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Offlineredhandmat
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What do you think about this germination agar plate?
    #26734253 - 06/10/20 12:16 PM (3 years, 7 months ago)



GT print to Malt extract + agar + antibiotics (prior bacterial issues with another print, that's why) 10 days ago. As you can see I have already taking two transfers. But wanted to check with you guys since this looks a bit too much like cobweb, however the growth hasn't been overnight, but actually gradual over 10 days. And I have a second plate that looks much like this one.

My third plate, without antibiotics looks like this:



So, what do you guys think? do we have myc here?


Edited by redhandmat (06/13/20 08:39 AM)


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InvisibleRoger Clemency
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Re: What do you think about this germination agar plate? [Re: redhandmat]
    #26734282 - 06/10/20 12:27 PM (3 years, 7 months ago)

It’s hard to tell what’s going on there. Did you scrape a print over top of those plates or something?

If you just swipe a single line or a zigzag it’s a lot easier to see what you’re working with and you know that anything along those lines is something you swiped to the plate. Anything outside is a satellite colony of mold or whatever that got there some other way.

Those first pictures look suspect to me. Might be because of the antibiotic stuff idk I’ve got no experience with it. The last picture looks kinda like a whole bunch of chaotic cube myc germination points but anything could be in there with all that mess :smile:

Less is more at this stage. I’d transfer out of that last plate and just see what happens. And maybe start some new plates with just agar/lme


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Offlineredhandmat
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Re: What do you think about this germination agar plate? [Re: Roger Clemency]
    #26734336 - 06/10/20 12:46 PM (3 years, 7 months ago)

Quote:

Roger Clemency said:
It’s hard to tell what’s going on there. Did you scrape a print over top of those plates or something?

If you just swipe a single line or a zigzag it’s a lot easier to see what you’re working with and you know that anything along those lines is something you swiped to the plate. Anything outside is a satellite colony of mold or whatever that got there some other way.

Those first pictures look suspect to me. Might be because of the antibiotic stuff idk I’ve got no experience with it. The last picture looks kinda like a whole bunch of chaotic cube myc germination points but anything could be in there with all that mess :smile:

Less is more at this stage. I’d transfer out of that last plate and just see what happens. And maybe start some new plates with just agar/lme




Yes scraped print with inoculation loop. The last plate I did do line patterns with, and the initial points of growth were all within that pattern of lines. But I wasnt expecting to get growth on all lines , and so fast. My prior plates from another print took much longer to germinate. I was used to plates taking at least a week to show initial grow, nothing this fast. Anyways, I'll transfer from the last plate and see what happens.

To be honest I have no experience with antibiotics either. Its just that my prior print gave me a lot of trouble. So I wanted to try my hand at mixing in a bit of antibiotics on the plates to see what happens.


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Offlineredhandmat
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Re: What do you think about this germination agar plate? [Re: redhandmat]
    #26734386 - 06/10/20 01:01 PM (3 years, 7 months ago)

I should add that Im also a little suspicious of the antibiotics plates. Primarily due to the way you have to work with them. Basically you cant PC the antibiotics. So I sterilized water in a glass container, dropped a pill of antibiotics after the water cooled down. Then used a syringe and squirted out a few drops on top of the plate after inoculation.

My thinking was that the antibiotics would make it hard for bacteria to grow, and then I could transfer to a regular MEA plate (which I now did). However, I didn't think that molds are not hindered by antibiotics at all. So its possible that the antibiotic plates have germinated molds :S Thats why Im hoping for some good feedback from the community.


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OfflineA.k.aM
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Re: What do you think about this germination agar plate? [Re: redhandmat]
    #26734696 - 06/10/20 03:23 PM (3 years, 7 months ago)

Those all look fine to me.


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LAGM2020


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Offlineredhandmat
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Re: What do you think about this germination agar plate? [Re: A.k.a]
    #26735952 - 06/11/20 03:16 AM (3 years, 7 months ago)

Quote:

A.k.a said:
Those all look fine to me.




Thank you Aka! Ill keep at it then :smile:


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Offlineredhandmat
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Re: What do you think about this germination agar plate? [Re: redhandmat]
    #26741274 - 06/13/20 08:02 AM (3 years, 7 months ago)

So the transfers I took above now look like this after 2-3 days:



So Im thinking of taking the second transfer soon from the second picture, since its showing more rhizo growth than the first picture. What are your opinions?

Im thinking 10-12 o'clock and 4-7 o'clock. or should I wait longer before taking transfers?


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InvisibleRoger Clemency
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Re: What do you think about this germination agar plate? [Re: redhandmat]
    #26741566 - 06/13/20 10:39 AM (3 years, 7 months ago)

The Band of the Red Hand...lookin good mang.

And yeah if i wasn't being lazy i'd take a few transfers off that thing right now.


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Sour grapes, sweet revenge
Heaven starts right where hell ends


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