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OfflineHurrakrayne
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Registered: 06/26/17
Posts: 217
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My Second Agar Grow * 1
    #26701575 - 05/28/20 11:12 AM (3 years, 7 months ago)

Hows it going everyone??


So i started my second try at growing with agar. My first try was with Pasty's mini rounds and i didnt have great success. Im using bods tek this time around.

So this time i went with  no pour and still used BRF. The agar this time looked 10 times better than last time so thats a plus. I have 20 plates poured as well. I also sanitized alot more and tried to be more thorough.

Gonna post photos when i start to get mycelium on the plates.

Peace!


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Offlinejay.achS
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26702454 - 05/28/20 06:44 PM (3 years, 7 months ago)

Good luck, man! 

What went wrong the first time?


--------------------
“One's destination is never a place, but a new way of seeing things.”  - Henry Miller

LAGM v. 2.023
LAGM2021


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: jay.ach]
    #26708554 - 05/31/20 06:36 AM (3 years, 7 months ago)

Bad technique. I don't think I was sanitizing as much as I should or I did the grains wrong. I had beautiful agar growth and transferred to grain and started and it started colonizing then everything turned black and greenish.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26708582 - 05/31/20 06:59 AM (3 years, 7 months ago)

Day 3..
This is what I have so far. A little condensation but I don't think that should be too much of an issue.
The 2 on the outside of the SAB had the most condensation so I figured I would knock them up in open air and see what happens and they also have some mycellium starting. Everything is looking good so far. Will post more when pics when I can.



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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26713752 - 06/02/20 11:02 AM (3 years, 7 months ago)

Day 5..
Got more growth. The temps dropped in my area a bit. Mid 50s overnight. Hoping that won't be too much of an issue. The loner is the one I nocced in open air. Seems to be good so far.




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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26721053 - 06/05/20 06:28 AM (3 years, 7 months ago)

Day 7...
Looking good. One plate I nocced in open air contammed but the other one is thriving and no other contams yet.



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InvisibleTrippypete
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26721059 - 06/05/20 06:34 AM (3 years, 7 months ago)

Awesome. I tried making some plates last night but I accidentally bought/used barley extract and they came out green. So I'm not sure how easy it will be to spot mold in them. I guess I don't follow directions well. It was my first time so i definitely learned from it.


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Intolerance will not be tolerated.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Trippypete]
    #26733626 - 06/10/20 06:52 AM (3 years, 7 months ago)

Day 12...

Mycellium seems to be taking hold and getting thicker by the day.



Edited by Hurrakrayne (06/10/20 06:53 AM)


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26736040 - 06/11/20 05:41 AM (3 years, 7 months ago)

Gonna do some transfers today. I don't have anymore empty plates so I'm gonna use the cups that pizza places out hot sauce in. Lol. Idk the proper name for them. 2 more plate have contams so I wanna clean them up a bit.


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Offlinejay.achS
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26736077 - 06/11/20 06:14 AM (3 years, 7 months ago)

You can also go with those ziploc 4oz mini-squares.  They worked for me through a few pressure cooker cycles.  I would definitely work inside the still air box, though.


--------------------
“One's destination is never a place, but a new way of seeing things.”  - Henry Miller

LAGM v. 2.023
LAGM2021


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: jay.ach]
    #26736157 - 06/11/20 07:32 AM (3 years, 7 months ago)

I've done everything in the SAB. I nocced 2 plates in open air for the hell of it and experimentation purposes and they both contammed. The second one I can probably get a decent transfer from since only half is contammed but I'm not gonna chance it.
One of them got all cobweb moldy and the third has a small black spot but amazing mycellium growth the rest of the plate.

This will be my first time doing a agar transfer so hoping all goes well. Definitely gonna invest in a flow good for the future.
My biggest concern so far is that I don't have much rhizomorphic growth. Mostly the fluffy white stuff. About 5 plates have rhizomorph starting towards the edges. Another reason I wanna transfer today and clean them up.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26744011 - 06/14/20 12:11 PM (3 years, 7 months ago)

So the transfers went good. Starting to see the mycellium grab hold of the new agar. I've never felt this excited lol.
Will post pictures when possible.


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InvisibleWall.E
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Registered: 06/05/20
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26744048 - 06/14/20 12:41 PM (3 years, 7 months ago)

Quote:

Hurrakrayne said:
I've done everything in the SAB. I nocced 2 plates in open air for the hell of it and experimentation purposes and they both contammed.




I always leave a plate exposed to the open air for a few minutes then seal it off to see what kind of contams are flying around my mycology room. Nothing out of the ordinary, but as long as the plates don't light up with different cultures I think I'm maintaining sterile practices. Now, if the plate ever starts going crazy with contam growth, I'll know something's fucked and I need to find it


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Life’s shit, but I’m loving it



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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: Wall.E]
    #26779500 - 06/24/20 02:08 PM (3 years, 7 months ago)

3 weeks. 6/24



So I haven't checked my plates in a few days and it's been really hot and muggy and I check today and I have these!
Those are the original plates I did transfers off and thought they were no good.  I wasn't even gonna use them. Can I still transfer these to grain even though they have pins?

Also my new plates that I transferred to have nice rhizomorph growth I just couldn't get a good enough picture.


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OfflineEntheoGod
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26779515 - 06/24/20 02:15 PM (3 years, 7 months ago)

Never thought about leaving one plate open for a few minutes and not inoculating it just to see how likely a contam would occur. Brilliant idea! Same concept as leaving a wbs jar uninoculated but just never crossed my mind 🤣.

Definitely gonna work in an SAB still but probably takes a bit of the worry away if nothing contams especially when you do things properly.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: EntheoGod]
    #26781195 - 06/25/20 05:31 AM (3 years, 7 months ago)

So those pins opened up over night and dropped spores..

Can I still use these plates to transfer to grain?
I'm starting to run my cycle of grains now.


Edited by Hurrakrayne (06/25/20 05:32 AM)


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OfflineAtmozFear
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781205 - 06/25/20 05:39 AM (3 years, 7 months ago)

That's really interesting!  Those dropped spores will probably further noc those plates... and yes, I think you can still transfer from those plates, in fact will probably do better than if there were no pins, having been consolidated for a while.


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OfflineHurrakrayne
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Re: My Second Agar Grow [Re: AtmozFear]
    #26781213 - 06/25/20 05:47 AM (3 years, 7 months ago)



One is a side view the other is a top view. There's multiple plates like that with pins and dropped spores. I'm gonna transfer directly from these plates plus some other transfer plates I have going.

Is it necessary to do g2g? Or does that just increase the amount?


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OfflineAtmozFear
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781218 - 06/25/20 05:51 AM (3 years, 7 months ago)

you always just wanna spread clean culture whenever you have time, because it saves time in the long run.


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Offlineredhandmat
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Registered: 05/09/19
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Re: My Second Agar Grow [Re: Hurrakrayne]
    #26781220 - 06/25/20 05:52 AM (3 years, 7 months ago)

Quote:

Hurrakrayne said:
3 weeks. 6/24



So I haven't checked my plates in a few days and it's been really hot and muggy and I check today and I have these!
Those are the original plates I did transfers off and thought they were no good.  I wasn't even gonna use them. Can I still transfer these to grain even though they have pins?

Also my new plates that I transferred to have nice rhizomorph growth I just couldn't get a good enough picture.




Are your plate wrapped? If nto it could explain the quick invitro pinning, as they are getting FAE. Anyways if I were you, I would clone those pins on new plates. Make sure to wrap your plates so you dont get contams in.


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