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Invisiblepsyphon
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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2749463 - 05/31/04 11:22 AM (15 years, 1 month ago)

If you're only pasteurizing and spawning you shouldn't use grass seed. I think that the grass seed could lead to contamination. If you want grass seed in there I think it'd be best to make it a part of the spawn.


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: psyphon]
    #2749713 - 05/31/04 01:55 PM (15 years, 1 month ago)

Quote:

psyphon said:
If you're only pasteurizing and spawning you shouldn't use grass seed.  I think that the grass seed could lead to contamination.  If you want grass seed in there I think it'd be best to make it a part of the spawn.



so you recommend just using dung and vermiculite? or maybe straw instead of grass seed? I could also sterilize but fear the bigger contamination risk, because I dont have a flow-hood or glowbox..
anyone any other ideas? want to get started tomorrow..
thanks  :thumbup: :smirk:


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InvisibleJopo
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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2749961 - 05/31/04 04:16 PM (15 years, 1 month ago)

Ya, would it be alright to use cow dung and vermiculate, would this work very well?


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: psyphon]
    #2750638 - 05/31/04 08:43 PM (15 years, 1 month ago)

Quote:

empath said:
Why are you so zealous about adding grass seed? Cow dung is about the best thing you can have. The only thing I can think of is for airation, but the verm can do that by just fluffing and not packing when putting in a container.

Why do you want to add grass seed so bad?




I thought it would be important, or it would not be in the recipe I found on mushmush (under methods/notes/..). Thought it might have to do something with nutrition and I have to use it to have nice flushes. So dung should suffice(?) If it is so, I will only use cowdung and vermiculite.


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Invisiblepsyphon
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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2751734 - 06/01/04 01:42 AM (15 years, 1 month ago)

If you have straw, cowdung and straw would probably be best. If it needs a bit more structure then you can add vermiculite.


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: psyphon]
    #2752118 - 06/01/04 05:05 AM (15 years, 1 month ago)

I can buy straw but dont have perfectly sterile contidions (like glow box or flowhood) and I heard of straw being contaminated more easily. Guess I will make 6 trays, 3 with grass seed/dung/verm and three with dung/verm, to test. Hope I will get any mushrooms at all  :smile:


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2752908 - 06/01/04 01:54 PM (15 years, 1 month ago)

I just finished pasteurizing the substrate for the first three trays (dung/verm only), while the grass seed I bought today is soaking water for the rest 3 trays (dung/verm/grass seed). Will sterilize tonight.
The soaking grass seed (will soak for approx. 12 hrs):


Mixed dried dung and verm in dry form, added water, let soak a little and squeezed the excess water out. Filled in two filter patched bags (substrate for 2 trays in 1 bag + substrate for 1 tray in the other)and folded the opening 2-3 times, attached clothespins:

Kept the temperature of the  water   and the  substrate between  80-84  degrees celcius and  75-83 for 2 hours and 10 minutes. That should be enough I think. The substrate was at least 80 degrees for about 1 hour, if not longer..

i let my thermometer fall and broke it, right after I finished using it  :grin: wont need one until next time anyway..

now the 2 bags are waiting in a plastic box (which will be one of the terrariums later on) to cool down, and will be spawned later today (i hope) in the cheap glow box I will try to make out of a cardboard box. (pics will come)


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Offlinenimbus
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Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2756536 - 06/02/04 01:17 PM (15 years, 1 month ago)

sterilized the substrate for the other 3 trays also, they are all together in my cupboard now, waiting.
the ratios were,

for dung/verm (per 1 liter tray): 1,5 cups of dung + 1 cup verm
for dung/verm/grass seed:        1 cup dung + 1 cup verm + 1 cup gs

dont know if that is good but we'll see..looks and feels OK to me at least. that's also the cup I used,
ordinary coffee cup. It looks like too much verm there but that's the ones floating on the water..


the glow box (without gloves) i used..better than nothing  :grin:



after spawning (sealed with an impulse sealer plus tape as there might be holes or so left:


well..for now I will just have to wait for the bags to colonize..
how often should I shake them? not often I guess..they wont start
fruiting in the bags will they??? hope not


Edited by nimbus (06/02/04 05:46 PM)


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OfflineRoger_irrelevant
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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2758835 - 06/03/04 12:03 AM (15 years, 1 month ago)

Looking good nimbus :thumbup:
Why did you sterilize the substrate though, wouldn't it be better to pastuerize?


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: Roger_irrelevant]
    #2759562 - 06/03/04 04:54 AM (15 years, 1 month ago)

I sterilized one half and pasteurized the other. But they are not the same substrate though..
I sterilized, because people said grass seed would/could lead to contamination :shrug:  and saw Una's success with sterilization and wanted to give it a try..  :wink:


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Offlinedoc34
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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2759692 - 06/03/04 06:26 AM (15 years, 1 month ago)

Looking Good nimbus,I am thinking of starting a pan cyan grow also.Keep up the log and GOOD LUCK!!!


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Offlinenimbus
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Panaeolus Cyanescens (Australian) Grow Log [Re: doc34]
    #2760044 - 06/03/04 10:53 AM (15 years, 1 month ago)

thanks everyone!
today I bought an aquarium air pump and all the stuff I need for air-axchange for later on..
got one question though,  do I have to use sterilized casing with sterilized substrate? or will pasteurizing be enough?

and

I am starting to see bright white spots of mycelium on the surface
of the substrate in the bags.  should I shake them? or leave them?

thanks for any answers, i need 'em quick!  :wink:


Edited by nimbus (06/04/04 08:05 AM)


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Offlinenimbus
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Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2768267 - 06/06/04 05:35 AM (15 years, 1 month ago)

looks like noone's interested anymore..anyway, I got some new pictures and I think and hope that I can start with casing them
tomorrow or so!  :wink:
here the bag with dung/verm/grass seed (for 2 trays):

the bag with dung/verm/grass seed (for 1 tray):

and the bag with dung/verm only (for 2 trays):

the smaller bag with dung/verm for 1 tray is not ready yet, because I shook it twice, I dont know why..  :tongue:

how can one be sure the bag is completely colonized? i mean I only see the ouside of the substrate, what about the inside?


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Invisibletrade_om
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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2768293 - 06/06/04 06:13 AM (15 years, 1 month ago)

When you see the outside is fully colonized wait 2-3 more days to be sure its all colonized. This will also le the mycelium to get much more thick and robust thus will enable it to colonize your spawn medium/case faster. This goes as well as for casings. I suggest before you case move it to a tray, let it sit with the lid on for a few days, to let the mycelium grow thick and nice then case. This will support bigger fruits.
With edibles it is done alot and the more you let the mycelium sit before premordia formation the bigger fruits you get.

EDIT - Oh yea be sure to calculate how many liters/cubic meters you got in your terrariums/fruiting chamber) and use an appropriate pump.
For say a 60liter terrarium youd want a 60literp per hour pump for 1 air exchange per hour. If you want 2 complete air exchanges per hour get a 120 liter per hour pump and so on and so forth...


Edited by trade_om (06/06/04 06:16 AM)


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2768344 - 06/06/04 07:59 AM (15 years, 1 month ago)

If I used a sterilised substrate,I would also use a sterile casing layer.But that is just my opinion.

I do have a question for you though,how long did it take for them to get from innoculation to casing?I read somewhere that Pan Cyans take a little longer to colonise and fruit than cubes do,but how much longer?

:cool: :thumbup:


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: doc34]
    #2768364 - 06/06/04 08:46 AM (15 years, 1 month ago)

I am using a partly sterilized casing (peat/verm/calcium carbonate) this time (cooked in PC for 1 hour). Think it will be good enough, mushmush suggests..

I did not case them yet, so I cant tell you how long it took till casing but it took 2 weeks for full colonization of jars (720ml)
and 4-5 days for the colonization of the substrate bags mixed with colonized birdseed (the bags are not 100% colonized yet but 70-80% I guess). In 2-3 days it should be the time for pouring it into trays and letting them wait a day or so, the case them and so on..

BUT- I read that pan cyans colonize fast and fruit fast too, they just seem weaker to the eye


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Edited by nimbus (06/06/04 09:01 AM)


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: nimbus]
    #2774075 - 06/08/04 09:46 AM (15 years, 1 month ago)

I just moved the dung/verm/grass seed substrate to the trays and covered with alu-foil. Both bags looked like this:


I had planned to use 3 but there was enough to fill 4!! so I did 4..
(2 one liter, 1 1.25 liter and 1 i guess is almost 1 liter) Didnt case them yet..will wait till tomorrow or so, just to let the mycelium get stronger again. Dont have the patience to let them wait 2-3 days before casing.
The dung/verm substrate seems to be colonizing slower. Should be ready in a few days..


Edited by nimbus (06/08/04 09:54 AM)


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Re: Panaeolus Cyanescens (Australian) Grow Log [Re: PoC]
    #2777807 - 06/09/04 01:48 PM (15 years, 1 month ago)

Just PC some grain and use that..... It wont be 100% sterile but itll be colonized befor you know it.. Or you could jsut get soem grass seed


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Casing time!! [Re: Legoulash]
    #2778909 - 06/09/04 08:24 PM (15 years, 1 month ago)

thanks for the advice..
I cased the 4 trays with a 1 cm thick layer (or 1.3 cm-something like that) a few minutes ago!
They have been waiting for 34 hours in the trays, closed with aluminum foil, allowing the mycelium get a little stronger before being cased. This is how they looked, sorry for the poor quality, I cant make very sharp pics with my camera, dont know why.. (3.1 megapixel HP Photosmart 435)

and after casing:

they were all in the same condition so I took photos of only one..
Gave the casing a light misting because it seemed to have dried out slightly, waiting in the jars for 3 days.

...

took a look at the other 2 bags filled with dung/verm (spawned with birdseed) and decided to transfre them to trays too..again, I saw that I did some miscalculation, instead of 3 trays I have 3 trays + a 1,5 liter PET water bottle layed on its side, cut to make a tray out of it, because had no more trays  :grin: photos of this will come on the day of their casing, which should be friday sometime..


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Edited by nimbus (06/10/04 11:16 AM)


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Re: Casing time!! [Re: nimbus]
    #2784756 - 06/11/04 02:09 PM (15 years, 1 month ago)

I hope for you they come  :goodluck: :goodluck:


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Panaeolus

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