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Offlinepsycho_nauticus
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Questions with developing in efficiency...?
    #26729128 - 06/08/20 02:49 PM (3 years, 7 months ago)

I have always been concerned about these issues that would make me more efficient, and save time and energy:

1) How long is sterilized grain good for? In my case, my choice of grain is rye berries, but I always prepare my grain whenever I am ready to inoculate. Could I instead just make a bulk prep of jars of rye berries early and keep them for whenever I am ready for inoculation? If so, how long would jars of sterilized rye berries after a PC cycle be good for? Or is it best practice to just make jars of grain when I am ready to use them?

2) When transferring and doing agar work, is it okay to let your culture touch the edges of the glass/plates? I have always been under the assumption that when they touch the edges it invites and starts contamination, so I have been sticking them in the refrigerator to stall growth when it gets near so I don't risk any contamination of any cultures if I am not yet ready. Am I being too worried and overly cautious about this? Because I am thinking I have to use them to inoculate before they hit the edge or should I just keep putting them in the refrigerator until I am ready to use them for inoculation?

I am looking to develop in efficiency. Thank you for your input and answers.


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Invisible7even
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Re: Questions with developing in efficiency...? [Re: psycho_nauticus]
    #26729139 - 06/08/20 02:56 PM (3 years, 7 months ago)

2. you can let it touch the outer edges. No big deal.


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Offlinepsycho_nauticus
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Re: Questions with developing in efficiency...? [Re: psycho_nauticus]
    #26729778 - 06/08/20 07:22 PM (3 years, 7 months ago)

3) After taking out the refrigerator to slow growth, how long does it usually take before the culture starts to growing again? How many days before should you take it back out to room temperature to start growth again before inoculating?


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