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OfflineSockadin
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Can someone tell me if this LC is clean?
    #26723531 - 06/06/20 07:49 AM (3 years, 7 months ago)

Just curious if this LC looks ok to you all..



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InvisibleSubnet Mask
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26723534 - 06/06/20 07:50 AM (3 years, 7 months ago)

:thumbup:


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Invisiblemicelio
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Re: Can someone tell me if this LC is clean? [Re: Subnet Mask]
    #26723547 - 06/06/20 08:01 AM (3 years, 7 months ago)

All the floating small pieces doesn't look right to me. But with just one picture It not easy to tell.

I wish I could give you a better opinion.

Also It looks to be on the dark side.


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OfflineSockadin
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Re: Can someone tell me if this LC is clean? [Re: micelio]
    #26723577 - 06/06/20 08:19 AM (3 years, 7 months ago)

It was a joke. It's mold.


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Invisiblemicelio
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26723646 - 06/06/20 09:00 AM (3 years, 7 months ago)

Haha....!

I was saying to myself, wow.. Sockadin has been around for long time and knows the business well. Why would he be asking a question like that..?

Hey you really had me scratching my head.. 

I love a little humor

Thanks..


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OfflineSockadin
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Re: Can someone tell me if this LC is clean? [Re: micelio]
    #26723656 - 06/06/20 09:03 AM (3 years, 7 months ago)

It is was actually a shocker cause it was properly sterilized grain soak water knocked up with a clean agar wedge and thrown in the back of the shelf for a couple of weeks.

Either my flowhood sucks, the wedge was hiding a contam, ir those plastic lids are crap.


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InvisibleLadysKnight
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26723670 - 06/06/20 09:11 AM (3 years, 7 months ago)

Test it on agar to be sure.


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Invisiblemicelio
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26723679 - 06/06/20 09:21 AM (3 years, 7 months ago)

Door number 2

The wedge was hiding a contam...


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OfflineSockadin
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Re: Can someone tell me if this LC is clean? [Re: micelio]
    #26723696 - 06/06/20 09:31 AM (3 years, 7 months ago)

That scares me.


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OfflineA.k.aM
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26723897 - 06/06/20 11:20 AM (3 years, 7 months ago)

Plastic lid!

Actually I’ve never used those, thought about it cuz one piece would be nice. 

But the cheap media bottle lids got me paranoid. I spin some serious cling wrap around them now.


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Invisiblemicelio
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Re: Can someone tell me if this LC is clean? [Re: A.k.a]
    #26724022 - 06/06/20 12:12 PM (3 years, 7 months ago)

I like having a plastic bag tent covering my plastic Lid.

The plastic tent has vent holes covered with micropore tape.



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OfflineA.k.aM
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Re: Can someone tell me if this LC is clean? [Re: micelio]
    #26724034 - 06/06/20 12:16 PM (3 years, 7 months ago)

Don’t plastic lids get enough ge as they come?? I thought that was why people used them.

I’ve been thinking about doing syringe filters on some of my lids too, they seem convenient.


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LAGM2020


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OfflineSockadin
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Re: Can someone tell me if this LC is clean? [Re: A.k.a]
    #26724045 - 06/06/20 12:20 PM (3 years, 7 months ago)

Syringe Filters on metal lids work for me.


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Invisiblenix21
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Re: Can someone tell me if this LC is clean? [Re: A.k.a]
    #26724049 - 06/06/20 12:21 PM (3 years, 7 months ago)

I think you way overcolonized this one.. i can see some knots on surface


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OfflineSockadin
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Re: Can someone tell me if this LC is clean? [Re: nix21]
    #26724067 - 06/06/20 12:34 PM (3 years, 7 months ago)

It's mold on the surface. It was a joke . Sorry.


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Invisiblegizmo1
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26724071 - 06/06/20 12:36 PM (3 years, 7 months ago)

Looks good to me. I'd use that as a master and make some more liquid cultures.

Quote:

Sockadin said:
Syringe Filters on metal lids work for me.



This is what I use too. This or unmodified media bottle.


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OfflineSockadin
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Re: Can someone tell me if this LC is clean? [Re: gizmo1]
    #26724082 - 06/06/20 12:40 PM (3 years, 7 months ago)

Quote:

gizmo1 said:
Looks good to me. I'd use that as a master and make some more liquid cultures.



:aweohyou:


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Offlinebrikogen
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26724129 - 06/06/20 01:12 PM (3 years, 7 months ago)

Don't be discouraged by a single failure, we all started from the bottom you will get there some day.


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Invisiblemicelio
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26724137 - 06/06/20 01:18 PM (3 years, 7 months ago)

Quote:

Sockadin said:
Quote:

gizmo1 said:
Looks good to me. I'd use that as a master and make some more liquid cultures.



:aweohyou:








:aweohyou::aweohyou:




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Invisiblegizmo1
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Re: Can someone tell me if this LC is clean? [Re: micelio]
    #26724162 - 06/06/20 01:30 PM (3 years, 7 months ago)

Quote:

micelio said:
Quote:

Sockadin said:
Quote:

gizmo1 said:
Looks good to me. I'd use that as a master and make some more liquid cultures.



:aweohyou:








:aweohyou::aweohyou:






:aweohyou::aweohyou::aweohyou:


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Invisiblenix21
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26724205 - 06/06/20 01:53 PM (3 years, 7 months ago)

Quote:

Sockadin said:
It's mold on the surface. It was a joke . Sorry.




I followed the joke xD rly not that dumb


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OfflineA.k.aM
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Re: Can someone tell me if this LC is clean? [Re: nix21]
    #26724517 - 06/06/20 04:30 PM (3 years, 7 months ago)

:lol:

This thread has far surpassed expectation


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LAGM2020


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InvisibleSmartattack
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Re: Can someone tell me if this LC is clean? [Re: A.k.a]
    #26724937 - 06/06/20 08:01 PM (3 years, 7 months ago)

I could use a culture sample for amending the spawn in my next Planet of apes grow. I think my new culture is going to be too clean and we cant be having that.


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OfflineA.k.aM
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Re: Can someone tell me if this LC is clean? [Re: Smartattack]
    #26724951 - 06/06/20 08:06 PM (3 years, 7 months ago)

You can name the culture “death star divot”


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LAGM2020


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OfflineSockadin
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Re: Can someone tell me if this LC is clean? [Re: A.k.a]
    #26725744 - 06/07/20 06:29 AM (3 years, 7 months ago)

Mold the #1 pinning trigger.

We might need a new thread titled that.


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OfflineA.k.aM
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26725753 - 06/07/20 06:35 AM (3 years, 7 months ago)

Man I just woke up to massive trich on a big PE tub that hadn’t even started knotting yet.

Never had a tub not get to the first flush.

I’m afeared for the rest of them now. :uhoh:


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InvisibleMateja
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Re: Can someone tell me if this LC is clean? [Re: A.k.a]
    #26725768 - 06/07/20 06:47 AM (3 years, 7 months ago)

I'm currently doing an experiment to determine if I can make 100% clean LC syringes without needing to test them first. I'll let the LC colonize fully until the mycelial mat on the surface starts to pin (meaning it's Clean) and then I'll just poke a needle through the surface and aspire the LC that's underneath the 'protective layer'. Maybe even use this to inoculate next generation LC and repeat the process to determine that the LC is still clean. I have 6 LCs almost fully colonized right now. 5 of them look clean so far but in one of them mycelial growth has halted and the rye water has gone turbid.


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OfflineSockadin
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Re: Can someone tell me if this LC is clean? [Re: A.k.a]
    #26725784 - 06/07/20 07:03 AM (3 years, 7 months ago)

Welcome to Cultivation! Broke your contam cherry!

Happens to everyone.

Mateah: keep us posted on how it turns out. I hate LC's I always germinate mold.


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InvisibleMateja
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Re: Can someone tell me if this LC is clean? [Re: Sockadin]
    #26725795 - 06/07/20 07:14 AM (3 years, 7 months ago)

Fo who, I'll probably swirl the LC's for the last time tomorrow and then let them rest until they pin. I'll make a thread and post the results. And btw the cakes I'm fruiting now that we're nocced with LC are the foret time I'm using LC for a grow and so far Im really enjoying LC's. Before that I've only cultivated them for the sake of the broth for experiments and observations.


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Invisiblegizmo1
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Re: Can someone tell me if this LC is clean? [Re: Mateja]
    #26727834 - 06/08/20 12:04 AM (3 years, 7 months ago)

So has anyone smelled a clean LC yet?


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