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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Isolating for true monoculture
#26724180 - 06/06/20 01:40 PM (3 years, 8 months ago) |
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So I’m in a point in my agar work where I’m interested in creating monocultures, I’ve got prep and transfer reined in. So when selecting I look for the fastest growing rhizomorphic growth if it’s extremely white that’s a plus. In this is there any other criteria I should be looking for in the sectoring or hypea? To find true monos is it necessary to have a microscope?
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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When ran from spores there are 100-1000s of strains present, as we isolate and transfer we are reducing those numbers. As you keep transferring from a2a or clones to agar you keep reducing the numbers. It just takes time and patience.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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So even within a clone there are going be more strains? I thought once you cloned a fruit it held single characteristics. What is the best method for selecting for yield?
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Strainsfordaze


Registered: 05/10/18
Posts: 669
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Even when running clones if it wasn’t near being a monoculture it will still hold many strains. Running MS agar to grains, transfer until clean, put to grains fruit them, clone the winners, isolate growth, fruit, etc... time, patience and willingness. Every time you transfer, even with clones you are reducing the number of strains.
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Dirtytaco666
Stranger
Registered: 06/06/20
Posts: 1
Last seen: 3 years, 8 months
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Hello my name is seth, Obviously new to this group and would like if you guys pointed me in the right direction if this isnt the right place to post this. Kind of a hard forum to navigate to start a thread. My story as it's not important to my question it may be of some interest to readers. I have always been weary of psychedelics as I was raised in a very closed minded family with strict rules for the men in my household. Since high school I have experimented with drugs finding weed to be be my go to. Cocaine later on found itself to me number one then to Meth as it's cheaper and lasts longer. Well the first time I tried shrooms I didnt know how much to take so i ate an eighth. Well right before the peak of my high I was having so much fun I decided that the three giant mushrooms I had left (5 inches with 1 to 2 inch caps) looked tasty (I like the taste). Well the next hour went by very fast and soon didnt know who I was and just couldn't believe that I was a human being. Mostly because the entire idea just didnt sound likely. Well apparently that's called an ego death if I'm not mistaken. Well that was also the last time I ever did Meth. Shrooms changed my life. I'm far more humble less narcissistic and more loving. I have been sober three years and own my own successful business repairing power sports like ATV's, UTV's, Motorcycles, and PWC's. Now to my conclusion; so while taking another big dose of mushrooms a little over two months ago I apparently decided that I'd buy a grow kit from Midwest Grow kits. I bought 4 spore syringes from premium spores. I didnt remember this when all of a sudden I get these mysterious boxes a little over a week later. Well I thought shit I'd give it a shot. At least it ll be fun. Well I was wrong. It's the greatest thing ever stumbled upon. So all half the jars colonized and I had two that were my strongest girls contaminate in their jars with two separate contaminants. One green Trich and one black.... well since then all colonized jars are in the mono tub and are on their second flushes. The rest are almost colonized waiting for these cakes to be spent and they will have their turn to spread their legacy. With no other. Contaminants at all. I'd say that's pretty good for my first time but I'll keep doing it and try to get better and better. Anyways the two contaminated jars I have kept in my shop miles away and always wash my hands after handling and haven't even opened them up yet. Just wanted to do an experiment and see how fast the contams spread. Well something I never expected happened. Both jars have not spread more than when I first noticed the contaminates which goes against everything I have learned online. In fact the contaminates have started being overtaken by the Mycelium and two weeks ago I noticed pins. Now I have tons of mushrooms growing pressed against the glass. On being nearly the size of the jar itself. That leads me to my question and subsequently statement. I haven't given either jar any water whatsoever. They have just produced their own moisture and they are thriving as well as getting rid of if not attacking the mold to the point of remission! What the hell? Why would the Mycelium fight the mold I thought that never happens? How can I be getting large ass fruits when they get no moisture no air and being in my cupboard no light unless I take them out to examine the state of growth!? And can I take spore prints from the jar with trich in it? Obviously I wont touch the one with black mold. Thank you guys and thank you for all you guys do for people like me by spreading the knowledge. You guys are the real heroes.
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Pastywhyte
Say hello to my little friend


Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Isolating for true monoculture (moved) [Re: PiptheGreAtest]
#26727352 - 06/07/20 07:04 PM (3 years, 8 months ago) |
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This thread was moved from Advanced Mycology.
Reason: Wrong board, not an advanced topic. Do not mention vendors in reference to cultivating on these boards please.
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