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OfflineLogicalElk
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Registered: 05/27/20
Posts: 5
Last seen: 3 years, 4 months
Working on agar plates advice [With Pics!]
    #26726653 - 06/07/20 01:43 PM (3 years, 7 months ago)

Hi guys!

Well I am making my first baby steps working on agar.

A) One of my projects was salvaging a 1,5 year old Colombian variety spore syringe:

I directly nocced grain (as I used to do), after two weeks or so got a nasty bacterial jar but with some mycelium, so I took the best looking grain from top with sterile tweezers and transfered to gentamicin BRF agar. Then made another transfer which is the following:



My question on this experiment are:
A1) Does it look Clean?
A2) Why is the mycelium so faint?
A3) If I keep doing trasnfer will it get thicker?
A4) Can I start doing a2g transfer and start working on grains or should I wait till it gets more vigorous?

B) Luckily a friend o mine gave me another spore print (Mex.Pal.) so now that I am learning the ropes of agar work I decided to test the spores on a plate:



My question on this experiment are:

B1) Again: Does it look Clean?
B2) Do you guys think is a good idea to get a wedge where te red arrow is?
B3) Again: Can I start doing a2g transfer and start working on grains or should I wait till it gets more vigorous?


Thank you guys very much for your knowledge! My best wishes to you all :grin:

Namaste


Edited by LogicalElk (06/07/20 01:49 PM)


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OfflineLogicalElk
Stranger

Registered: 05/27/20
Posts: 5
Last seen: 3 years, 4 months
Re: Working on agar plates advice [With Pics!] [Re: LogicalElk]
    #26727206 - 06/07/20 06:03 PM (3 years, 7 months ago)

Edit: re uploaded the pictures.


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OfflineLogicalElk
Stranger

Registered: 05/27/20
Posts: 5
Last seen: 3 years, 4 months
Re: Working on agar plates advice [With Pics!] [Re: LogicalElk]
    #26728506 - 06/08/20 09:33 AM (3 years, 7 months ago)

My aplogies, just realised that I bumped before the 24 hrs mark. ;/


Edited by LogicalElk (06/08/20 09:36 AM)


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