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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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DUMBASS: SHIP Idiocy
#26722152 - 06/05/20 03:03 PM (3 years, 8 months ago) |
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FOAF is new to all this, and painstakingly went out of his way to research as much as possible, built a reasonably solid SAB, bought jars and lids with SHIPs, and obtained spore samples from a very well thought of Shroomery vendor. SAB was sprayed with Lysol and left to sit for ten minutes; PC sterilized grain spawn was placed in SAB after spraying, but during 10 minute period.
With gloved hands and Tyvek sleeves, and after rubbing down with 190 proof alcohol, FOAF gently brought syringe into SAB, flamed his needle, and injected spore solution into sterilized grain spawn. FOAF was then devastated he forgot to wipe SHIP on lids with alcohol. Further devastated to see here on Shroomery that LC should have been used, rather than spore solution directly. FOAF is learning from his mistakes.
What would you estimate FOAFs chances are of clean colonization of his 1 pint grain spawn jars given above?
Thanks...
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 hour, 25 minutes
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Re: DUMBASS: SHIP Idiocy [Re: Abilor] 1
#26722205 - 06/05/20 03:34 PM (3 years, 8 months ago) |
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Depends on how clean the syringe is. I’ve used syringes from a big vendor a ton of times straight to grain and never had a problem, but other people lose every jar.
Also lc isn’t necessary, it’s pretty advanced and super easy to contaminate, and requires agar.
Syringe to grain is fine imo, but if you want to go the best route you need agar. Or brf paste.
If the jar doesn’t work out look into pf tek.
Swim, foaf, my cats neighbors mom
--------------------
LAGM2020     
Edited by A.k.a (06/05/20 03:37 PM)
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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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Re: DUMBASS: SHIP Idiocy [Re: Abilor]
#26722206 - 06/05/20 03:34 PM (3 years, 8 months ago) |
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Did some reading just now, and FOAF thinks he knows more about how this will go, but still interested in hearing what the wizards have to say...
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: DUMBASS: SHIP Idiocy [Re: Abilor] 1
#26722207 - 06/05/20 03:36 PM (3 years, 8 months ago) |
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Did you remove foil from the lids just prior to inoculation?..err ur FOAF
MS is a genetic crapshoot and even the best vendors have sold contaminated syringes inadvertently.
Agar FTW
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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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Re: DUMBASS: SHIP Idiocy [Re: A.k.a]
#26722215 - 06/05/20 03:40 PM (3 years, 8 months ago) |
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Thx! So I just read that a reputable syringe needle used the first time is so smooth, it is pretty resistant to picking up grubby things from the port. After a few uses, the tip goes raggedy and dull, so microbeasties start riding in. Luckily, FOAF made multiple jars just in case he loses a few, so hurray risk management.
In the future, because FOAF has an SAB, he will instead wipe down/Lysol the SAB, add the jars, wait ten minutes, then pop tops and inoculate direct from syringe without needle, so tip touches NOTHING. SHIP is more for if you just can't access a SAB apparently.
FOAF thought brewing was fussy; sanitizing a bucket and tools, and pitching yeast into wort seems like child's play now lol...
Edited by Abilor (06/05/20 03:42 PM)
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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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Quote:
ModularMind said: Did you remove foil from the lids just prior to inoculation?..err ur FOAF
MS is a genetic crapshoot and even the best vendors have sold contaminated syringes inadvertently.
Agar FTW
FOAF was an overeager jackass, and peeled the foil last night, fully expecting to wipe down prior to injection.
FOAF laments his life choices, but only makes mistakes once... Thanks for helping him.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: DUMBASS: SHIP Idiocy [Re: Abilor] 1
#26722217 - 06/05/20 03:42 PM (3 years, 8 months ago) |
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Where do people even find shit telling them to uses FOAF anymore.
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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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Re: DUMBASS: SHIP Idiocy [Re: bodhisatta]
#26722219 - 06/05/20 03:43 PM (3 years, 8 months ago) |
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Quote:
bodhisatta said: Where do people even find shit telling them to uses FOAF anymore.
Is it now passé? What's wrong with a little security lol?
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ModularMind
M.P.F.



Registered: 02/09/10
Posts: 7,902
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Re: DUMBASS: SHIP Idiocy [Re: bodhisatta] 1
#26722220 - 06/05/20 03:45 PM (3 years, 8 months ago) |
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The shroomery, bod! They learned it from reading the shroomery!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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It's 0 security at all.
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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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Re: DUMBASS: SHIP Idiocy [Re: bodhisatta]
#26722237 - 06/05/20 03:56 PM (3 years, 8 months ago) |
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Quote:
bodhisatta said: It's 0 security at all.
How do we know this?
And are you recommending TOR/VPN instead, or is plain ol' HTTP at one's ISP a non-issue given these troubled times?
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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That's not security i dont think : ) The risk is them finding you in real life based on info you put here. Not very likely unless you're doing something really stupid. And if they find you in real life it doesnt matter what acronym or whatever you used in posts because they will have real live evidence.
Read up on agar though if you plan on growing more than once or twice. It's basically the basis of mushroom cult of all kinds.
A SAB works on the still air principle. Lysol wont hurt i guess but it is flammable and smells terrible to me, i hate breathing that stuff in. Just spraying down the sides lightly with water is all thats needed. you just want things that land there to get stuck, you're not really trying to kill them all.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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Jesus Christ, why am I not using agar... I've mixed up DME for cultivating yeast for brewing hundreds of times... It never occurred to me I could sterilize it in the PC, and pour the plates in my SAB after wiping it down with Star-San or the like.
Thanks everybody!
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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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Re: DUMBASS: SHIP Idiocy [Re: Abilor]
#26722253 - 06/05/20 04:05 PM (3 years, 8 months ago) |
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Got it @Roger Clemency. Since this is all for personal fun, and very private, it should fly under the radar. Hopefully all legal soon anyway.
Amazing how some people don't keep their goddamn mouth shut and are only looking for a quick buck.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: DUMBASS: SHIP Idiocy [Re: Abilor] 1
#26722273 - 06/05/20 04:16 PM (3 years, 8 months ago) |
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Star-san is garbage. Search my posts about it probably could use ddbsa as a keyword too.
And if you get caught its from IRL things
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Abilor
Myconaut

Registered: 05/15/20
Posts: 95
Last seen: 2 years, 7 months
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Re: DUMBASS: SHIP Idiocy [Re: bodhisatta]
#26722283 - 06/05/20 04:24 PM (3 years, 8 months ago) |
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Quote:
bodhisatta said: Star-san is garbage. Search my posts about it probably could use ddbsa as a keyword too.
And if you get caught its from IRL things
No doubt; for brewing it was ok, but I would never use it for this sort of thing.
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