you swabbed, before transferring.

So you could cut small pieces or the entire cotton swab in plates so you get as many plates going at once as possible. And then on those plates try different methods of cleaning a culture. IT could be that your swabs are so riddled with bacteria and molds that it will be impossible to get a clean culture. But you will still get some experience, and it will give you a reason to keep on posting in the forums.

Sooner or later you will reach the 50 post minimum. After all the trade forum is for active posters in the community, so I doubt moderators would make exceptions to rules that apply for all. So hang in there, be active here and with your culture and things will work out sooner or later.


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OfflineZena
Stranger
Registered: 05/27/20
Posts: 26
Last seen: 1 year, 8 months
Re: AGAR | Please help me identify what's on my plates! [Re: sinubrius]
    #26736078 - 06/11/20 06:14 AM (3 years, 8 months ago)

Quote:

sinubrius said:

- Not quite sure what you mean by germination plates - do you mean the plate with the original culture that I took the mold from, or any plate I have that shows mycelia? (No plates so far have shown what I can determine as mycelia). Sorry if this is nooby!!





The plate you originally inoculated/used your swab on.


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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


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